LOCUS       sequence-38062-e         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  TITLE     Linda Hicke lab unpublished Ubiquitin constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: LHP398 - Ub-K48R/K63R
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..230
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGRQLEDGRTLSDYNIQRESTLHLVLRLRGG"
     terminator      343..590
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(359..377)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        623..724
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
ORIGIN
        1 ttatgcagat cttcgtcaag acgttaaccg gtaaaaccat aactctagaa gttgaatctt
       61 ccgataccat cgacaacgtt aagtcgaaaa ttcaagacaa ggaaggcatt ccacctgatc
      121 aacaaagatt gatctttgcc ggtagacagc tcgaggacgg tagaacgctg tctgattaca
      181 acattcagag ggagtcgacc ttacatcttg tcttaagact aagaggtggt tgaggtacca
      241 agatggcctt tggtgggttg aagaaggaaa aagacagaaa cgacttaatt acctacttga
      301 aaaaagcctg tgagtaaaca ggcccctttt cctttgtcga tatcatgtaa ttagttatgt
      361 cacgcttaca ttcacgccct ccccccacat ccgctctaac cgaaaaggaa ggagttagac
      421 aacctgaagt ctaggtccct atttattttt ttatagttat gttagtatta agaacgttat
      481 ttatatttca aatttttctt ttttttctgt acagacgcgt gtacgcatgt aacattatac
      541 tgaaaacctt gcttgagaag gttttgggac gctcgaaggc tttaatttgc aagcttatcg
      601 atgataagct gtcaaacatg agaattcggt cgaaaaaaga aaaggagagg gccaagaggg
      661 agggcattgg tgactattga gcacgtgagt atacgtgatt aagcacacaa aggcagcttg
      721 gagtatgtct gttattaatt tcacagg
//
