LOCUS       sequence-380328-        8209 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive retroviral expression of PRDM14.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8209)
  AUTHORS   Kim M, Ly SH, Xie Y, Duronio GN, Ford-Roshon D, Hwang JH, Sulahian 
            R, Rennhack JP, So J, Gjoerup O, Talamas JA, Grandclaudon M, Long 
            HW, Doench JG, Sethi NS, Giannakis M, Hahn WC
  TITLE     YAP1 and PRDM14 converge to promote cell survival and tumorigenesis.
  JOURNAL   Dev Cell. 2022 Jan 24;57(2):212-227.e8. doi: 
            10.1016/j.devcel.2021.12.006. Epub 2022 Jan 5.
  PUBMED    34990589
REFERENCE   2  (bases 1 to 8209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2021.12.006"; journalName: "Dev Cell"; date: 
            "2022-01-24- 24"; volume: "57"; issue: "2"; pages: "212-227.e8"
COMMENT     Sequence Label: pMSCV puro PRDM14
FEATURES             Location/Qualifiers
     source          1..8209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     339..362
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(411..437)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(2151..2174)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      2174..2183
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(2202..2618)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(2218..2234)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(2250..2272)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    2685..3026
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     LTR             3090..3606
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(3806..3825)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3953..3975)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4169..4188)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4288..4310
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4348..4366)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4434..4538
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4539..4607,4608..5399)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4757..4776)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5570..6158
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6059..6078
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6312..6329
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6446..6467
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6482..6487,6488..6505,6506..6512)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6520..6536
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     LTR             6698..7212
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     CDS             complement(7312..7911)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7395..7415)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7892..7911
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     complement(7980..7999)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 cccctgagcc cgcccctgag cccgcccccg gacccacccc ttcccagcct ctgagcccag
       61 aaagcgaagg agcaaagctg ctattggccg ctgccccaaa ggcctacccg cttccattgc
      121 tcagcggtgc tgtccatctg cacgagacta gtgagacgtg ctacttccat ttgtcacgtc
      181 ctgcacgacg cgagctgcgg ggcggggggg aacttcctga ctaggggagg agtagaaggt
      241 ggcgcgaagg ggccaccaaa gaacggagcc ggttggcgcc taccggtgga tgtggaatgt
      301 gtgcgaggcc agaggccact tgtgtagcgc caagtgccca gcggggctgc taaagcgcat
      361 gctccagact gccttgggaa aagcgcctcc cctacccggt agaattccta agcgtaatct
      421 ggaacatcgt atgggtagta gtcttcatga aacttcatgt gggagtagaa tgtttcttga
      481 tctgagaaga ttttcccaca gatgctgcat gagcagccat cgtcctcctt gtgtgaacgc
      541 cggacatggc tgtcatgggc agcatgggat gcaaaagatt caccacagta cttgcatttg
      601 aagggcttct ccccggagtg ctgcctgatg tgtgtgcgga gtatgctgga ggctgtgaac
      661 ctcttagtac aatacacaca ctggtatggt ctgtctccag agtggactcg catgtgtttg
      721 tttaggctgg aagactgaga gaaacatttc ccacatgtag aacacttgtg aggccggtgc
      781 ttctcatgaa catgaagaat gtggatccga agccggtccc gcttcccaaa ggatcgtttg
      841 cagagagaac agggaaattt cctatcgccc ttgtccacac agggggtgta cttgaggtgc
      901 ttatctctgt aatatttgta ggtaaatacc ttcccacatc tttcacatct gtagccttct
      961 gcagactctt cagagggccc agatggctgc ttccccggct ctgtgacctg aaggctcaca
