LOCUS       sequence-38052-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  TITLE     Linda Hicke lab unpublished Ubiquitin constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: LHP599 - pACT2-Ub
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..62
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             42..68
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             90..317
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             90..317
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
ORIGIN
        1 ccccaccaaa cccaaaaaaa gagatctcta tggcttaccc atacgatgtt ccagattacg
       61 ctagcttggg tggtcatatg gccatggcca tgcagatctt cgtcaagacg ttaaccggta
      121 aaaccataac tctagaagtt gaatcttccg ataccatcga caacgttaag tcgaaaattc
      181 aagacaagga aggcattcca cctgatcaac aaagattgat ctttgccggt aagcagctcg
      241 aggacggtag aacgctgtct gattacaaca ttcagaagga gtcgacctta catcttgtct
      301 taagactaag aggtggttga ggatccgttt tatcagttgc ccacacctac gagttcaaaa
      361 aagaattcga gctcgagaga tctatgaatc gtagatactg aaaaaccccg caagttcact
      421 tcaactgtgc atcgtgcacc atctcaattt ctttcattta tacatcgttt tgccttcttt
      481 tatgtaacta tactcctcta agtttcaatc ttggccatgt aacctctgat ctatagaatt
      541 ttttaaatga ctagaattaa tgcccatctt ttttttggac ctaaattctt catgaaaata
      601 tattacgagg gcttattcag aagctttgga cttcttcgcc agaggtttgg tcaagtctcc
      661 aatcaaggtt gtcggcttgt ctaccttgcc agaaatttac gaaaagatgg aaaagggtca
      721 aatcgttggt agatacgttg ttgacacttc taaataagcg aatttcttat gatttatgat
      781 tttatataat agtataaaaa
//
