LOCUS       sequence-380421-        2194 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Minimal promoter of slANS gene (Solyc10g076660) containing 62bp 
            upstream the transcription start site and the 5'UTR region. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Moreno-Gimenez E, Selma S, Calvache C, Orzaez D
  TITLE     GB_SynP: A Modular dCas9-Regulated Synthetic Promoter Collection for
            Fine-Tuned Recombinant Gene Expression in Plants.
  JOURNAL   ACS Synth Biol. 2022 Sep 16;11(9):3037-3048. doi: 
            10.1021/acssynbio.2c00238. Epub 2022 Aug 31.
  PUBMED    36044643
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00238"; journalName: "ACS Synth Biol"; date: 
            "2022-09-16- 16"; volume: "11"; issue: "9"; pages: "3037-3048"
COMMENT     Sequence Label: GB3409
FEATURES             Location/Qualifiers
     source          1..2194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     178..197
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     425..443
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(447..490)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    493..514
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      660..717
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(741..758)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(912..1500)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(992..1011)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1683..1777)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1798..2194,1..263))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca
       61 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
      121 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
      181 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
      241 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca
      301 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc
      361 ccgatcaact cgagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
      421 taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa
      481 ggatgatttc tggaattcgc ggccgcttct agagcgatga gtggtctcgt ccctatcttg
      541 tatgagataa atttcaagtg ctataaatat acctaacgaa aagttatagt tagcagtact
      601 gaaaaaaacg agataaacaa tgtgagacca ctcatcgcga ctagtagcgg ccgctgcagt
      661 ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta
      721 cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      781 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      841 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      901 ttgctggcgt ttttccacag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      961 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     1021 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     1081 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     1141 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1201 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     1261 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     1321 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     1381 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     1441 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     1501 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     1561 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     1621 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ctcgaggctt
     1681 ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
     1741 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta
     1801 cgccccgccc tgccactcgt cgcagtactg ttgtaattca ttaagcattc tgccgacatg
     1861 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
     1921 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
     1981 gtttaaatca aaactggtga aactcaccca gggattggct gatacgaaaa acatattctc
     2041 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
     2101 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagtgatg aaaacgtttc
     2161 agtttgctca tggaaaacgg tgtaacaagg gtga
//
