LOCUS       sequence-380603-        9281 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Heterologous expression of Tse5 in E. coli Lemo21 cells for 
            purification of Tse5-CT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Gonzalez-Magana A, Altuna J, Queralt-Martin M, Largo E, Velazquez C,
            Montanchez I, Bernal P, Alcaraz A, Albesa-Jove D
  TITLE     The P. aeruginosa effector Tse5 forms membrane pores disrupting the 
            membrane potential of intoxicated bacteria.
  JOURNAL   Commun Biol. 2022 Nov 5;5(1):1189. doi: 10.1038/s42003-022-04140-y.
  PUBMED    36335275
REFERENCE   2  (bases 1 to 9281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-11-5"; volume: "5(1):1189. doi"; pages: " 
            10.1038/s42003-022-04140-y"
COMMENT     Sequence Label: pET29a(+)::9xhis-Tse5
FEATURES             Location/Qualifiers
     source          1..9281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(99..118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     309..330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(559..1374)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(559..1374)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1282..1301
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1366..1385
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1496..2084
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1985..2004
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2238..2255
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2270..2409
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2335..2354)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2495..2517
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2511..2702)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3511..4593)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4555..4574
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4594..4671)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     4877..4896
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4984..5003
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4984..5002
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5003..5027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5042..5064
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5087..5113
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     CDS             5123..5143
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             9125..9142
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9195..9213)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      9209..9256
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
      481 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
      541 ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
      601 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
      661 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
      721 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
      781 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca
      841 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
      901 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca
      961 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
     1021 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt
     1081 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
     1141 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
     1201 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt
     1261 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
     1321 gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc
     1381 ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac
     1441 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     1501 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     1561 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     1621 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     1681 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     1741 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     1801 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     1861 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     1921 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     1981 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2041 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2101 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     2161 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     2221 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     2281 attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc tcagtacaat
     2341 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     2401 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     2461 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     2521 tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga
     2581 agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc
     2641 gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc
     2701 actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga
     2761 gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt
     2821 gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa
     2881 tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg
     2941 atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa
     3001 acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag
     3061 tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc
     3121 cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ggccgccatg
     3181 ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct
     3241 tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc
     3301 cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt
     3361 tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg
     3421 aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga
     3481 gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     3541 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     3601 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc
     3661 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc
     3721 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc
     3781 cactaccgag atgtccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc
     3841 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat
     3901 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg
     3961 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac
