LOCUS       sequence-380704-        9312 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation, Inducible lentiviral expression, TRE-gateway; 
            PGK-rtTA-2A-blasti.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9312)
  TITLE     Superti-Furga RESOLUTE modified pLIX plasmids
REFERENCE   2  (bases 1 to 9312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCW57.1_TStrp_HA_Nt
FEATURES             Location/Qualifiers
     source          1..9312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    27..144
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        198..512
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    206..224
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    242..260
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    277..295
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    313..331
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    349..367
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    384..402
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    420..438
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     485..509
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             539..565
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             596..619
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             656..679
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    692..816
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(841..846,847..864,865..871)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             925..1584
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(991..1010)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1926..2231
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2110..2129
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2272..2396)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2414..2431
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        2441..2951
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2870..2888
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2961..3356
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             3357..3410
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3411..4028,4029..4034,4035..4070,4071..4073,4074..4109,
                     4110..4112,4113..4148,4149..4157)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4197..4217)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4615..4626)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4831..5064
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5136..5257
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5173..5192)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5227..5246
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5297..5432
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5432..5503
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5504..5572,5573..6364)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5722..5741)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6535..7123
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7024..7043
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7277..7294
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7411..7432
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7447..7452,7453..7470,7471..7477)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7485..7501
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7490..7512
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7509..7525
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7509..7525
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7544..7564
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7546..7564
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7592..7818
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7819..7999
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8046..8171
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8664..8897
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9082..9126
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
       61 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
      121 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg
      181 ccgcgaatcg atatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta
      241 ctccctatca gtgatagaga acgatgtcga gtttactccc tatcagtgat agagaacgta
      301 tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
      361 atagagaacg tatgtcgagt ttatccctat cagtgataga gaacgtatgt cgagtttact
      421 ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt acggtgggag gcctatataa
      481 gcagagctcg tttagtgaac cgtcagatcg cctggagaat tggctagcat caaccatgta
      541 cccatacgat gttcctgact atgccggtac cgagctcgga tccaccatgg ctagctggag
      601 ccacccgcag ttcgagaaag gtggaggttc cggaggtgga tcgggaggtg gatcgtggag
      661 ccacccgcag ttcgaaaaag cggccgatat cacaagtttg tacaaaaaag ctgaacgaga
      721 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
      781 ataatactgt aaaacacaac atatccagtc actatggcgg ccgcattagg caccccaggc
      841 tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga
      901 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
      961 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
     1021 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
     1081 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
     1141 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
     1201 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
     1261 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
     1321 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt
     1381 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
     1441 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
     1501 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc
     1561 gatgagtggc agggcggggc gtaaagatct ggatccggct tactaaaagc cagataacag
     1621 tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga
     1681 agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca
     1741 gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat
     1801 gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat
     1861 ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg
     1921 gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg
     1981 atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg
     2041 cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg
     2101 aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag
     2161 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct
     2221 ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac
     2281 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
     2341 atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggtttag
     2401 taatgaaccg gtccaccacc accaccacca ctaaggatcc ggggttgggg ttgcgccttt
     2461 tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt tccgggaaac
     2521 gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga
     2581 tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg
     2641 aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta
     2701 gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg cgatgggctg
     2761 tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc
     2821 gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat
     2881 tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga atcaccgacc
     2941 tctctcccca gcaattcacc atggccaagc ctttgtctca agaagaatcc accctcattg
     3001 aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg
     3061 cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg
     3121 gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga
     3181 cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc
     3241 gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac
     3301 agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggcgaag
     3361 gtagaggttc tctcctcact tgtggtgatg ttgaagaaaa ccctggtcca atgtctagac
     3421 tggacaagag caaagtcata aacggagctc tggaattact caatggtgtc ggtatcgaag
     3481 gcctgacgac aaggaaactc gctcaaaagc tgggagttga gcagcctacc ctgtactggc
     3541 acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat cgagatgctg gacaggcatc
     3601 atacccactt ctgccccctg gaaggcgagt catggcaaga ctttctgcgg aacaacgcca
     3661 agtcataccg ctgtgctctc ctctcacatc gcgacggggc taaagtgcat ctcggcaccc
     3721 gcccaacaga gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg tgtcagcaag
     3781 gcttctccct ggagaacgca ctgtacgctc tgtccgccgt gggccacttt acactgggct
     3841 gcgtattgga ggaacaggag catcaagtag caaaagagga aagagagaca cctaccaccg
     3901 attctatgcc cccacttctg agacaagcaa ttgagctgtt cgaccggcag ggagccgaac
     3961 ctgccttcct tttcggcctg gaactaatca tatgtggcct ggagaaacag ctaaagtgcg
     4021 aaagcggcgg gccgaccgac gcccttgacg attttgactt agacatgctc ccagccgatg
     4081 cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat tttgaccttg
     4141 acatgctccc cgggtaacta agtaaggatc gatccaagat atcgtattct taactatgtt
     4201 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     4261 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     4321 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     4381 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     4441 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     4501 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     4561 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     4621 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     4681 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgtttcg
     4741 cctcggcgtc cggactagag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     4801 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     4861 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     4921 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     4981 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     5041 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     5101 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     5161 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     5221 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     5281 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     5341 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     5401 ccagaagtag tgaggaggct tttttggagg cctttcaaat atgtatccgc tcatgagaca
     5461 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     5521 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     5581 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     5641 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     5701 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     5761 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     5821 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     5881 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     5941 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     6001 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     6061 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     6121 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     6181 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     6241 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     6301 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     6361 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     6421 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     6481 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     6541 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6601 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6661 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     6721 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6781 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6841 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6901 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6961 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     7021 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     7081 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     7141 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     7201 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     7261 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     7321 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     7381 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     7441 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     7501 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
     7561 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
     7621 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
     7681 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
     7741 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
     7801 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     7861 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     7921 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     7981 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     8041 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     8101 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     8161 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     8221 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     8281 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     8341 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     8401 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     8461 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     8521 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     8581 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     8641 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     8701 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     8761 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     8821 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     8881 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     8941 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     9001 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     9061 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     9121 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     9181 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     9241 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggacaa
     9301 ttctcgacct cg
//
