LOCUS       sequence-380397-        2260 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A2 Proximal promoter sequence consisting of the target sequence for 
            the gRNA1 (GB1838) at ""b site"" (-210 from TSS). .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Moreno-Gimenez E, Selma S, Calvache C, Orzaez D
  TITLE     GB_SynP: A Modular dCas9-Regulated Synthetic Promoter Collection for
            Fine-Tuned Recombinant Gene Expression in Plants.
  JOURNAL   ACS Synth Biol. 2022 Sep 16;11(9):3037-3048. doi: 
            10.1021/acssynbio.2c00238. Epub 2022 Aug 31.
  PUBMED    36044643
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00238"; journalName: "ACS Synth Biol"; date: 
            "2022-09-16- 16"; volume: "11"; issue: "9"; pages: "3037-3048"
COMMENT     Sequence Label: GB3272
FEATURES             Location/Qualifiers
     source          1..2260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     213..232
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     460..478
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(482..525)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    528..549
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      761..818
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(842..859)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1013..1601)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1093..1112)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1784..1878)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1899..2260,1..298))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
       61 cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa
      121 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
      181 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
      241 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
      301 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
      361 tttcattatg gtgaaagttg gaacctctta cgtgcccgat caactcgagt gccacctgac
      421 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag
      481 aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcgcggccg
      541 cttctagagc gatgagtggt ctcgtgaccc atcttctctc accaaccagt ccttattgta
      601 ggcagaggca cgccctatta gtggctgcgg caaaatatct tctaagcgaa ttcctgtgct
      661 cggtggtcaa atggtatcgt ggctgcgtaa tcagccgtat ccagcaacac tacgctatct
      721 ccctgagacc actcatcgcg actagtagcg gccgctgcag tccggcaaaa aagggcaagg
      781 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt
      841 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
      901 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
      961 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccaca
     1021 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1081 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1141 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     1201 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     1261 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1321 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     1381 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     1441 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     1501 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     1561 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     1621 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     1681 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     1741 aaagtatata tgagtaaact tggtctgaca gctcgaggct tggattctca ccaataaaaa
     1801 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg
     1861 gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactcg
     1921 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
     1981 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
     2041 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
     2101 aaactcaccc agggattggc tgatacgaaa aacatattct caataaaccc tttagggaaa
     2161 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
     2221 aaatcgtcgt ggtattcact ccagagtgat gaaaacgttt
//
