LOCUS       sequence-380564-       10404 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for SLP76/VISH2 pYtag biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10404)
  AUTHORS   Farahani PE, Yang X, Mesev EV, Fomby KA, Brumbaugh-Reed EH, Bashor 
            CJ, Nelson CM, Toettcher JE
  TITLE     pYtags enable spatiotemporal measurements of receptor tyrosine 
            kinase signaling in living cells.
  JOURNAL   eLife. 2023 May 22;12:e82863. doi: 10.7554/eLife.82863.
  PUBMED    37212240
REFERENCE   2  (bases 1 to 10404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2023-05-22"; volume: "12:e82863. doi"; pages: " 
            10.7554/eLife.82863"
COMMENT     Sequence Label: pHR Clover-VISH2
FEATURES             Location/Qualifiers
     source          1..10404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             330..374
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    659..776
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        929..1336
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      1406..1415
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1457..1478)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     2065..2086
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2877..3465
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2930..2950)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3348..3359)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3468..3484)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3469..3485)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3575..3808
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3833..3851)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3834..3851)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4141..4160)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4374..4393)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4493..4515
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4553..4571)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4639..4743
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4744..4812,4813..5604)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4962..4981)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5775..6363
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6264..6283
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6517..6534
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6604..6624)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6609..6938
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6789..6924
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8072..8137
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8267..8287
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8712..8846
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8749..8768)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8803..8822
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9014..9647
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9694..9819
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    join(10316..10404,1..145)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
       61 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
      121 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
      181 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
      241 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
      301 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
      361 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
      421 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
      481 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
      541 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
      601 ccattcgatt agtgaacgga tctcgacggt atcgccaaat ggcagtattc atccacaatt
      661 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
      721 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
      781 tttattacag ggacagcaga gatccagttt ggatcgataa gcttgatatc gaattcctgc
      841 agccccgata aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc
      901 cacctgtagg tttggcaagc tagctgcagt aacgccattt tgcaaggcat ggaaaaatac
      961 caaaccaaga atagagaagt tcagatcaag ggcgggtaca tgaaaatagc taacgttggg
     1021 ccaaacagga tatctgcggt gagcagtttc ggccccggcc cggggccaag aacagatggt
     1081 caccgcagtt tcggccccgg cccgaggcca agaacagatg gtccccagat atggcccaac
     1141 cctcagcagt ttcttaagac ccatcagatg tttccaggct cccccaagga cctgaaatga
     1201 ccctgcgcct tatttgaatt aaccaatcag cctgcttctc gcttctgttc gcgcgcttct
     1261 gcttcccgag ctctataaaa gagctcacaa cccctcactc ggcgcgccag tcctccgaca
     1321 gactgagtcg cccggggggg atctggagct ctcgagaatt ctcacgcgtc aagtggagca
     1381 aggcaggtgg acagtggatc cggtcgccac catggtgagc aagggcgagg agctgttcac
     1441 cggggttgtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     1501 ccgcggcgag ggcgagggcg atgccaccaa cggcaagctg accctgaagt tcatctgcac
     1561 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcgtggc
     1621 ctgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     1681 cgaaggctac gtccaggagc gcaccatctc tttcaaggac gacggtacct acaagacccg
     1741 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     1801 cttcaaggag gacggcaaca tcctggggca caagctggag tacaacttca acagccacaa
     1861 cgtctatatc acggccgaca agcagaagaa cggcatcaag gctaacttca agatccgcca
     1921 caacgttgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     1981 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc catcagtccg ccctgagcaa
     2041 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccggtag
     2101 tggctcctgg tacgcaggcc ctatggaacg ggctggcgca gaatctatcc tggccaaccg
     2161 atctgatggg acgttcttgg tacggcaacg ggttaaagat gccgccgagt tcgcgatctc
     2221 cattaagtat aacgtggaag ttaagcacat aaaaattatg actgcagagg gactttatcg
     2281 aatcacggag aagaaggctt tccgcgggct taccgagctt gtggagtttt accagcaaaa
     2341 tagcttgaaa gattgtttta agtccctcga cacaaccctc cagttcccat ttggcagcgg
     2401 gtcaggctct gggtcagggt cagggtccag cggtagtggc agcggtggag gtggaggtag
     2461 cggaggtggc tctgggggta gttctggttc ttcaagcggc ggcggtggta gctggtataa
     2521 taaatccatc agcagagata aggctgaaaa acttctcctg gatactggaa aggagggcgc
     2581 ttttatggtt cgggacagtc gcaccgcagg aacctacact gtaagcgttt tcacgaaagc
     2641 tgtagtatct gaaaacaatc cgtgcatcaa gcattatcat attaaagaga ctaatgataa
     2701 ccccaagcgg tattatgtag cagagaagta cgtgttcgat agtattccct tgcttattaa
     2761 ttatcatcag cacaatgggg gaggccttgt tacgcggctc aggtatccag tctgactgag
     2821 gccgcgactc tagagtcgac ctgcaggcat gcaagcttga tatcaagctt atcgataatc
     2881 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     2941 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     3001 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     3061 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     3121 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     3181 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     3241 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     3301 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     3361 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     3421 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
     3481 ctcgagggaa ttaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta
     3541 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     3601 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     3661 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     3721 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     3781 ttttagtcag tgtggaaaat ctctagcagc atctagaatt aattccgtgt attctatagt
     3841 gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta
     3901 acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag accctatcat
     3961 ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga gtttctggta
     4021 acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc gctagccaga
     4081 tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg
     4141 cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg
     4201 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct
     4261 ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct
     4321 gcttcctaat gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc
     4381 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     4441 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     4501 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     4561 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     4621 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     4681 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     4741 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     4801 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     4861 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     4921 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     4981 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     5041 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     5101 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     5161 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     5221 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     5281 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     5341 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     5401 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     5461 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     5521 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     5581 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     5641 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     5701 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     5761 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     5821 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     5881 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     5941 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6001 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6061 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6121 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6181 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6241 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6301 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6361 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6421 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     6481 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     6541 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     6601 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     6661 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     6721 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     6781 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     6841 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     6901 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct
     6961 tgcgagatat gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa
     7021 gacgcggact catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa
     7081 cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg
     7141 agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc
     7201 cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac
     7261 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
     7321 gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg
     7381 aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg
     7441 aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg
     7501 ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg
     7561 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg
     7621 gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct
     7681 gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct
     7741 gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc
     7801 cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg
     7861 cgcggcattg acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc
     7921 gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt
     7981 tatgagtggg ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga
     8041 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg
     8101 tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga
     8161 atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc
     8221 atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag
     8281 aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt
     8341 gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact
     8401 gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta
     8461 taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa
     8521 ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata
     8581 tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt
     8641 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     8701 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     8761 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     8821 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
     8881 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
     8941 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
     9001 caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg
     9061 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg
     9121 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag
     9181 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg
     9241 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat
     9301 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac
     9361 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg
     9421 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt
     9481 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     9541 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     9601 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     9661 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     9721 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     9781 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     9841 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     9901 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     9961 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
    10021 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
    10081 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
    10141 accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg
    10201 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
    10261 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
    10321 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
    10381 tgacggtaca ggccagacaa ttat
//
