LOCUS       sequence-380406-        2257 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Minimal promoter of PcPAF gene containing 62bp upstream the 
            transcription start site and the 5'UTR region (from FB029 sequence).
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Moreno-Gimenez E, Selma S, Calvache C, Orzaez D
  TITLE     GB_SynP: A Modular dCas9-Regulated Synthetic Promoter Collection for
            Fine-Tuned Recombinant Gene Expression in Plants.
  JOURNAL   ACS Synth Biol. 2022 Sep 16;11(9):3037-3048. doi: 
            10.1021/acssynbio.2c00238. Epub 2022 Aug 31.
  PUBMED    36044643
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00238"; journalName: "ACS Synth Biol"; date: 
            "2022-09-16- 16"; volume: "11"; issue: "9"; pages: "3037-3048"
COMMENT     Sequence Label: GB3413
FEATURES             Location/Qualifiers
     source          1..2257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     467..485
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(489..532)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    535..556
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      765..822
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(846..863)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1017..1605)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1097..1116)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1788..1882)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1903..2257,1..305))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
       61 agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga
      121 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
      181 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
      241 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
      301 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
      361 gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc
      421 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
      481 gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc
      541 gcggccgctt ctagagcgat gagtggtctc gtccctgtca cctttcacac agagctcatg
      601 atctggttta taaaggcggc ttcatgaccc tcaattccat atagtatcac tcccatcaca
      661 gcatttcgat atcttcaacc actttaacct tctccagagg atcatcatct caagcccttc
      721 ataaatgtga gaccactcat cgcgactagt agcggccgct gcagtccggc aaaaaagggc
      781 aaggtgtcac caccctgccc tttttcttta aaaccgaaaa gattacttcg cgttatgcag
      841 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      901 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      961 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     1021 cacaggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     1081 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     1141 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     1201 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     1261 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1321 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     1381 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     1441 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     1501 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     1561 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1621 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1681 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     1741 atctaaagta tatatgagta aacttggtct gacagctcga ggcttggatt ctcaccaata
     1801 aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt
     1861 actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca
     1921 ctcgtcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacaaac
     1981 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
     2041 gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact
     2101 ggtgaaactc acccagggat tggctgatac gaaaaacata ttctcaataa accctttagg
     2161 gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg
     2221 ccggaaatcg tcgtggtatt cactccagag tgatgaa
//
