LOCUS       sequence-380413-        5867 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor template for insertion of hTERT promoter (-124 C to T) driven 
            Gaussia luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Sharma S, Mukherjee AK, Roy SS, Bagri S, Lier S, Verma M, Sengupta 
            A, Kumar M, Nesse G, Pandey DP, Chowdhury S
  TITLE     Human telomerase is directly regulated by non-telomeric 
            TRF2-G-quadruplex interaction.
  JOURNAL   Cell Rep. 2021 May 18;35(7):109154. doi: 
            10.1016/j.celrep.2021.109154.
  PUBMED    34010660
REFERENCE   2  (bases 1 to 5867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-05-18"; volume: "35(7):109154. doi"; pages: " 
            10.1016/j.celrep.2021.109154"
COMMENT     Sequence Label: TERTp124_Gluc_CCR5
FEATURES             Location/Qualifiers
     source          1..5867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(9..31)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     131..150
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     344..366
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     358..375
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     359..375
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    529..540
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     regulatory      2264..2273
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2270..2827
                     /codon_start=1
                     /product="enhanced variant of secreted Gaussia luciferase"
                     /label=Gaussia-Dura Luc
                     /note="human codon-optimized"
                     /translation="MGVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADRG
                     KLPGKKLPLEVLKELEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESA
                     QGGIGEAIVDIPEIPGFKDLEPLEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQ
                     RCATFASKIQGQVDKIKGAGGD"
     CDS             2270..2827
                     /codon_start=1
                     /product="secreted Gaussia luciferase"
                     /label=hGLuc
                     /note="human codon-optimized"
                     /translation="MGVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADRG
                     KLPGKKLPLEVLKELEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESA
                     QGGIGEAIVDIPEIPGFKDLEPLEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQ
                     RCATFASKIQGQVDKIKGAGGD"
     polyA_signal    2851..2972
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2862..2881)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2916..2935
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3626..3642)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3626..3642)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3639..3661)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3650..3666
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3674..3680,3681..3698,3699..3704))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3719..3740
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3857..3874)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4028..4616)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4108..4127)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4787..5578,5579..5647))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5410..5429
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5648..5752)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5820..5838
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
       61 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
      121 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
      181 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc
      241 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
      301 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
      361 aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa tgcatctaga tgacgaccgc
      421 cggcgtttgc tgctagcttc cctgtccact agatggagag cttggctctg ttgggggaat
      481 tcatgaaagc accatctgtt taaacttcag ccttttgcag tttatcagga tgaggatgac
      541 cagcatgttg cccacaaaac caaagatgaa caccagtgag tagagcggag gcaggaggcg
      601 ggctgcgatt tgcttcacat tgattttttg gcagggctcc gatgtataat aattgatgtc
      661 atagattgga cttgacactt gataatccat cttgttccac cctgtgcata aataaaaagt
      721 gatcttttat aaagtcctag aatgtattta gttgccctcc atgaatgcaa actgttttat
      781 acatcaatag gtttttaatt gcctacatag atgtctacat tgaattaact ctctttttgg
      841 ccaagcaatg aagttttgta gtgaagggaa ggtttgctgc tagcttccct gtccactaga
      901 tggagaagct tgttaacacg cgtagatctc agcccaggga gggtgcgagg cctgttcaaa
      961 tgctagctcc ataaataaag caatttcctc cggcagtttc tgaaagtagg aaaggttaca
     1021 tttaaggttg cgtttgttag catttcagtg tttgccgacc tcagctacag catccctgca
     1081 aggcctcggg agacccagaa gtttctcgcc ccttagatcc aaacttgagc aacccggagt
     1141 ctggattcct gggaagtcct cagctgtcct gcggttgtgc cggggcccca ggtctggagg
     1201 ggaccagtgg ccgtgtggct tctactgctg ggctggaagt cgggcctcct agctctgcag
     1261 tccgaggctt ggagccaggt gcctggaccc cgaggctgcc ctccaccctg tgcgggcggg
     1321 atgtgaccag atgttggcct catctgccag acagagtgcc ggggcccagg gtcaaggccg
