LOCUS       sequence-380588-        8966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of tdTomato-tagged PEX3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8966)
  AUTHORS   Garbouchian A, Montgomery A, Gilbert SP, Bentley M
  TITLE     KAP is the neuronal organelle adaptor for Kinesin-2 KIF3AB and 
            KIF3AC.
  JOURNAL   Mol Biol Cell. 2022 Oct 6:mbcE22080336. doi: 
            10.1091/mbc.E22-08-0336.
  PUBMED    36200888
REFERENCE   2  (bases 1 to 8966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2022 Oct 6:mbcE22080336. doi: 10.1091/mbc.E22-08-0336."
COMMENT     Sequence Label: pBa.PEX3-tdTomato-FKBP
FEATURES             Location/Qualifiers
     source          1..8966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..276
                     /label=chicken beta-actin promoter
     primer_bind     1219..1241
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             2451..3878
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             3945..4265
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     polyA_signal    4385..4519
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4422..4441)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4476..4495
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4550..4566)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4550..4566)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4563..4585)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4574..4590
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4598..4604,4605..4622,4623..4628))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4643..4664
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4781..4798)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4952..5540)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5032..5051)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5711..6502,6503..6571))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6334..6353
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6572..6676)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6744..6762
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6800..6822)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6922..6941
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7135..7157
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7149..7166
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7150..7166
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
       61 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
      121 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
      181 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      241 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc
      301 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
      361 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
      421 taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc ttaaagggct ccgggagggc
      481 cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
      541 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
      601 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
      661 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
      721 tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc ccctccccga gttgctgagc
      781 acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg cgcggggctc gccgtgccgg
      841 gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg
      901 gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
      961 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     1021 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga
     1081 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc
     1141 gccgtcccct tctccatctc cagcctcggg gctgtccgca gggggacggc tgccttcggg
     1201 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggggtt tatatcttcc
     1261 cttctctgtt cctccgcagc ccccaagctt ggcgcgccac catgctgaga tcaatgtgga
     1321 attttctgaa acgtcacaaa aagaaatgca tcttcctggg cacagtcctc ggaggagtat
     1381 atatcctggg aaaatatgga cagaaaaaaa ttagagaaat acaagaaaga gaagctgcag
     1441 aatacattgc ccaagctcgg cgacagtacc attttgaaag caaccagagg acttgcaata
     1501 tgacagtgct gtccatgctg ccgacactga gagaggcctt aatgcagcag ctcgactcgg
     1561 agagtctcac agctctgttg aaaagcaggc cttcaaacaa gctggaaata tgggaggacc
     1621 taaagataat aagtttcaca agaagtattg tagctgtata tagcacgtgt atgctggtgg
     1681 ttcttttgcg agtccagtta aacataattg gtggatatat ttacctggat aatgcaacag
     1741 ttggcaaaaa tggcactacc gttcttgctc ctccggatgt acagcagcag tatttatcaa
     1801 gtattcaaca cctccttgga gacggcctca cagagttggt cactgtcatt aaacaagctg
     1861 tacaaaggat cttaggaagt gtctctctta aacattcctt gtcccttttg gacttggagc
     1921 aaaaactaaa agaaatcaga attcttgttg agcaacatca gtcttcttgg aatgataaag
     1981 atgtgtccag gtcttcactg tgccagtaca tgatgccaga tgaggagaca ccattggcag
     2041 ctcaggccta tgggctttct cacagagata tcaccactat taaacttctc aatgagacaa
     2101 gagacatgct ggaaagtcca gattttagta cagttttgaa tacctgtcta aacagaggct
     2161 tcagtcggct tctagacaac atggccgaat tctttcgacc cactgagcag gacctacagc
     2221 atggtaactc cataaacagt ctgtccagtg tcagcctgcc tttagctaag ataattccca
     2281 tagtgaacgg gcagatccat tcagtttgca gtgagacacc tagtcatttt gtgcaggatc
     2341 tgctgatgat ggaacaggtg aaagactttg ctgctaacgt gtacgaagcg tttagtaccc
     2401 cccaacaact ggagaaaggc ggctccggaa gcggaggccc taggcctacc atggtgagca
     2461 agggcgagga ggtcatcaaa gagttcatgc gcttcaaggt gcgcatggag ggctccatga
     2521 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     2581 ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc
     2641 cccagttcat gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc cccgattaca
     2701 agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     2761 gtctggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc tacaaggtga
     2821 agatgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct
     2881 gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccacc
     2941 aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagacc atctacatgg
     3001 ccaagaagcc cgtgcaactg cccggctact actacgtgga caccaagctg gacatcacct
     3061 cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc cgccaccacc
     3121 tgttcctggg gcatggcacc ggcagcaccg gcagcggcag ctccggcacc gcctcctccg
     3181 aggacaacaa catggccgtc atcaaagagt tcatgcgctt caaggtgcgc atggagggct
     3241 ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     3301 cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc
