LOCUS       sequence-380582-         462 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Codon-optimized bacterial expression plasmid. Expresses 
            GB1-His6-TEV-A11(2-52)-Strep..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Shihora A, Elias RD, Hammond JA, Ghirlando R, Deshmukh L
  TITLE     ALS Variants of Annexin A11's Proline-Rich Domain Impair Its 
            S100A6-Mediated Fibril Dissolution.
  JOURNAL   ACS Chem Neurosci. 2023 Jul 11. doi: 10.1021/acschemneuro.3c00169.
  PUBMED    37433222
REFERENCE   2  (bases 1 to 462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Neurosci. 2023 Jul 11. doi: 10.1021/acschemneuro.3c00169."
COMMENT     Sequence Label: GB1-A11(2-52)-Strep
FEATURES             Location/Qualifiers
     source          1..462
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             172..189
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             190..201
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             241..258
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             259..279
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             430..453
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 catatgcagt ataaactggc gctgaacggc aaaaccctga aaggcgaaac caccaccgaa
       61 gcggtggatg cggcgaccgc ggaaaaagtg tttaaacagt atgcgaacga taacggcgtg
      121 gatggcgaat ggacctatga tgatgcgacc aaaaccttta ccgtgaccga actggtgccg
      181 cgcggcagca ttgaaggccg cattcgcgaa aacctgaaag atagcggcct gtttctggaa
      241 catcatcatc atcatcatga aaacctgtat tttcaaagtt atccgggcta ccccccgcct
      301 cccggcggtt accctccggc cgcccctggc ggaggcccgt gggggggggc tgcgtaccct
      361 ccgcccccaa gcatgccgcc gattggcctg gataacgtgg cgacctatgc gggtcagttt
      421 aaccaagatt ggagccatcc gcagtttgaa aaataaggat cc
//
