LOCUS       sequence-380422-        2233 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Minimal promoter of At2S3 gene containing 84bp upstream the 
            transcription start site and the 5'UTR region (from GB0029 
            sequence). .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Moreno-Gimenez E, Selma S, Calvache C, Orzaez D
  TITLE     GB_SynP: A Modular dCas9-Regulated Synthetic Promoter Collection for
            Fine-Tuned Recombinant Gene Expression in Plants.
  JOURNAL   ACS Synth Biol. 2022 Sep 16;11(9):3037-3048. doi: 
            10.1021/acssynbio.2c00238. Epub 2022 Aug 31.
  PUBMED    36044643
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00238"; journalName: "ACS Synth Biol"; date: 
            "2022-09-16- 16"; volume: "11"; issue: "9"; pages: "3037-3048"
COMMENT     Sequence Label: GB3411
FEATURES             Location/Qualifiers
     source          1..2233
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     195..214
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     442..460
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(464..507)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    510..531
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      716..773
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(797..814)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(968..1556)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1048..1067)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1739..1833)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1854..2233,1..280))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
       61 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
      121 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
      181 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
      241 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
      301 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
      361 tggaacctct tacgtgcccg atcaactcga gtgccacctg acgtctaaga aaccattatt
      421 atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa
      481 tccttagctt tcgctaagga tgatttctgg aattcgcggc cgcttctaga gcgatgagtg
      541 gtctcgtccc gcatgcatgc attcttacac gtgattgcca tgcaaatctc ctttctcacc
      601 tataaataca aaccaaccct tcactacact cttcactcaa accaaaacaa gaaaacatac
      661 acaaatagca aaacaatgtg agaccactca tcgcgactag tagcggccgc tgcagtccgg
      721 caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa agattacttc
      781 gcgttatgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      841 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
      901 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      961 tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     1021 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1081 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1141 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1201 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1261 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1321 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1381 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     1441 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     1501 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     1561 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     1621 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     1681 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagctcg aggcttggat
     1741 tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt
     1801 ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc
     1861 ccgccctgcc actcgtcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
     1921 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
     1981 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
     2041 aaatcaaaac tggtgaaact cacccaggga ttggctgata cgaaaaacat attctcaata
     2101 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
     2161 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gtgatgaaaa cgtttcagtt
     2221 tgctcatgga aaa
//
