LOCUS       sequence-380580-         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Codon-optimized bacterial expression plasmid. Expresses 
            GB1-His6-TEV-A11(2-196, G189E)-Strep..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Shihora A, Elias RD, Hammond JA, Ghirlando R, Deshmukh L
  TITLE     ALS Variants of Annexin A11's Proline-Rich Domain Impair Its 
            S100A6-Mediated Fibril Dissolution.
  JOURNAL   ACS Chem Neurosci. 2023 Jul 11. doi: 10.1021/acschemneuro.3c00169.
  PUBMED    37433222
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Neurosci. 2023 Jul 11. doi: 10.1021/acschemneuro.3c00169."
COMMENT     Sequence Label: GB1-A11(2-196, G189E)-Strep
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             172..189
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             190..201
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             241..258
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             259..279
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             862..885
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 catatgcagt ataaactggc gctgaacggc aaaaccctga aaggcgaaac caccaccgaa
       61 gcggtggatg cggcgaccgc ggaaaaagtg tttaaacagt atgcgaacga taacggcgtg
      121 gatggcgaat ggacctatga tgatgcgacc aaaaccttta ccgtgaccga actggtgccg
      181 cgcggcagca ttgaaggccg cattcgcgaa aacctgaaag atagcggcct gtttctggaa
      241 catcatcatc atcatcatga aaacctgtat ttccaatctt atccgggcta tccgccgcca
      301 ccgggtgggt atccgccggc ggcaccgggc ggtggtccgt ggggcggtgc ggcgtatccg
      361 ccgcctccgt ctatgccgcc gattggcctg gataacgtgg cgacctatgc gggtcagttt
      421 aaccaagatt atctgagcgg catggcggcg aacatgagcg gcacctttgg cggtgcaaac
      481 atgccgaact tgtacccggg cgctccgggt gcggggtacc cgccggtacc acctggtggg
      541 tttgggcagc cgccgagcgc gcaacagcct gttccgccgt acgggatgta cccgccgccg
      601 ggcggcaacc caccgagccg catgccgtca tatccaccgt accctggggc accggtccct
      661 ggtcaaccga tgccaccgcc tgggcaacaa ccgccgggtg cttacccggg tcagccgcct
      721 gttacgtatc ctggtcagcc gccggtgccg ctcccgggtc aacagcaacc ggtgccatca
      781 tacccgggct acccgggcag cggcaccgta actccggcgg tgccgccgac gcagttcgag
      841 agcagaggca ccatcaccga ctggagccat ccgcagtttg aaaaataagg atcc
//
