LOCUS       sequence-38063-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  TITLE     Linda Hicke lab unpublished Ubiquitin constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: LHP809 - Ub-K63A-CYC1
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      325..572
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(341..359)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(591..607)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(591..607)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(604..626)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    615..631
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(639..645,646..663,664..669))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    684..705
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(822..839)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agacgttaac cggtaaaacc ataactctag aagttgaatc ttccgatacc atcgacaacg
       61 ttaagtcgaa aattcaagac aaggaaggca ttccacctga tcaacaaaga ttgatctttg
      121 ccggtaagca gctcgaggac ggtagaacgc tgtctgatta caacattcag gcggagtcga
      181 ccttacatct tgtcttaaga ctaagaggtg gttgaggtac caagatggcc tttggtgggt
      241 tgaagaagga aaaagacaga aacgacttaa ttacctactt gaaaaaagcc tgtgagtaaa
      301 caggcccctt ttcctttgtc gatatcatgt aattagttat gtcacgctta cattcacgcc
      361 ctccccccac atccgctcta accgaaaagg aaggagttag acaacctgaa gtctaggtcc
      421 ctatttattt ttttatagtt atgttagtat taagaacgtt atttatattt caaatttttc
      481 ttttttttct gtacagacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga
      541 aggttttggg acgctcgaag gctttaattt gcaagcttgg cgtaatcatg gtcatagctg
      601 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
      661 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
      721 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
      781 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
      841 cgctcggtcg ttcg
//
