LOCUS       sequence-380390-        2257 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A2 Proximal promoter sequence consisting of three times the target 
            sequence for the gRNA1 (GB1838) flanked by random sequences. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Moreno-Gimenez E, Selma S, Calvache C, Orzaez D
  TITLE     GB_SynP: A Modular dCas9-Regulated Synthetic Promoter Collection for
            Fine-Tuned Recombinant Gene Expression in Plants.
  JOURNAL   ACS Synth Biol. 2022 Sep 16;11(9):3037-3048. doi: 
            10.1021/acssynbio.2c00238. Epub 2022 Aug 31.
  PUBMED    36044643
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00238"; journalName: "ACS Synth Biol"; date: 
            "2022-09-16- 16"; volume: "11"; issue: "9"; pages: "3037-3048"
COMMENT     Sequence Label: GB3278
FEATURES             Location/Qualifiers
     source          1..2257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     290..309
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     537..555
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(559..602)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    605..626
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      835..892
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(916..933)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1087..1675)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1167..1186)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1858..1952)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1973..2257,1..375))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
       61 gagtgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
      121 ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag
      181 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt
      241 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg
      301 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt
      361 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac
      421 gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa
      481 ctcgagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
      541 ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt
      601 tctggaattc gcggccgctt ctagagcgat gagtggtctc gtgactccct cttctctcac
      661 caaccagtcc ttactgtatg agtagtaatt tgctcgagat gtcggtcctt cttctctcac
      721 caaccagtct aacgcgtcgt atctacgtca cgacgaccgt cttctctcac caaccagtca
      781 gggctagaat tactccctga gaccactcat cgcgactagt agcggccgct gcagtccggc
      841 aaaaaagggc aaggtgtcac caccctgccc tttttcttta aaaccgaaaa gattacttcg
      901 cgttatgcag gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
      961 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     1021 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     1081 ggcgtttttc cacaggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     1141 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     1201 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     1261 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     1321 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     1381 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     1441 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     1501 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     1561 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     1621 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     1681 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     1741 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     1801 ttttaaatca atctaaagta tatatgagta aacttggtct gacagctcga ggcttggatt
     1861 ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc
     1921 tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc
     1981 cgccctgcca ctcgtcgcag tactgttgta attcattaag cattctgccg acatggaagc
     2041 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
     2101 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
     2161 aatcaaaact ggtgaaactc acccagggat tggctgatac gaaaaacata ttctcaataa
     2221 accctttagg gaaataggcc aggttttcac cgtaaca
//
