LOCUS       sequence-38069-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Sander JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung JK, Yeh JR
  TITLE     Targeted gene disruption in somatic zebrafish cells using engineered
            TALENs.
  JOURNAL   Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934.
  PUBMED    21822241
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2011-08-5- 5"; volume: "29"; 
            issue: "8"; pages: "697-8"
COMMENT     Sequence Label: TALEN1260
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             72..137
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             144..164
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cccactggct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg
       61 ctagcaccat ggactacaaa gaccatgacg gtgattataa agatcatgac atcgattaca
      121 aggatgacga tgacaagatg gcccccaaga agaagaggaa ggtgggcatt caccgcgggg
      181 tacctatggt ggacttgagg acactcggtt attcgcaaca gcaacaggag aaaatcaagc
      241 ctaaggtcag gagcaccgtc gcgcaacacc acgaggcgct tgtggggcat ggcttcactc
      301 atgcgcatat tgtcgcgctt tcacagcacc ctgcggcgct tgggacggtg gctgtcaaat
      361 accaagatat gattgcggcc ctgcccgaag ccacgcacga ggcaattgta ggggtcggta
      421 aacagtggtc gggagcgcga gcacttgagg cgctgctgac tgtggcgggt gagcttaggg
      481 ggcctccgct ccagctcgac accgggcagc tgctgaagat cgcgaagaga gggggagtaa
      541 cagcggtaga ggcagtgcac gcctggcgca atgcgctcac cggggccccc ttgaacctga
      601 ccccagacca ggtagtcgca atcgcgtcga acattggggg aaagcaagcc ctggaaaccg
      661 tgcaaaggtt gttgccggtc ctttgtcaag accacggcct tacaccggag caagtcgtgg
      721 ccattgcaag caacatcggt ggcaaacagg ctcttgagac ggttcagaga cttctcccag
      781 ttctctgtca agcccacggg ctgactcccg atcaagttgt agcgattgcg tcgaacattg
//