     1381 ttgtggctgg tgtgaggcgc ccggtgcgcg gccagcagga gcgcctggct ccatttccca
     1441 ccctttctcg acgggaccgc cccggtgggt gattaacaga tttggggtgg tttgctcatg
     1501 gtggggaccc ctcgccgcct gagaacctgc aaagagaaat gacgggcctg tgtcaaggag
     1561 cccaagtcgc ggggaagtgt tgcagggagg cactccggga ggtcccgcgt gcccgtccag
     1621 ggagcaatgc gtcctcgggt tcgtccccag ccgcgtctac gcgcctccgt cctccccttc
     1681 acgtccggca ttcgtggtgc ccggagcccg acgccccgcg tccggacctg gaggcagccc
     1741 tgggtctccg gatcaggcca gcggccaaag ggtcgccgca cgcacctgtt cccagggcct
     1801 ccacatcatg gcccctccct cgggttaccc cacagcctag gccgattcga cctctctccg
     1861 ctggggccct cgctggcgtc cctgcaccct gggagcgcga gcggcgcgcg ggcggggaag
     1921 cgcggcccag acccccgggt ccgcccggag cagctgcgct gtcggggcca ggccgggctc
     1981 ccagtggatt cgcgggcaca gacgcccagg accgcgcttc ccacgtggcg gagggactgg
     2041 ggacccgggc acccgtcctg ccccttcacc ttccagctcc gcctcctccg cgcggacccc
     2101 gccccgtccc gacccctccc gggtccccgg cccagcccct tccgggccct cccagcccct
     2161 ccccttcctt tccgcggccc cgccctctcc tcgcggcgcg agtttcaggc agcgctgcgt
     2221 cctgctgcgc acgtgggaaa gcttggtacc gagctcggat ccagccacca tgggagtcaa
     2281 agttctgttt gccctgatct gcatcgctgt ggccgaggcc aagcccaccg agaacaacga
     2341 agacttcaac atcgtggccg tggccagcaa cttcgcgacc acggatctcg atgctgaccg
     2401 cgggaagttg cccggcaaga agctgccgct ggaggtgctc aaagagctgg aagccaatgc
     2461 ccggaaagct ggctgcacca ggggctgtct gatctgcctg tcccacatca agtgcacgcc
     2521 caagatgaag aagttcatcc caggacgctg ccacacctac gaaggcgaca aagagtccgc
     2581 acagggcggc ataggcgagg cgatcgtcga cattcctgag attcctgggt tcaaggactt
     2641 ggagcccctg gagcagttca tcgcacaggt cgatctgtgt gtggactgca caactggctg
     2701 cctcaaaggg cttgccaacg tgcagtgttc tgacctgctc aagaagtggc tgccgcaacg
     2761 ctgtgcgacc tttgccagca agatccaggg ccaggtggac aagatcaagg gggccggtgg
     2821 tgactaagcg gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca
     2881 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     2941 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     3001 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     3061 gtactcgaga cgcgtgctct gttgggggaa ttcatgaaag caccatctca ccaaataaaa
     3121 tcttgtgctc tatagcacca tggagtgaat gaagctttga caacaatttt aagaaactgg
     3181 gtcaagcatg gtgcagctca gcctttttgt tgttgttgtt gttgttgtga gacagggtct
     3241 cactctgtcg cccaggctgg agtgcagtgg cacgatcatg gctcactgca gcctggacca
     3301 tcccgggctc aagcaatcct ctcacctcac ccctccaagt ggctggggac tacaggcgcc
     3361 accacaccaa gctaattttg tttattttta tagatacaag atcacactat gttgcccagg
     3421 ctggtctcga actcctgggc tcaagcgatc ctcctgcctc agcctcctag aatgcattta
     3481 aattttgctg ctagcttccc tgtccactag atggagagct tggctctgtt gggggaattc
     3541 atgaaagcac catctgcggc cgcgtcgtat cggatcccgg gcccgtcgac tgcagaggcc
     3601 tgcatgcaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     3661 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     3721 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     3781 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     3841 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     3901 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     3961 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     4021 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     4081 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     4141 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     4201 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     4261 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     4321 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     4381 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     4441 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     4501 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     4561 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     4621 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     4681 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     4741 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     4801 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     4861 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     4921 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     4981 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     5041 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     5101 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     5161 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     5221 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     5281 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     5341 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     5401 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     5461 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     5521 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     5581 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     5641 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     5701 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     5761 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     5821 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgac
//
