LOCUS       sequence-380409-        2260 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A2 Proximal promoter sequence consisting of the target sequences for
            the gRNA1 (GB1838) and gRNA2 (GB1839) flanked by random sequences. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Moreno-Gimenez E, Selma S, Calvache C, Orzaez D
  TITLE     GB_SynP: A Modular dCas9-Regulated Synthetic Promoter Collection for
            Fine-Tuned Recombinant Gene Expression in Plants.
  JOURNAL   ACS Synth Biol. 2022 Sep 16;11(9):3037-3048. doi: 
            10.1021/acssynbio.2c00238. Epub 2022 Aug 31.
  PUBMED    36044643
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00238"; journalName: "ACS Synth Biol"; date: 
            "2022-09-16- 16"; volume: "11"; issue: "9"; pages: "3037-3048"
COMMENT     Sequence Label: GB3271
FEATURES             Location/Qualifiers
     source          1..2260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     219..238
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     466..484
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(488..531)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    534..555
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      767..824
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(848..865)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1019..1607)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1099..1118)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1790..1884)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1905..2260,1..304))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
       61 gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa
      121 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
      181 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
      241 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
      301 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
      361 atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaac tcgagtgcca
      421 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
      481 aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt ctggaattcg
      541 cggccgcttc tagagcgatg agtggtctcg tgacgctgta tctaatagaa tcttcggcta
      601 ttagtggctg cggcaaaata tcttctaagc gaatccgtct tctctcacca accagtctcc
      661 tgtgctcggt ggtcaaatgg tatcgtggct gcgtaatcag ccgtatccag caacactacg
      721 ctatctccct gagaccactc atcgcgacta gtagcggccg ctgcagtccg gcaaaaaagg
      781 gcaaggtgtc accaccctgc cctttttctt taaaaccgaa aagattactt cgcgttatgc
      841 aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
      901 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
      961 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1021 tccacaggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1081 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1141 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1201 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1261 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1321 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1381 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1441 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1501 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1561 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1621 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1681 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     1741 caatctaaag tatatatgag taaacttggt ctgacagctc gaggcttgga ttctcaccaa
     1801 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
     1861 ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc
     1921 cactcgtcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
     1981 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
     2041 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
     2101 ctggtgaaac tcacccaggg attggctgat acgaaaaaca tattctcaat aaacccttta
     2161 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
     2221 tgccggaaat cgtcgtggta ttcactccag agtgatgaaa
//
