LOCUS       sequence-38043-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Subach FV, Patterson GH, Renz M, Lippincott-Schwartz J, Verkhusha VV
  TITLE     Bright monomeric photoactivatable red fluorescent protein for 
            two-color super-resolution sptPALM of live cells.
  JOURNAL   J Am Chem Soc. 2010 May 12;132(18):6481-91.
  PUBMED    20394363
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc."; date: "2010-05-12"; volume: "132(18)"; pages: "6481-91"
COMMENT     Sequence Label: pTfR-PATagRFP
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(128..841)
                     /codon_start=1
                     /product="monomeric photoactivatable red fluorescent 
                     protein (Gunewardene et al., 2011)"
                     /label=PAmKate
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSSTFINHTQGIPDFWKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMKKKTLGWEPSTEKLKPADGGLEGRVD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDRRLEIIKEADKETYWEQHEVAV
                     ARYSDLPSKLGHKLN"
     regulatory      838..847
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
       61 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat ctagagtcgc
      121 ggccgcttta attaagcttg tgccccagtt tgctagggag gtcggagtat ctggccacag
      181 ccacctcgtg ctgctcccag taggtctctt tgtcggcctc cttgatgatt tccagtctgc
      241 ggtccacata gtagacgccg ggcatcttga ggttcttagc gggtttcttg gatctgtatg
      301 tggtcttgaa gttgcagatc aggtggcccc cgcccacgag cttcagggcc atgtcgaccc
      361 tgccttccag gccgccgtca gcgggtttca gtttctcggt gctcggttcc cagccgagtg
      421 ttttcttctt catcacaggg ccgttggatg ggaagttcac ccctctgatc ttgacgttgt
      481 agatgaggca gccgtcctgg aggctggtgt cctgggtagc ggtcagcacg cccccgtctt
      541 cgtatgtggt gactctctcc catgtgaagc cctcagggaa ggactgcttc cagaagtcgg
      601 ggatgccctg ggtgtggttg atgaaggtgc tgctgccgta catgaagctg gtagccagga
      661 tgtcgaaggc gaaggggaga gggccgccct cgaccacctt gattctcatg gtctgggtgc
      721 cctcgtaggg cttgccttcg ccctcggatg tgcacttgaa gtggtggttg ttcacggtgc
      781 cctccatgta cagcttcatg tgcatgttct ccttaatcag ctcttcgccc ttagacacca
      841 tggtggcgac
//
