LOCUS       sequence-38064-e         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  TITLE     Linda Hicke lab unpublished Ubiquitin constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: LHP327 - Ub-F45A/G76A
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             8..232
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIA
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRG"
     terminator      348..595
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(364..382)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 attcattatg cagatcttcg tcaagacgtt aaccggtaaa accataactc tagaagttga
       61 atcttccgat accatcgaca acgttaagtc gaaaattcaa gacaaggaag gcattccacc
      121 tgatcaacaa agattgatcg ctgccggtaa gcagctcgag gacggtagaa cgctgtctga
      181 ttacaacatt cagaaggagt cgaccttaca tcttgtctta agactaagag gtgcttgagg
      241 taccaagatg gcctttggtg ggttgaagaa ggaaaaagac agaaacgact taattaccta
      301 cttgaaaaaa gcctgtgagt aaacaggccc cttttccttt gtcgatatca tgtaattagt
      361 tatgtcacgc ttacattcac gccctccccc cacatccgct ctaaccgaaa aggaaggagt
      421 tagacaacct gaagtctagg tccctattta tttttttata gttatgttag tattaagaac
      481 gttatttata tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg catgtaacat
      541 tatactgaaa accttgcttg agaaggtttt gggacgctcg aaggctttaa tttgcaagct
      601 tatcgatgat aagctgtcaa acatgagaat tcggtcgaaa aaagaaaagg agagggccaa
      661 gagggagggc attggtgact attgagcacg tgagtatacg tgattaagca cacaaaggca
      721 gcttg
//