     1021 ggaatatcca gaaatttctc atagcagtct ccataccaca caaggagctc ttggttctga
     1081 tgaatctctt tgcagctctc ataaaatata tgcccttgac actgcacagc aactaggttc
     1141 tgctccttgg ggaagcgggc acagttgaca taggacatcc agttccccgt acctcctttt
     1201 ccacctataa agtggctcaa atgaccatct tcaaagatct cccacatcac agaattgtct
     1261 ccgtaggtct tcacttcact ggcattgacc actttacctt gaaagggccc aaacctgact
     1321 cctttggcga taaaactact gcagaacaca ccaaaatgtg ggacttcacc aaacaccgtc
     1381 tgcatgaggc atagaccttc tggaagttga agggagtctt catccagagt ttgaggaaga
     1441 gaatcagatc cagagctgtc tggggggacc aggaggcctg aaatcgcatg gtgcaggctg
     1501 gctgggtgct ccaggctggg agtgaccccg tacagaacga agtgcaggtc ctcctccgtg
     1561 aactggaacc gagcagggga cttcccttct tggttggagg gtttgggacc atcctctgac
     1621 tgcttgctgg gtgagcaagg taataactgg gaggtcctca gcccctcagg taacagagaa
     1681 gcatccgcag ggggcggtgg aattaaagtg tcaggtccac aacacgggcc actctcgttg
     1741 tcgccaccaa tgatttggtg gcccagatct tcactgctgg cacccgcgta ctcgtggctg
     1801 ctgctcagga agggcggcac ttccctgggg acgtggggaa ttgggtacca cggtggcagc
     1861 ttgctgtacc agggcagatc gtagagaggc tccctctgta ggcccagacc cgggctcagc
     1921 aagggaggcg ccatccggaa ggggaagggg ggcatggcgg gggcagcaga cgctgcggcc
     1981 tccagctgcc ggaaaggttg gaagtcttcc tccacggtgg ccaggctgtt tctgtagtgt
     2041 ccataggacg ggaaaggcgt gtagtacgcg gccaggttct gcgggctgct ctccggcggg
     2101 tagcacacct tgtcctgagg cacggcctca cttggccggg gtagagccat cttgtcgtca
     2161 tcgtctttgt agtccatggt ggcctcgaga gatctaattc cggcgcctag agaaggagtg
     2221 agggctggat aaagggagga tcgaggcggg gtcgaacgag gaggttcaag ggggagagac
     2281 ggggcggatg gaggaagagg aggcggaggc ttagggtgta caaagggctt gacccaggga
     2341 ggggggtcaa aagccaaggc ttcccaggtc acgatgtagg ggacctggtc tgggtgtcca
     2401 tgcgggccag gtgaaaagac cttgatctta acctgggtga tgaggtctcg gttaaaggtg
     2461 ccgtctcgcg gccatccgac gttaaaggtt ggccattctg cagagcagaa ggtaacccaa
     2521 cgtctcttct tgacatctac cgactggttg tgagcgatcc gctcgacatc tttccagtga
     2581 cctaaggtca aacttaaggg agtggtaaca gtctggccct aattttcaga caaatacaga
     2641 aacacagtca gacagagaca acacagaacg atgctgcagc gctgcagcag acaagacgcg
     2701 cggcttcggt tccaaaccga aagcaaaaat tcagacggag gcgggaactg ttttaggttc
     2761 tcgtctccta ccagaaccac atatcctgac ggggtcggat tccacatcga ctcccttcct
     2821 caggtcgggc cacaaaaacg gcccccaaag tccctgggac gtctcccagg gttgcggccg
     2881 ggtgttcaga actcgtcagt tccaccacgg gtccgccaga tacagagcta gttagctaac
     2941 tagtacagac gcaggcgcaa acattagatg ccggcacaaa cacgcacaaa gacagagaca
     3001 gacacgaaac gaccgctggc cagcttacct cccggcgggg gggtcggtgg tccctgggca
     3061 ggggtctcca aatctcggtg gaacctccaa atgaaagacc cccgaggtgg gcagtcaatc
     3121 aatctgagga gaccctccca aggatcagcg agtccacgat tcggatgcaa acagcaagag
     3181 gctttattgg gaatacgggt acccgggcga cgcagtctat cggaggactg gcgcgccgag
     3241 tgaggggttg tgggctcttt tattgagctc ggggagcaga agcgcgcgaa cagaagcgag
     3301 aagcgaactg attggttagt tcaaataagg cacagggtca tttcaggtcc ttggggcacc
     3361 ctggaaacat ctgatggttc tctagaaact gctgagggcg ggaccgcatc tggggaccat
     3421 ctgttcttgg ccctgagccg gggcaggaac tgcttaccac agatatcctg tttggcccat
     3481 attctgctgt ctctctgttc ctaaccttga tctgaacttc tctattctca gttatgtatt
     3541 ttccatgcct tgcaaaatgg cgttacttaa gctagcttgc caaacctaca ggtggggtct
     3601 ttcattcccc cctttttctg gagactaaat aaaatctttt attttatcta tggctcgtac
     3661 tctataggct tcagctggtg atattgttga gtcaaaacta gagcctggac cactgatatc
     3721 ctgtctttaa caaattggac taatcgcctc tacgccggac gcatcgtggc cggcatcacc
     3781 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
     3841 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
     3901 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcgcc gtcgttttac
