LOCUS       sequence-380379-       11258 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline-inducible expression of murine FRA1 (Fosl1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11258)
  AUTHORS   Schneeweis C, Diersch S, Hassan Z, Krauss L, Schneider C, Lucarelli 
            D, Falcomata C, Steiger K, Ollinger R, Kramer OH, Arlt A, Grade M, 
            Schmidt-Supprian M, Hessmann E, Wirth M, Rad R, Reichert M, Saur D, 
            Schneider G
  TITLE     AP1/Fra1 confers resistance to MAPK cascade inhibition in pancreatic
            cancer.
  JOURNAL   Cell Mol Life Sci. 2022 Dec 19;80(1):12. doi: 
            10.1007/s00018-022-04638-y.
  PUBMED    36534167
REFERENCE   2  (bases 1 to 11258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Mol 
            Life Sci."; date: "2022-12-19"; volume: "80(1):12. doi"; pages: " 
            10.1007/s00018-022-04638-y"
COMMENT     Sequence Label: pInducer-FRA1
FEATURES             Location/Qualifiers
     source          1..11258
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2230..2248
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2272..2290
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2314..2332
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2356..2374
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2407..2445
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2442..2466
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2575..2599
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3424..3448)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3501..3527
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        3613..4824
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4661..4680
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(4883..5500,5501..5506,5507..5542,5543..5545,5546..5581,
                     5582..5584,5585..5620,5621..5629)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5682..6255
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5849..5866)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6076..6095
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6265..6663
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             6677..7471
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6677..7471
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6731..6750)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7341..7360
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7648..8236
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7701..7721)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8119..8130)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8311..8544
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8663..8681)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8749..8853
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8854..8922,8923..9714)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9072..9091)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9885..10473
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10374..10393
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10627..10644
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10884..10905
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10920..10925,10926..10943,10944..10950)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10958..10974
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10963..10985
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10982..10998
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10982..10998
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11048..11067
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagc tcggtacccg
     2401 ggtcgaggta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt
     2461 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga
     2521 tccagcctcc gcggccccga actagtccag tgtggtggaa ttctgcagat atcaacaagt
     2581 ttgtacaaaa aagcaggctt tatgtaccga gactacgggg aaccgggacc gagctccggg
     2641 gctggcagcc cgtacggtcg ccccgcgcag cccccgcaag ctcaggcaca gaccgcccag
     2701 cagcagaagt tccaccttgt gccaagcatc gacagcagca gccaggaact gcactggatg
     2761 gtgcagcctc atttcctggg acccactggc tatccccgac ctctggccta tccccagtac
     2821 agtccccctc agccccggcc aggagtcata cgagccctag ggccacctcc gggggtgcgt
     2881 cgcaggccct gcgagcagat cagcccagag gaggaagagc gccgcagggt gagacgcgag
     2941 cggaacaagc tagcagctgc taagtgcaga aaccgaagaa aggagctgac agacttcctg
     3001 caggcggaga ccgacaaatt ggaggatgag aaatcggggc tgcagcgaga gattgaagag
     3061 ctgcagaagc agaaggaacg ccttgagctg gtgctggaag cccatcgccc catctgcaaa
     3121 atcccagaag gagacaagaa ggacccaggt ggttctggca gcaccagcgg tgctagcagc
     3181 ccaccagccc ccggccgccc agtgccttgc atctcccttt ctccaggacc cgtacttgaa
     3241 ccggaagcac tgcatacccc cacgctcatg accacaccct ctctgactcc ttttactccg
     3301 agtctggttt tcacctatcc tagcacacca gaaccttgct cctccgctca ccgaaagagt
     3361 agcagcagca gtggcgaccc ctcctccgac cccctgggct ctcctacact cctggctttg
     3421 tgaacccagc tttcttgtac aaagtggttg atatccagca cagtggcggc cgctcgagtc
     3481 tagagggccc gcggttcgaa tacccatacg acgtcccaga ctacgcttag taatgattaa
     3541 ttaaactaga aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagtaacc
     3601 cacccaagat ctggcctccg cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca
     3661 cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg tcctgatcct tccgcccgga
     3721 cgctcaggac agcggcccgc tgctcataag actcggcctt agaaccccag tatcagcaga
     3781 aggacatttt aggacgggac ttgggtgact ctagggcact ggttttcttt ccagagagcg
     3841 gaacaggcga ggaaaagtag tcccttctcg gcgattctgc ggagggatct ccgtggggcg
     3901 gtgaacgccg atgattatat aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc
     3961 cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg
     4021 ggctgctggg ctggccgggg ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg
     4081 tggagagacc gccaagggct gtagtctggg tccgcgagca aggttgccct gaactggggg
     4141 ttggggggag cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag acgcttgtga
     4201 ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg
     4261 tcttgaggcc ttcgctaatg cgggaaagct cttattcggg tgagatgggc tggggcacca
     4321 tctggggacc ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt cgtctgttgc
     4381 gggggcggca gttatggcgg tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc
     4441 cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata atgcagggtg gggccacctg
     4501 ccggtaggtg tgcggtaggc ttttctccgt cgcaggacgc agggttcggg cctagggtag
     4561 gctctcctga atcgacaggc gccggacctc tggtgagggg agggataagt gaggcgtcag
     4621 tttctttggt cggttttatg tacctatctt cttaagtagc tgaagctccg gttttgaact
     4681 atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa
     4741 tcatttgggt caatatgtaa ttttcagtgt tagactagta aattgtccgc taaattctgg
     4801 ccgtttttgg cttttttgtt agacgaagct tggtaccgag ctcggatctc caccccgtac
     4861 cggtcctgca gtcgaattca ccatgtctag actggacaag agcaaagtca taaacggagc
     4921 tctggaatta ctcaatggtg tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa
