LOCUS       sequence-380346-        8819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses murine Fosl1 (Fra1) with N-terminal tag FKBP F36V (dTAG) 
            for the dTAG system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8819)
  AUTHORS   Schneeweis C, Diersch S, Hassan Z, Krauss L, Schneider C, Lucarelli 
            D, Falcomata C, Steiger K, Ollinger R, Kramer OH, Arlt A, Grade M, 
            Schmidt-Supprian M, Hessmann E, Wirth M, Rad R, Reichert M, Saur D, 
            Schneider G
  TITLE     AP1/Fra1 confers resistance to MAPK cascade inhibition in pancreatic
            cancer.
  JOURNAL   Cell Mol Life Sci. 2022 Dec 19;80(1):12. doi: 
            10.1007/s00018-022-04638-y.
  PUBMED    36534167
REFERENCE   2  (bases 1 to 8819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Mol 
            Life Sci."; date: "2022-12-19"; volume: "80(1):12. doi"; pages: " 
            10.1007/s00018-022-04638-y"
COMMENT     Sequence Label: pLEX_305-N-dTAG-FRA1
FEATURES             Location/Qualifiers
     source          1..8819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..516
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     435..453
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(525..527,528..848)
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             855..881
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             855..881
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             885..911
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    927..951
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1776..1800)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1852..2440
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1905..1925)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2323..2334)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2512..2745
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2823..2944
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2860..2879)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2914..2933
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2984..3119
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3139..3158)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3140..3158)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3168..3185)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3168..3184)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3177..3199)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3326..3781
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3413..3432)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3623..3644
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3807..3911
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3912..3980,3981..4772)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4130..4149)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4943..5531
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5432..5451
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5685..5702
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5819..5840
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5855..5860,5861..5878,5879..5885)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5893..5909
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5898..5920
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5917..5933
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5917..5933
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5952..5972
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5954..5972
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6000..6226
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6227..6407
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6454..6579
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7072..7305
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7490..7534
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7765..7975
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7787..7983
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7834..7969
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8015..8617
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8015..8034)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8511..8531
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8660..8777
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc
       61 tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc
      121 acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg
      181 cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga
      241 caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg
      301 gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag
      361 agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg
      421 ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc
      481 tccctcgttg accgaatcac cgacctctct ccccaggggc tagcatggga gtgcaggtgg
      541 aaaccatctc cccaggagac gggcgcacct tccccaagcg cggccagacc tgcgtggtgc
      601 actacaccgg gatgcttgaa gatggaaaga aagttgattc ctcccgggac agaaacaagc
      661 cctttaagtt tatgctaggc aagcaggagg tgatccgagg ctgggaagaa ggggttgccc
      721 agatgagtgt gggtcagaga gccaaactga ctatatctcc agattatgcc tatggtgcca
      781 ctgggcaccc aggcatcatc ccaccacatg ccactctcgt cttcgatgtg gagcttctaa
      841 aactggaagg cggctacccc tacgacgtgc ccgactacgc cggctatccg tatgatgtcc
      901 cggactatgc aggcatcgat agatcaacaa gtttgtacaa aaaagcaggc tttatgtacc
      961 gagactacgg ggaaccggga ccgagctccg gggctggcag cccgtacggt cgccccgcgc
     1021 agcccccgca agctcaggca cagaccgccc agcagcagaa gttccacctt gtgccaagca
     1081 tcgacagcag cagccaggaa ctgcactgga tggtgcagcc tcatttcctg ggacccactg
     1141 gctatccccg acctctggcc tatccccagt acagtccccc tcagccccgg ccaggagtca
     1201 tacgagccct agggccacct ccgggggtgc gtcgcaggcc ctgcgagcag atcagcccag
     1261 aggaggaaga gcgccgcagg gtgagacgcg agcggaacaa gctagcagct gctaagtgca
     1321 gaaaccgaag aaaggagctg acagacttcc tgcaggcgga gaccgacaaa ttggaggatg
     1381 agaaatcggg gctgcagcga gagattgaag agctgcagaa gcagaaggaa cgccttgagc
     1441 tggtgctgga agcccatcgc cccatctgca aaatcccaga aggagacaag aaggacccag
     1501 gtggttctgg cagcaccagc ggtgctagca gcccaccagc ccccggccgc ccagtgcctt
     1561 gcatctccct ttctccagga cccgtacttg aaccggaagc actgcatacc cccacgctca
     1621 tgaccacacc ctctctgact ccttttactc cgagtctggt tttcacctat cctagcacac
     1681 cagaaccttg ctcctccgct caccgaaaga gtagcagcag cagtggcgac ccctcctccg
     1741 accccctggg ctctcctaca ctcctggctt tgtgaaccca gctttcttgt acaaagtggt
     1801 taccggttag taatgaacta gttaagtcga caatcaacgc gttaagtcga caatcaacct
     1861 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     1921 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     1981 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     2041 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     2101 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     2161 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     2221 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     2281 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     2341 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     2401 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
     2461 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     2521 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     2581 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     2641 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     2701 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt
     2761 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     2821 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     2881 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     2941 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     3001 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     3061 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     3121 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     3181 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     3241 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     3301 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     3361 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     3421 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     3481 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     3541 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     3601 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     3661 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     3721 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     3781 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     3841 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     3901 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     3961 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     4021 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     4081 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     4141 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     4201 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     4261 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     4321 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     4381 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     4441 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     4501 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     4561 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     4621 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     4681 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     4741 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     4801 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     4861 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     4921 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     4981 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5041 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     5101 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     5161 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     5221 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     5281 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     5341 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     5401 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5461 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5521 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     5581 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     5641 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     5701 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     5761 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     5821 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     5881 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     5941 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     6001 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     6061 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     6121 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     6181 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     6241 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     6301 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     6361 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     6421 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     6481 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     6541 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     6601 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     6661 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     6721 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     6781 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     6841 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     6901 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     6961 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     7021 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     7081 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     7141 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     7201 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     7261 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     7321 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     7381 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     7441 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     7501 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     7561 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     7621 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     7681 gacccacctc ccaaccccga ggggacccat gcattgcatc tcaattagtc agcaaccagg
     7741 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgcg tctcaattag
     7801 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     7861 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     7921 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     7981 aaaaagcttt ctagaggtac caccggagct taccatgacc gagtacaagc ccacggtgcg
     8041 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     8101 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     8161 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     8221 cggcgccgcc gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     8281 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     8341 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     8401 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     8461 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     8521 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     8581 cacctggtgc atgacccgca agcccggtgc cagttaacga attctagatc ttgagacaaa
     8641 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     8701 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     8761 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggcgccgg
//
