LOCUS       sequence-380448-        3348 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Random sequence R2 of 1240 bp for A1 distal promoter position. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Moreno-Gimenez E, Selma S, Calvache C, Orzaez D
  TITLE     GB_SynP: A Modular dCas9-Regulated Synthetic Promoter Collection for
            Fine-Tuned Recombinant Gene Expression in Plants.
  JOURNAL   ACS Synth Biol. 2022 Sep 16;11(9):3037-3048. doi: 
            10.1021/acssynbio.2c00238. Epub 2022 Aug 31.
  PUBMED    36044643
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00238"; journalName: "ACS Synth Biol"; date: 
            "2022-09-16- 16"; volume: "11"; issue: "9"; pages: "3037-3048"
COMMENT     Sequence Label: GB3269
FEATURES             Location/Qualifiers
     source          1..3348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      34..91
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(115..132)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(286..874)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(366..385)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1057..1151)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1172..1831)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1746..1765
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1993..2011
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2015..2058)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2061..2082
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 accactcatc gcgactagta gcggccgctg cagtccggca aaaaagggca aggtgtcacc
       61 accctgccct ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct
      121 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      181 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      241 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg
      301 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
      361 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
      421 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
      481 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
      541 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
      601 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
      661 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
      721 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
      781 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
      841 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
      901 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
      961 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     1021 atatgagtaa acttggtctg acagctcgag gcttggattc tcaccaataa aaaacgcccg
     1081 gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat
     1141 caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcgtcgcagt
     1201 actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa
     1261 cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga
     1321 aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca
     1381 cccagggatt ggctgatacg aaaaacatat tctcaataaa ccctttaggg aaataggcca
     1441 ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt
     1501 cgtggtattc actccagagt gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac
     1561 aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg
     1621 gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat
     1681 ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac
     1741 attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa
     1801 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
     1861 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
     1921 ttacgtgccc gatcaactcg agtgccacct gacgtctaag aaaccattat tatcatgaca
     1981 ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct
     2041 ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agcgatgagt ggtctcggga
     2101 gctaactgag atactaccat agatgactac ccatccctct aggccttaga taaccggata
     2161 cagtgacttt gataggtttg tggggtacag ctatgacttg catagctgcg tatggaggaa
     2221 ggaacttttg cgtgttagta tgttgacccg tgtattacgc atgcgggtag atcatgtggg
     2281 tagagacatc caggtcaagt tctcgacctt ctcgtgggag gtgatccagt tcactatagg
     2341 accattccat tcgagcatgg cactaagtac gccgtcccca ttctggtaat catcatccct
     2401 atcagggttt ggagtgagtg gtgacggtta ttccccagga atggacttct tattcacagt
     2461 cggtcacatt gggctactcc ttgggtcttc cgcttggccc ggtctgttgg gccgccattg
     2521 cgtgagtttc ggccctgtgc tgcgctgtgt cggcgattct cattggggcc tcacatctgg
     2581 aaaccccgac ctattttgac ggcaccattg gcggaagttg ctgggcttgt gcaccgtgaa
     2641 gtcctccgcg ggcctccccc ctacaaaaga cgataagctc cggcaagcag cattgatcaa
     2701 cgcaaggatc ggcgatatta acagagatac ggctgattac tcttgttggt gtggtatcgc
     2761 taaactgcgc cgcggagcct tatggcaaag tcgtccgcga accattccgg tagcgcttaa
     2821 ggtccatagc acattcatcg catcccggcg tgcgttcaat ttgacgaccc cttggcgcaa
     2881 aggtgctggc cacgtgctaa attaaagcgg ctgcactgct gtaaggtccg ttacggagag
     2941 ggcggcctgg ggagagcact agcccatcga cctgtacagg aacactctat attgttctcg
     3001 gacggacaga ttactagagt gccgctttca gcccccctgt cgtcgccgac gtctgtaata
     3061 tggcgttgtt gtgattccac tctattgagg cattaactga tgcgtaagga gatctggaat
     3121 gaactggcct atgtcactga aactgtgcaa acacccaatg tcgttagtgt aggttctgac
     3181 cgatacctgc ttcgttgaga actcacaatt ttacaactgg ggacataatc cctacgccca
     3241 tcatctactc gcgtccctgt ggctccagtt catgtaatgg gagagtatcc tccacaagat
     3301 ctagtgctat ggtggtatag taagctcgta ctgtggtaga tgactgag
//