     3361 tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg
     3421 attacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     3481 acggcggtct ggtgaccgtg acccaggact cctccctgca ggacggcacg ctgatctaca
     3541 aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag aagaagacca
     3601 tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga
     3661 tccaccaggc cctgaagctg aaggacggcg gccgctacct ggtggagttc aagaccatct
     3721 acatggccaa gaagcccgtg caactgcccg gctactacta cgtggacacc aagctggaca
     3781 tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc gagggccgcc
     3841 accacctgtt cctgtacggc atggacgagc tgtacaagcg gaccggcgct agcggaggcg
     3901 gatctggagg cgggagcgga ggcggttccg gaggcgggac tagaggagtg caggtggaaa
     3961 ccatctcccc aggagacggg cgcaccttcc ccaagcgcgg ccagacctgc gtggtgcact
     4021 acaccgggat gcttgaagat ggaaagaaat ttgattcctc ccgggacaga aacaagccct
     4081 ttaagtttat gctaggcaag caggaggtga tccgaggctg ggaagaaggg gttgcccaga
     4141 tgagtgtggg tcagagagcc aaactgacta tatctccaga ttatgcctat ggtgccactg
     4201 ggcacccagg catcatccca ccacatgcca ctctcgtctt cgatgtggag cttctaaaac
     4261 tggaaactag ttattgagga tcataatcag ccatatcaca tctgtagagg ttttacttgc
     4321 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     4381 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     4441 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     4501 atcttatcat gtctggatcc ccgggtaccg agctcgaatt cgtaatcatg tcatagctgt
     4561 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     4621 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     4681 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     4741 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     4801 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     4861 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     4921 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     4981 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     5041 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     5101 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     5161 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     5221 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     5281 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     5341 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     5401 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     5461 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     5521 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     5581 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     5641 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     5701 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     5761 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     5821 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     5881 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     5941 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     6001 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     6061 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     6121 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     6181 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     6241 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     6301 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     6361 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     6421 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     6481 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     6541 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     6601 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     6661 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     6721 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     6781 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     6841 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     6901 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     6961 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
     7021 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     7081 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     7141 cacgacgttg taaaacgacg gccagtgcca agttgggatc tttgcattgg cccacggctc
     7201 tcaggatggg gatgctcccc ttcagcaccc ggttcccctt ggaaactgat ggtcctggct
     7261 ctgtggcatg gcagtggcac tgtgaggagc cccctaccag cagcacacag tggggttggc
     7321 actgccacgc tccggatgcc gcgctctgat ccaaccccat aatcaaggga acccgaattg
     7381 ccccatcatt gcccccacca cccccatcct gccgggccct cacaccccac gctgccttct
     7441 ggtgacattc cccagcccaa acccacggct tcatggctac cgcggggcat ttcccattgc
     7501 cgccccatta tcagctctgc acacctcccg ctgtacccat gcctcgtggc tgcccttctt
     7561 tgacgtataa tcttctaatt aatacccggc cttgtcaaag tggagcacaa acgttaatta
     7621 attccccagc aggcaggtaa ttaacagtgt gactcccttt ttgctgcgag tggggctgat
     7681 acagagagat gtggcactat ggagcccacg gggtcctggc actgggtgcc cacggaggtc
     7741 cccatgtgct gcagtgtcac cgcctccgag gagacagtat tgtccctgcg gtgtccctgc
     7801 agctcagctc tgtccacagg gccacctcca gtttggaggg gacacaatgc agccccgatg
     7861 caacccatcc tcgcagcatc ccagggacaa agaccccact gcaagaccgc acacagggct
     7921 gggtcccgct cccctaatat ctacagtgct tcttgcatgg ccccttaatc aatgcagtta
     7981 atcagcatgc gctcatgcac cgctctggag ctgcaaagcc cctcgcagcg ctgctcacca
     8041 acaccgcgca ccgccccggc ccagcctgca gcacgcgctg caaacaggaa agaaacaaaa
     8101 tattgcccaa atgtaggcaa aggcattcgg ctgccttgac ctccgccggg ccgggccctg
     8161 cctgactcag ctccttactc agcgctcgct tcctccctcc ggctgccacc gccgcagcgc
     8221 acaccctgac aaagagtggc ccttaacggg ctctgaggtg cacccagcag tgcactcagc
     8281 agtccaaggg ccggcctgga ggtttgcacc gctacgtgct gacattagca ttgaacttgg
     8341 ccctgggtag tgctgcaggc cgggcggggt gggtgtagag agtgcagcgc gcgttgcacc
     8401 cggtgcccct tcccctccct tgcatcccag caggctgcac cccagcacca ggcccgtgca
     8461 tgcatgctcc tggtgttatt gcagcctggt gcatgcatgc gtcttgatgg tgcagcgctg
     8521 tgcatgcatc ctccttggtg tgtagcagtc tagtgcatgc atacccctcg gtgttattgc
     8581 tgctctgtgc acgcacgctc attgtatcac ttcatcccag tgcatgcact cacactggag
     8641 cgattgctgc tcggtgcacg cacactcatt gtatcactgc agctcagtgg ctgcacgcac
     8701 accggtgtta ttgctgctcg gtgcgtgcat gcacatcagt gtcgctgcag ctcagtgcat
     8761 gcacgctcat tgcccatcgc tatccctgcc tctcctgctg gcgctccccg ggaggtgact
     8821 tcaaggggac cgcaggacca cctcgggggt ggggggaggg ctgcacacgc ggaccccgct
     8881 ccccctcccc aacaaagcac tgtggaatca aaaagggggg aggggggatg gaggggcgcg
     8941 tcacaccccc gccccacacc ctcacc
//