     4021 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc
     4081 cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc
     4141 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc
     4201 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg
     4261 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc
     4321 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc
     4381 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac
     4441 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc
     4501 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata
     4561 ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc
     4621 cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc
     4681 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt
     4741 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg
     4801 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag
     4861 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc
     4921 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatcgatctc gatcccgcga
     4981 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
     5041 ttttgtttaa ctttaagaag gagatataca tatgatgggc agcagccatc atcatcatca
     5101 tcatcatcat cacagcagcg gcgaaaacct gtattttcag ggcggatcca tgagcggcct
     5161 gccggttagc catgtgggcg aaaaagttag cggcggcgtt attagcaccg gtagcccgac
     5221 cgttcacgtt ggtagcagcg cggtgggcct ggcggaccgc gtgagcgcct gcgtgccgct
     5281 ggtgggcaag ccggtgaacc cgatgctggg cagcaaactg ctgccggagg aagtggactt
     5341 cgccctggcc gccccggaca ccttcacctt cgcccgtggt tacctgagca gcaacccgcg
     5401 tatcggtcgt ctgggtcgtg gttggtggct gccgggcgag agcatgcacc tggaactgag
     5461 cgaggacgcc tgcgtgctgg tggacgccca gggtcgccgt atcggtttcc cggcgctggc
     5521 cccgggtgcc cagcactaca gcggcagcga ggaactgtgg ctgcgccgtg gcggtagcag
     5581 cggcggtgaa gcgcaggcct ggcgcggtcg ttgggcggcc gtgccggccg agctgcagac
     5641 ccaggaaggt agcgtgctgg tgctgagcgg ccacagctac ctgcacttcc agcgttgccc
     5701 ggacggcatc tggcgcctgc aggcgagctt cggtcgtgcg ggttaccgca ccgagttccg
     5761 ctggagcggt cgtggtctgc tgaccggtgt gcgtgacagc gccggtcgca gctacgccct
     5821 ggtgtaccag caggcctgcg aaccgagcga gggtgacgac ggtctgcgtc tgttcggtgt
     5881 gatcctggcg agccacgacg gtccgccgcc ggactacatc gacccgcaga gcccgggcct
     5941 ggactggctg gtgcgctacc agttcagcga cagcggtgac ctgatcgcgg tgcgtgaccg
     6001 cctgggtcag gtggtgcgtg tgttcgcctg gcgcgaacac atgctggtgg cccacggcga
     6061 gccgggcggt ctggaagtgc gttacgagtg ggacgtgcac gccccgcacg gtcgtgtggt
     6121 gaagcagatc gaagcgggcg gtctgacccg taccttccgt tacctgcgcg acgccaccga
     6181 ggtgagcgac agcctgggtc gtgtggaacg ctacgagttc gccggtgaag gcggtcagcg
     6241 ccgttggacc gccctggtgc gtgccgatgg cagccgcagc gaattcgact acgacctgtt
     6301 cggtcgtctg gtggccatgc gcgacccgct gggtcgtgaa acccgccgtc gccgtgacgg
     6361 tcagggtcgt atgctggagg aagagagccc gggcaaggcg cgttaccgca aacgtgtgga
     6421 cgaagaaacc ggcctgctgg tggaactgga ggacgccatg cagcgccgtt ggaccttcga
     6481 acgtgacgaa cgtggtaacg ccaccaccgt tcgtggtccg gcgggtagca cccgctacgc
     6541 ctacgaggac ccgcgtctgc cggaccgtcc gacccgtatc gtggacccgc gtggcggtga
     6601 acgccgtctg gagtggaacc gtttcggtct gctggccgcc ctgaccgact gcagcggtca
     6661 agtgtggcgt tacgactacg acaacgaagg tcgtctggtg gccagcagcg acccgctggg
     6721 tcagctgacc cgccgtcgct acgacccgct gggccagctg atcggtctgg aactggccga
     6781 cggcagcgcc ctgagctacg agtacgacgc cctgggtcgt cagacccgta tcgccgacgc
     6841 ggagggtcac gccaccctgt tcagctgggg tcacggtgac ctgctggcgc gtgtgagcga
     6901 cgccggcggt ggcgagctga gctacctgca cgacgaagcc ggccgtctgg tggccctgac
     6961 caacgaaaac ggcgtgcagg cccagttccg ctacgacctg ctggaccgtc tggtggaaga
     7021 aaccggtttc gacggtcgtc gccagcgtta ccgttacaac gcggccgacg aactgatcgc
     7081 ccgtgaagac gccgacggtc gtgaaaccac ctacgcctac gaccgtgacg gtcgcctggc
     7141 gagcatccgt gtgccggcca ccgagcacgc cccggccctg gtggaacgtt accgttggct
     7201 ggccgatggc cgcctggcga gcgccggtgg cgcggactgc gaagtgcgtt acacctacga
     7261 cgaagtgggt aacctgcgtc tggagagcca ggtgcacgcg gacggctggg tgtacagcgt
     7321 ggaacacagc cacgacgccc tgggtgtgcg tcagaccagc cgttacggtg acgcaccgcc
     7381 ggtggcgtgg ctgacctacg gcccgggtca cctgcacggt gccctggtgg gtgccgtgga
     7441 actggccttc gagcgtgacg ccctgcaccg tgaagtgcgt cgcgacgccc gtcgcgacgg
     7501 tcaggacgac gcgctgttca cccaggaacg tcagcacgcc ccgctgggtc gtctgcagcg
     7561 cagccgtctg cgcctggcgg gtggcttcga ctggcagcgt ggttaccgtt acgacggcct
     7621 gggtcagctg gtgggcatcg acgacaacca gtacccgagc gtgcgttacg agtacgacct
     7681 gggtggccgt ctgctggcga gccgtcgcgc gggtgcggcc gccagcacct accgctacga
     7741 cgccgcgggt aaccgcctgg agggtgtggg tgaacacgcc cgtgaagacg cccgtcaggc
     7801 gttcgccgaa aacgagctgt accgtagcgg cttcagccgt agcgaaaccc gcgccagcca
     7861 ggccggtgaa ggtccggccc gttgggccgg taaccgtgtg gagcgtatcg ccggcaaccg
     7921 ttaccgcttc gacgcgctgg gcaacctggt ggaacgtatc ggtgcggacg gcgagcgtct
     7981 gcgcctggcg tacgacggtg cgcagcgtct ggtgcacctg acccgcgact acgccgacgg
     8041 tacccgtctg gaagcccgtt accgctacga cgccctgagc cgtcgcatcg ccaaggtggt
     8101 gctgcgtgac ggtgtggaac agcaagtgcg tttcggttgg gacggtgacc gccagtgcgc
     8161 cgaggccttc gcccgtgaac tgcgtaccac cgtgcacgag ccgggtggct tcgtgccgct
     8221 gctgcgtctg gagcaggcct gcgaaccgga cccgccggaa ctgctgcagc tgcgtcaggc
     8281 gttcgccgcg gagggtcagc cgctgccggc ccagtgcgtg ccggccctgg gcgaagcccg
     8341 catcgcgttc ttccacaccg accacctggg taccccgctg cagctgagcg acgagcgtgg
     8401 tcagctgcgc tggcagggtg tgccggacga ctggcgtgcc gtggcgccgg aacgtcagcc
     8461 gggtgcccag ccgatccgtt tccagggcca gtaccacgac gaagagagcg gtctgtacta
     8521 caaccgttac cgctactacc tgccggaggc cggtcgttac gccagccagg acccgctggg
     8581 tctgggtggc ggtccgaacc cgtacgccta cgcgctgaac gccccgaccc tggcctacga
     8641 cccgaccggc ctgatcatcc cgctggtggt gatcggtgcc ttcgccgccc gtgccgccat
     8701 cggtgccgcc ctgggcgcgg gtatcgaact gggcatgcag accggcaagc aggtgctggg
     8761 ccagatgaaa gacaactggg acagcgaccg cgacctgacc gacatcaagt ggaaatgcat
     8821 cgacatcaac tggaaacacg tgggtgccag cgccgccatc ggtaccgtgg ccccgggtat
     8881 gctgagcacc ggcaagaccg tggtgcagag cgccaaagcc atccgtaccc tgagcggtca
     8941 ggccgccaac accgccaacc gtgccgccaa gctggcggcc cgtaaagccg cccacgcgga
     9001 caccatcaag aaagccgtgg cgacccaggc ggcctggcag accggtaaac agattgtgaa
     9061 gtgcccgctg aaagacgagg aagaagaatg cccgccgcag taaaagcttg cggccgcact
     9121 cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga
     9181 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
     9241 cttgaggggt tttttgctga aaggaggaac tatatccgga t
//