     4981 gctgggagtt gagcagccta ccctgtactg gcacgtgaag aacaagcggg ccctgctcga
     5041 tgccctgcca atcgagatgc tggacaggca tcatacccac ttctgccccc tggaaggcga
     5101 gtcatggcaa gactttctgc ggaacaacgc caagtcatac cgctgtgctc tcctctcaca
     5161 tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca gagaaacagt acgaaaccct
     5221 ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg cactgtacgc
     5281 tctgtccgcc gtgggccact ttacactggg ctgcgtattg gaggaacagg agcatcaagt
     5341 agcaaaagag gaaagagaga cacctaccac cgattctatg cccccacttc tgagacaagc
     5401 aattgagctg ttcgaccggc agggagccga acctgccttc cttttcggcc tggaactaat
     5461 catatgtggc ctggagaaac agctaaagtg cgaaagcggc gggccgaccg acgcccttga
     5521 cgattttgac ttagacatgc tcccagccga tgcccttgac gactttgacc ttgatatgct
     5581 gcctgctgac gctcttgacg attttgacct tgacatgctc cccgggtaac taagtaagga
     5641 tccgcggccg cactagagga attccgcccc tctccccccc ccccctcycc ctcccccccc
     5701 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     5761 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     5821 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     5881 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     5941 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     6001 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     6061 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     6121 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
     6181 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
     6241 aaaacacgat gataagctta aaccatggcc aagcctttgt ctcaagaaga atccaccctc
     6301 attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc
     6361 agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact
     6421 gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac
     6481 ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg
     6541 tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat
     6601 ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc
     6661 taatagccgg tccaccatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     6721 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     6781 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     6841 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     6901 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     6961 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     7021 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     7081 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     7141 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     7201 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     7261 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     7321 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     7381 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     7441 cttctatcgc cttcttgacg agttcttctg atgtacaagt aaagcggccg cgactctaga
     7501 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     7561 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgtttagtcc ctcccaattc
     7621 gatatcaagc ttatcgcgat agatcctaat caacctctgg attacaaaat ttgtgaaaga
     7681 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     7741 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     7801 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     7861 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     7921 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     7981 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     8041 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     8101 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     8161 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     8221 tgggccgcct ccccgcctga gatcctttaa gaccaatgac ttacaaggca gctgtagatc
     8281 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     8341 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     8401 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     8461 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     8521 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     8581 aacttgcaaa gaaatgaata tcagagagtg agaggcccgg gttaattaag gaaagggcta
     8641 gatcattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     8701 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     8761 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     8821 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     8881 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     8941 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     9001 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     9061 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     9121 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     9181 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     9241 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     9301 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     9361 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     9421 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     9481 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     9541 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     9601 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     9661 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     9721 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     9781 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     9841 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     9901 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     9961 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    10021 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    10081 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    10141 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    10201 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    10261 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    10321 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    10381 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    10441 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    10501 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    10561 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
    10621 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
    10681 cccgcgcgtt ggccgattca ttaatgcagc aagctcatgg ctgactaatt ttttttattt
    10741 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
    10801 ttggaggcct aggcttttgc aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa
    10861 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
    10921 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
    10981 acaggaaaca gctatgacat gattacgaat ttcacaaata aagcattttt ttcactgcat
    11041 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata
    11101 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat
    11161 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga
    11221 aattgtattt gttaaatatg tactacaaac ttagtagt
//
