LOCUS       sequence-38067-e         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Sander JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung JK, Yeh JR
  TITLE     Targeted gene disruption in somatic zebrafish cells using engineered
            TALENs.
  JOURNAL   Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934.
  PUBMED    21822241
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2011-08-5- 5"; volume: "29"; 
            issue: "8"; pages: "697-8"
COMMENT     Sequence Label: TALEN1257
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             54..119
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             126..146
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atcgaaatta atacgactca cctataggga gacccaagct ggctagcacc atggactaca
       61 aagaccatga cggtgattat aaagatcatg acatcgatta caaggatgac gatgacaaga
      121 tggcccccaa gaagaagagg aaggtgggca ttcaccgcgg ggtacctatg gtggacttga
      181 ggacactcgg ttattcgcaa cagcaacagg agaaaatcaa gcctaaggtc aggagcaccg
      241 tcgcgcaaca ccacgaggcg cttgtggggc atggcttcac tcatgcgcat attgtcgcgc
      301 tttcacagca ccctgcggcg cttgggacgg tggctgtcaa ataccaagat atgattgcgg
      361 ccctgcccga agccacgcac gaggcaattg taggggtcgg taaacagtgg tcgggagcgc
      421 gagcacttga ggcgctgctg actgtggcgg gtgagcttag ggggcctccg ctccagctcg
      481 acaccgggca gctgctgaag atcgcgaaga gagggggagt aacagcggta gaggcagtgc
      541 acgcctggcg caatgcgctc accggggccc ccttgaacct gaccccagac caggtagtcg
      601 caatcgcgtc aaacggaggg ggaaagcaag ccctggaaac cgtgcaaagg ttgttgccgg
      661 tcctttgtca agaccacggc cttacaccgg agcaagtcgt ggccattgca agcaatgggg
      721 gtggcaaaca ggctcttgag acggttcaga gacttctccc agttctctgt caagcccacg
      781 ggctgactcc cgatcaa
//