     3961 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     4021 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     4081 gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     4141 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     4201 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     4261 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     4321 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     4381 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     4441 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4501 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4561 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     4621 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     4681 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     4741 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     4801 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     4861 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     4921 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     4981 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5041 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5101 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5161 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5221 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5281 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     5341 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     5401 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     5461 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5521 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5581 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5641 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5701 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5761 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5821 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     5881 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5941 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6001 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6061 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6121 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6181 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     6241 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     6301 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     6361 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     6421 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     6481 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     6541 acaattctcc ccagctcttt ctgcgctgtc cataatgaac tatttcagga gtattggagt
     6601 atgtggattc aaaacagtgg ctaattgagt caggattact taatattcga ctcctaccct
     6661 cagaatgttg ttctccaact tcaagaaact gttacccatg aaagaccccc gctgacgggt
     6721 agtcaatcac tcagaggaga ccctcccaag gaacagcgag accacaagtc ggatgcaact
     6781 gcaagagggt ttattggata cacgggtacc cgggcgacgc agtctatcgg aggactggcg
     6841 cgccgagtga ggggttgtgg gctcttttat tgagctcggg gagcagaagc gcgcgaacag
     6901 aagcgagaag cgaactgatt ggttagttca aataaggcac agggtcattt caggtccttg
     6961 gggcaccctg gaaacatctg atggttctct agaaactgct gagggcggga ccgcatctgg
     7021 ggaccatctg ttcttggccc tgagccgggg caggaactgc ttaccacaga tatcctgttt
     7081 ggcccatatt ctgctgtctc tctgttccta accttgatct gaacttctct attctcagtt
     7141 atgtattttc catgccttgc aaaatggcgt tacttaagct agcttgccaa acctacaggt
     7201 ggggtctttc attcccccct ttttctggag actaaataaa atcttttatt ttatcgatgc
     7261 atggggtcgt gcgctccttt cggtcgggcg ctgcgggtcg tggggcgggc gtcaggcacc
     7321 gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc acctcgacgt cggcggtgac
     7381 ggtgaagccg agccgctcgt agaaggggag gttgcggggc gcggaggtct ccaggaaggc
     7441 gggcaccccg gcgcgctcgg ccgcctccac tccggggagc acgacggcgc tgcccagacc
     7501 cttgccctgg tggtcgggcg agacgccgac ggtggccagg aaccacgcgg gctccttggg
     7561 ccggtgcggc gccaggaggc cttccatctg ttgctgcgcg gccagccggg aaccgctcaa
     7621 ctcggccatg cgcgggccga tctcggcgaa caccgccccc gcttcgacgc tctccggcgt
     7681 ggtccagacc gccaccgcgg cgccgtcgtc cgcgacccac accttgccga tgtcgagccc
     7741 gacgcgcgtg aggaagagtt cttgcagctc ggtgacccgc tcgatgtggc ggtccggatc
     7801 gacggtgtgg cgcgtggcgg ggtagtcggc gaacgcggcg gcgagggtgc gtacggccct
     7861 ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg tactcggtca tggtaagctt
     7921 gggctgcagg tcgaaaggcc cggagatgag gaagaggaga acagcgcggc agacgtgcgc
     7981 ttttgaagcg tgcagaatgc cgggcctccg gaggaccttc gggcgcccgc cccgccccks
     8041 mkmacttcag tctccaccca ctcagtgtgg ggaaactcca tcgcataaaa cccctccccc
     8101 caacctaaag acgacgtact ccaaaagctc gagaactaat cgaggtgcct ggacggcgcc
     8161 cggtactccg tggagtcaca tgaagcgacg gctgaggacg gaaaggccc
//
