LOCUS       sequence-380701-        9045 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SGLT1 protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9045)
  TITLE     Jun Liu Lab Plasmids 2022
REFERENCE   2  (bases 1 to 9045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti6M SGLT1
FEATURES             Location/Qualifiers
     source          1..9045
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      591..600
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             600..623
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             642..662
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             666..707
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2740..2781
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(2818..2838)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2823..3152
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3003..3138
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        3200..3247
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3266..3664
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             3751..3984
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4056..4177
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4093..4112)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4147..4166
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4217..4352
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4372..4391)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4373..4391)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4401..4418)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4401..4417)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4410..4432)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4559..5014
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4646..4665)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4856..4877
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5040..5144
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5145..5213,5214..6005)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5363..5382)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6176..6764
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6665..6684
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6918..6935
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7052..7073
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7088..7093,7094..7111,7112..7118)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7126..7142
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7131..7153
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7150..7166
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7150..7166
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7185..7205
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7187..7205
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7233..7459
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7460..7640
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7687..7812
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8305..8538
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8723..8767
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgactc tagaggatcc gccaccatgg
      601 attacaagga tgacgacgat aagagcccgg gcggatcgtt ggagaatctt tattttcagg
      661 gtcaaggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtct agactgcaga
      721 tggacagtag cacctggagc cccaagacca ccgcggtcac ccggcctgtt gagacccacg
      781 agctcattcg caatgcagcc gatatctcca tcatcgttat ctacttcgtg gtagtgatgg
      841 ccgtcggact gtgggctatg ttttccacca atcgtgggac tgttggaggc ttcttcctgg
      901 caggccgaag tatggtgtgg tggccgattg gagcctccct ctttgctagt aacattggaa
      961 gtggccactt tgtggggctg gccgggactg gggcagcttc aggcatcgcc attggaggct
     1021 ttgaatggaa tgccctggtt ttggtggttg tgctgggctg gctgtttgtc cccatctata
     1081 ttaaggctgg ggtggtgaca atgccagagt acctgaggaa gcggtttgga ggccagcgga
     1141 tccaggtcta cctttccctt ctgtccctgc tgctctacat tttcaccaag atctcggcag
     1201 acatcttctc gggggccata ttcatcaatc tggccttagg cctgaatctg tatttagcca
     1261 tctttctctt attggcaatc actgcccttt acacaattac agggggcctg gcggcggtga
     1321 tttacacgga caccttgcag acggtgatca tgctggtggg gtctttaatc ctgactgggt
     1381 ttgcttttca cgaagtggga ggctatgacg ccttcatgga aaagtacatg aaagccattc
     1441 caaccatagt gtctgatggc aacaccacct ttcaggaaaa atgctacact ccaagggccg
     1501 actccttcca catcttccga gatcccctca cgggagacct cccatggcct gggttcatct
     1561 ttgggatgtc catccttacc ttgtggtact ggtgcacaga tcaggtcatt gtgcagcgct
     1621 gcctctcagc caagaatatg tctcacgtga agggtggctg catcctgtgt gggtatctaa
     1681 agctgatgcc catgttcatc atggtgatgc caggaatgat cagccgcatt ctgtacacag
     1741 aaaaaattgc ctgtgtcgtc ccttcagaat gtgagaaata ttgcggtacc aaggttggct
     1801 gtaccaacat cgcctatcca accttagtgg tggagctcat gcccaatgga ctgcgaggcc
     1861 tgatgctatc agtcatgctg gcctccctca tgagctccct gacctccatc ttcaacagcg
     1921 ccagcaccct cttcaccatg gacatctacg ccaaggtccg caagagagca tctgagaaag
     1981 agctcatgat tgccggaagg ttgtttatcc tggtgctgat tggcatcagc atcgcctggg
     2041 tgcccattgt gcagtcagca caaagtgggc aactcttcga ttacatccag tccatcacca
     2101 gttacttggg accacccatt gcggctgtct tcctgcttgc tattttctgg aagagagtca
     2161 atgagccagg agccttttgg ggactgatcc taggacttct gattgggatt tcacgtatga
     2221 ttactgagtt tgcttatgga accgggagct gcatggagcc cagcaactgt cccacgatta
     2281 tctgtggggt gcactacttg tactttgcca ttatcctctt cgccatttct ttcatcacca
     2341 tcgtggtcat ctccctcctc accaaaccca ttccggatgt gcatctctac cgtctgtgtt
     2401 ggagcctgcg caacagcaaa gaggagcgta ttgacctgga tgcggaagag gagaacatcc
     2461 aagaaggccc taaggagacc attgaaatag aaacacaagt tcctgagaag aaaaaaggaa
     2521 tcttcaggag agcctatgac ctattttgtg ggctagagca gcacggtgca cccaagatga
     2581 ctgaggaaga ggagaaagcc atgaagatga agatgacgga cacctctgag aagcctttgt
     2641 ggaggacagt gttgaacgtc aatggcatca tcctggtgac cgtggctgtc ttttgccatg
     2701 catattttgc ctgactcgag ggggggcccg cggttcgaag gtaagcctat ccctaaccct
     2761 ctcctcggtc tcgattctac gcgtaccggt tagtaatgag tttggaatta attctgtgga
     2821 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     2881 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     2941 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     3001 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     3061 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     3121 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     3181 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
     3241 atacgacaag gtgaggaact aaaccatggc caagcctttg tctcaagaag aatccaccct
     3301 cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc
     3361 cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac
     3421 tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa
     3481 cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg
     3541 gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga
     3601 tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg
     3661 ctaagcacaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     3721 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     3781 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     3841 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     3901 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     3961 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     4021 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     4081 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     4141 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     4201 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     4261 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     4321 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     4381 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     4441 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     4501 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     4561 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4621 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4681 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4741 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     4801 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4861 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     4921 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4981 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     5041 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5101 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     5161 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5221 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     5281 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     5341 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     5401 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5461 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     5521 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     5581 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     5641 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     5701 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     5761 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     5821 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     5881 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     5941 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     6001 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     6061 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     6121 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6181 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6241 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6301 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     6361 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6421 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6481 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6541 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6601 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6661 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6721 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6781 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     6841 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     6901 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     6961 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     7021 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     7081 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     7141 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     7201 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
     7261 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
     7321 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
     7381 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
     7441 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
     7501 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7561 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7621 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     7681 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     7741 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     7801 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     7861 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     7921 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     7981 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     8041 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     8101 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     8161 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     8221 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     8281 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     8341 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     8401 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     8461 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     8521 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     8581 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     8641 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     8701 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     8761 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     8821 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     8881 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     8941 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     9001 attagtgaac ggatctcgac ggtatcgata agcttgggag ttccg
//
