LOCUS       sequence-38046-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Subach FV, Patterson GH, Manley S, Gillette JM, Lippincott-Schwartz 
            J, Verkhusha VV
  TITLE     Photoactivatable mCherry for high-resolution two-color fluorescence 
            microscopy.
  JOURNAL   Nat Methods. 2009 Feb;6(2):153-9. Epub 2009 Jan 25.
  PUBMED    19169259
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2009-02"; volume: "6(2)"; pages: "153-9. Epub 2009
            Jan 25"
COMMENT     Sequence Label: pPAmCherry-b-actin
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             19..726
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(164..182)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ccgctagcgg tcgccaccat ggtgagcaag ggcgaggagg ataacatggc catcattaag
       61 gagttcatgc gcttcaaggt gcacatggag gggtccgtga acggccacgt gttcgagatc
      121 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
      181 aagggcggcc ccctgccctt cacctgggac atcctgagcc ctcagttcat gtacggctcc
      241 aatgcctacg tgaagcaccc cgccgacatc cccgactact ttaagctgtc cttccccgag
      301 ggcttcaagt gggagcgcgt gatgaaattc gaggacggcg gcgtggtgac cgtgacccag
      361 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
      421 ccctccgacg gccccgtgat gcagaagaag accatgggct gggaggccct ctccgagcgg
      481 atgtaccccg aggacggcgc cctgaagggc gaggtcaagc ccagagtgaa gctgaaggac
      541 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
      601 cccggcgcct acaacgtcaa ccgcaagctg gacatcacca gccacaacga ggactacacc
      661 atcgtggagc agtacgagag agccgagggc cgccactcca ccggcggcat ggacgagctg
      721 tacaagtccg gactcagatc tcgagctatg gatgatgata tcgccgcgct cgtcgtcgac
      781 aacggctccg gcatgtgc
//
