LOCUS       sequence-380423-        3347 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Random sequence R3 of 1240 bp for A1 distal promoter position. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Moreno-Gimenez E, Selma S, Calvache C, Orzaez D
  TITLE     GB_SynP: A Modular dCas9-Regulated Synthetic Promoter Collection for
            Fine-Tuned Recombinant Gene Expression in Plants.
  JOURNAL   ACS Synth Biol. 2022 Sep 16;11(9):3037-3048. doi: 
            10.1021/acssynbio.2c00238. Epub 2022 Aug 31.
  PUBMED    36044643
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00238"; journalName: "ACS Synth Biol"; date: 
            "2022-09-16- 16"; volume: "11"; issue: "9"; pages: "3037-3048"
COMMENT     Sequence Label: GB3270
FEATURES             Location/Qualifiers
     source          1..3347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      34..91
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(115..132)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(286..874)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(366..385)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1057..1151)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1172..1831)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1746..1765
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1993..2011
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2015..2058)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2061..2082
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 accactcatc gcgactagta gcggccgctg cagtccggca aaaaagggca aggtgtcacc
       61 accctgccct ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct
      121 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      181 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      241 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg
      301 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
      361 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
      421 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
      481 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
      541 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
      601 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
      661 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
      721 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
      781 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
      841 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
      901 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
      961 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     1021 atatgagtaa acttggtctg acagctcgag gcttggattc tcaccaataa aaaacgcccg
     1081 gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat
     1141 caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcgtcgcagt
     1201 actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa
     1261 cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga
     1321 aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca
     1381 cccagggatt ggctgatacg aaaaacatat tctcaataaa ccctttaggg aaataggcca
     1441 ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt
     1501 cgtggtattc actccagagt gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac
     1561 aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg
     1621 gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat
     1681 ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac
     1741 attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa
     1801 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
     1861 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
     1921 ttacgtgccc gatcaactcg agtgccacct gacgtctaag aaaccattat tatcatgaca
     1981 ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct
     2041 ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agcgatgagt ggtctcggga
     2101 gccctgattt tttcactact aatagtactc acggcgcaat accagcacag cctagtctcg
     2161 ccagaatgct ggtcagcata cgaaagagct taaggcaggc caattccact gtcagggtca
     2221 cttgggtgtt tagcactacc gacaggtacg ctagtatgcg ttcttcctac cagaggtctg
     2281 tggccgcgtg gtcaaaagtg cggctttcgt atttgctgct cgtgtttact ctcacaaact
     2341 tgacctgcac gccaaagaga tgcttcttgt ggaactcgac aacgcaacaa cgcgacggat
     2401 ctacgtcaca gcgtgcatag tgaaaacgga gttgctgacg acgaaagcga cattgggatc
     2461 tgtcagttgt cattcgcgaa aaacatccgt ccccgaggcg gacactgatt gacgcggttt
     2521 tgtagaaggt taggggaata ggttagattg agtggcttaa gaatgtaaaa tctgggatta
     2581 tagtgtagta atctctgatt aacggtgacg gttttaagac aggtgttcgc aaaatcaagc
     2641 ggggtcattt caacagatat tgctgatggt ttaggcgtac aatgccctga agaataatta
     2701 agaaaaaagc acccctcgtc gcctagaatt acctaccgcg gtccaccata ccttcgatta
     2761 tcgcgcccac tctcccatta gtcggcagag gtggttgtgt tgcgatagcc cagtatgata
     2821 ttctaaggcg ttacgctgat gaatattcta cagagttgcc ataggcgttg aacgctacac
     2881 ggacgatacg aatttacgta tagagcgggt catcgaaagg ttatactctc gtagttaaca
     2941 tctagcccgg ccctatcagt acagcagtgc cttgaatgac atactcatca ttaaattttc
     3001 tctacagcca aacgaccaag tgcatttcca gggagcgcga tggagattca ttctctcgcc
     3061 agcactgtaa taggcactaa aagagtgatg ataatcatga gtgccgcgct aaggtggtgt
     3121 cggaacaaag cggtcttacg gtcagtcgta ttccttctcg agttccgtcc agttgagcgt
     3181 gtcactccca gtgtacctgc aagccgagat ggctgtgctt ggagtcaatc gcatgtagca
     3241 tggtcaccag acaccggggc accagttttc acgcctaaag cataaacgac gagcagtcat
     3301 gaaagtctta gtactggacg tgccgtttca ctgcgaatat gactgag
//
