LOCUS       sequence-380514-        4685 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mouse Etnppl sequence is inserted in pGEM-T Easy vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Tsujioka H, Yamashita T
  TITLE     Utilization of ethanolamine phosphate phospholyase as a unique 
            astrocytic marker.
  JOURNAL   Front Cell Neurosci. 2023 Jan 30;17:1097512. doi: 
            10.3389/fncel.2023.1097512. eCollection 2023.
  PUBMED    36794261
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Neurosci."; date: "2023-01-30"; volume: "17:1097512. doi"; pages: " 
            10.3389/fncel.2023.1097512. eCollection 2023"
COMMENT     Sequence Label: etnppl-pGEMtEasy
FEATURES             Location/Qualifiers
     source          1..4685
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(23..40)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(194..782)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(274..293)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(953..1744,1745..1813))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1576..1595
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1814..1918)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1996..2451)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2133..2154)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2345..2364
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2577..2599
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2591..2608
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2592..2608
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2615..2634
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2615..2633
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2664..2673
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2668..2677
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4441..4459)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4442..4459)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4477..4493)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4477..4493)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4490..4512)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4501..4517
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4525..4531,4532..4549,4550..4555))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4570..4591
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
       61 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      121 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      181 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      241 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      301 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      361 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      421 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      481 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      541 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
      601 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
      661 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
      721 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
      781 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
      841 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
      901 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
      961 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     1021 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     1081 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     1141 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     1201 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     1261 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     1321 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     1381 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     1441 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     1501 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     1561 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     1621 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     1681 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     1741 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     1801 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     1861 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     1921 catttccccg aaaagtgcca cctgatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     1981 aaataccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     2041 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     2101 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     2161 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     2221 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     2281 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     2341 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     2401 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc
     2461 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     2521 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     2581 gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat agggcgaatt
     2641 gggcccgacg tcgcatgctc ccggccgcca tggcggccgc gggaattcga ttagtgacct
     2701 ccgataccca gggcaggagg cacaggaggg gagaaaaaaa atcctttgca gcgtcccagg
     2761 ttacagaggg aggaacattc attgacttga agggtgcagc ggtcatgtgc gagctctata
     2821 gcaagcagga caccctggcg ctgagggaga ggcacatcgg gccctcgtgc aaaattttct
     2881 ttgccgcgga tcccatcaaa ataatgcgag cccaggggca gtacatgttt gatgagaaag
     2941 gcgaacggta cttagactgc atcaacaacg tggcgcatgt gggacactgt cacccagaag
     3001 tggtcaaagc tgccgcgaaa cagatggaat tactaaatac aaattctcgc ttcctccacg
     3061 acaacatcat tgagtttgcc aagcgcctta ctgccaccct gcctcaggag ctctccgttt
     3121 gctacttcac aaactccgga tccgaagcca atgacttagc cttacgtctg gcacggcagt
     3181 tccgaggcca ccaggatgtg atcacccttg accatgctta ccacggccac ctgtcgtcat
     3241 taattgagat cagtccttat aagtttcaga agggcaaaga tgtcaagagg gaaactgtac
     3301 atgtggcacc agctccagac acttacagag ggaaatacag agaggaccac gaagacccat
     3361 ccactgcata tgccgacgag gtgaagaaga tcattgaaga ggctcacagc agcggaagga
     3421 agattgctgc ctttattgct gaatccatgc agagttgtgg tggacaaatt attcctccag
     3481 caggctactt ccagaaagtg gctgaacaca ttcacaaagc aggaggtgtg ttcattgctg
     3541 acgaagttca agtaggcttt ggcagagttg ggaggtactt ctggagcttc cagatgtatg
     3601 gtgaagactt cgttccagac atcgtcacca tggggaaacc tatgggcaac ggtcacccga
     3661 tatcctgtgt ggtgacaacc aaagaaattg cagaagcctt cagcagctcg ggcatggaat
     3721 atttcaatac gtatggagga aatccagtgt cttgtgcagt tggcttggct gtgctggatg
     3781 taattgaaaa ggagaacctt caagggaatg ctgtcagagt ggggacctat ctcatggagc
     3841 tgctgagtga acagaaggct aagcatccct tgatagggga catcagaggt gtcggccttt
     3901 ttatcggcat cgatttggtg aaggaccgtg agaaaagaac acctgcgaca gctgaagcac
     3961 agcacatcat ctacgagatg aaaggaaagg gggtacttct cagtgccgat ggccctcaca
     4021 ggaacgtgct gaaaataaaa ccacccatgt gcttcacgga agacgatgca aagttcctgg
     4081 tggaccacct tgacggcatt ttaacagttt tagaagaagc catggactcc aagagtggaa
     4141 ctgtgttctc tgagaacaca gcttacagaa caaagatgcc taaggaaata caagtggaat
     4201 tgcccaacct tagtgccacc gaggccagag aaatccccag gggaaagaga aatggtgtat
     4261 gctcagacca gcaagccctg ctcagcaaaa gactcaagac atgagagctg agtgctaaac
     4321 agtggataaa tatccaggaa cacagtgtgc aagtggatgc aatcactagt gaattcgcgg
     4381 ccgcctgcag gtcgaccata tgggagagct cccaacgcgt tggatgcata gcttgagtat
     4441 tctatagtgt cacctaaata gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     4501 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     4561 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     4621 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     4681 tttgc
//
