LOCUS       sequence-38044-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Subach FV, Patterson GH, Renz M, Lippincott-Schwartz J, Verkhusha VV
  TITLE     Bright monomeric photoactivatable red fluorescent protein for 
            two-color super-resolution sptPALM of live cells.
  JOURNAL   J Am Chem Soc. 2010 May 12;132(18):6481-91.
  PUBMED    20394363
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc."; date: "2010-05-12"; volume: "132(18)"; pages: "6481-91"
COMMENT     Sequence Label: pVSVG-PATagRFP
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(125..838)
                     /codon_start=1
                     /product="monomeric photoactivatable red fluorescent 
                     protein (Gunewardene et al., 2011)"
                     /label=PAmKate
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSSTFINHTQGIPDFWKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMKKKTLGWEPSTEKLKPADGGLEGRVD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDRRLEIIKEADKETYWEQHEVAV
                     ARYSDLPSKLGHKLN"
     regulatory      835..844
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
       61 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcta gagtcgcggc
      121 cgctttaatt aagcttgtgc cccagtttgc tagggaggtc ggagtatctg gccacagcca
      181 cctcgtgctg ctcccagtag gtctctttgt cggcctcctt gatgatttcc agtctgcggt
      241 ccacatagta gacgccgggc atcttgaggt tcttagcggg tttcttggat ctgtatgtgg
      301 tcttgaagtt gcagatcagg tggcccccgc ccacgagctt cagggccatg tcgaccctgc
      361 cttccaggcc gccgtcagcg ggtttcagtt tctcggtgct cggttcccag ccgagtgttt
      421 tcttcttcat cacagggccg ttggatggga agttcacccc tctgatcttg acgttgtaga
      481 tgaggcagcc gtcctggagg ctggtgtcct gggtagcggt cagcacgccc ccgtcttcgt
      541 atgtggtgac tctctcccat gtgaagccct cagggaagga ctgcttccag aagtcgggga
      601 tgccctgggt gtggttgatg aaggtgctgc tgccgtacat gaagctggta gccaggatgt
      661 cgaaggcgaa ggggagaggg ccgccctcga ccaccttgat tctcatggtc tgggtgccct
      721 cgtagggctt gccttcgccc tcggatgtgc acttgaagtg gtggttgttc acggtgccct
      781 ccatgtacag cttcatgtgc atgttctcct taatcagctc ttcgccctta gacaccatgg
      841 tggcgaccgg tggatccgcg ccggtggagc cggtgga
//
