LOCUS       sequence-38068-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Sander JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung JK, Yeh JR
  TITLE     Targeted gene disruption in somatic zebrafish cells using engineered
            TALENs.
  JOURNAL   Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934.
  PUBMED    21822241
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2011-08-5- 5"; volume: "29"; 
            issue: "8"; pages: "697-8"
COMMENT     Sequence Label: TALEN1258
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..33
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14..32
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             58..123
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             130..150
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggcttatcga aattaatacg actcactata gggagaccca agctggctag caccatggac
       61 tacaaagacc atgacggtga ttataaagat catgacatcg attacaagga tgacgatgac
      121 aagatggccc ccaagaagaa gaggaaggtg ggcattcacc gcggggtacc tatggtggac
      181 ttgaggacac tcggttattc gcaacagcaa caggagaaaa tcaagcctaa ggtcaggagc
      241 accgtcgcgc aacaccacga ggcgcttgtg gggcatggct tcactcatgc gcatattgtc
      301 gcgctttcac agcaccctgc ggcgcttggg acggtggctg tcaaatacca agatatgatt
      361 gcggccctgc ccgaagccac gcacgaggca attgtagggg tcggtaaaca gtggtcggga
      421 gcgcgagcac ttgaggcgct gctgactgtg gcgggtgagc ttagggggcc tccgctccag
      481 ctcgacaccg ggcagctgct gaagatcgcg aagagagggg gagtaacagc ggtagaggca
      541 gtgcacgcct ggcgcaatgc gctcaccggg gcccccttga acctgacccc agaccaggta
      601 gtcgcaatcg cgtcacatga cgggggaaag caagccctgg aaaccgtgca aaggttgttg
      661 ccggtccttt gtcaagacca cggccttaca ccggagcaag tcgtggccat tgcaaataat
      721 aacggtggca aacaggctct tgagacggtt cagagacttc tcccagttct ctgtcaagcc
      781 cacgggctga ctcccgatca agttgtagcg attgcgtcca acggtggagg
//
