LOCUS       sequence-380706-        9153 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation, Inducible lentiviral expression, TRE-gateway; 
            PGK-rtTA-2A-blasti.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9153)
  TITLE     Superti-Furga RESOLUTE modified pLIX plasmids
REFERENCE   2  (bases 1 to 9153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCW57.1_blasti
FEATURES             Location/Qualifiers
     source          1..9153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    47..164
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        218..532
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    226..244
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    262..280
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    297..315
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    333..351
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    369..387
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    404..422
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    440..458
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    553..677
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(702..707,708..725,726..732)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             786..1445
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(852..871)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1787..2092
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1971..1990
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2133..2257)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2275..2292
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        2302..2812
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2731..2749
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2822..3217
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             3218..3271
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3272..3889,3890..3895,3896..3931,3932..3934,3935..3970,
                     3971..3973,3974..4009,4010..4018)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4058..4078)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4476..4487)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4692..4925
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4997..5118
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5034..5053)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5088..5107
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5158..5293
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5293..5364
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5365..5433,5434..6225)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5583..5602)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6396..6984
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6885..6904
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7138..7155
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7272..7293
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7308..7313,7314..7331,7332..7338)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7346..7362
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7351..7373
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7370..7386
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7370..7386
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7405..7425
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7407..7425
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7453..7679
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7680..7860
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7907..8032
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8525..8758
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8943..8987
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ggggacaatt ctcgacctcg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
       61 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
      121 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
      181 acagcagaga tccactttgg ccgcgaatcg atatgtcgag tttactccct atcagtgata
      241 gagaacgtat gtcgagttta ctccctatca gtgatagaga acgatgtcga gtttactccc
      301 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
      361 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttatccctat cagtgataga
      421 gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt
      481 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagaat
      541 tggctagcat caacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat
      601 atcaatatat taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa
      661 catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt tatgcttccg
      721 gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga gctaaggaag
      781 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
      841 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
      901 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
      961 ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag
     1021 acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa
     1081 ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca
     1141 tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta
     1201 ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa
     1261 acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc
     1321 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct
     1381 tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg
     1441 cgtaaagatc tggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg
     1501 atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggta
     1561 tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc
     1621 atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt
     1681 ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt
     1741 attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc agtttaaggt
     1801 ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat
     1861 tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa
     1921 agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac
     1981 caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca
     2041 ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg
     2101 ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt tgtgttttac
     2161 agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat
     2221 tttacgtttc tcgttcagct ttcttgtaca aagtggttta gtaatgaacc ggtccaccac
     2281 caccaccacc actaaggatc cggggttggg gttgcgcctt ttccaaggca gccctgggtt
     2341 tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg
     2401 gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt
     2461 gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc
     2521 ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg cagacggaca
     2581 gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata gcggctgctc
     2641 agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg ggtgtggggc
     2701 ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg
     2761 cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc agcaattcac
     2821 catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat
     2881 caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg
     2941 ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt
     3001 ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg
     3061 aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct
     3121 gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat
     3181 tcgtgaattg ctgccctctg gttatgtgtg ggagggcgaa ggtagaggtt ctctcctcac
     3241 ttgtggtgat gttgaagaaa accctggtcc aatgtctaga ctggacaaga gcaaagtcat
     3301 aaacggagct ctggaattac tcaatggtgt cggtatcgaa ggcctgacga caaggaaact
     3361 cgctcaaaag ctgggagttg agcagcctac cctgtactgg cacgtgaaga acaagcgggc
     3421 cctgctcgat gccctgccaa tcgagatgct ggacaggcat catacccact tctgccccct
     3481 ggaaggcgag tcatggcaag actttctgcg gaacaacgcc aagtcatacc gctgtgctct
     3541 cctctcacat cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag agaaacagta
     3601 cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc
     3661 actgtacgct ctgtccgccg tgggccactt tacactgggc tgcgtattgg aggaacagga
     3721 gcatcaagta gcaaaagagg aaagagagac acctaccacc gattctatgc ccccacttct
     3781 gagacaagca attgagctgt tcgaccggca gggagccgaa cctgccttcc ttttcggcct
     3841 ggaactaatc atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg ggccgaccga
     3901 cgcccttgac gattttgact tagacatgct cccagccgat gcccttgacg actttgacct
     3961 tgatatgctg cctgctgacg ctcttgacga ttttgacctt gacatgctcc ccgggtaact
     4021 aagtaaggat cgatccaaga tatcgtattc ttaactatgt tgctcctttt acgctatgtg
     4081 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     4141 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     4201 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     4261 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     4321 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     4381 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     4441 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     4501 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     4561 cgagtcggat ctccctttgg gccgcctccc cgcctgtttc gcctcggcgt ccggactaga
     4621 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     4681 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     4741 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     4801 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     4861 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     4921 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     4981 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     5041 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     5101 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     5161 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     5221 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     5281 ttttttggag gcctttcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5341 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     5401 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     5461 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5521 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5581 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     5641 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     5701 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     5761 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     5821 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     5881 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     5941 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     6001 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     6061 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     6121 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     6181 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     6241 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     6301 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     6361 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6421 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6481 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6541 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6601 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6661 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6721 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     6781 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     6841 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     6901 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     6961 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     7021 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     7081 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     7141 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     7201 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     7261 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     7321 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     7381 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
     7441 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
     7501 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
     7561 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
     7621 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg
     7681 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     7741 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     7801 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     7861 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     7921 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     7981 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     8041 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     8101 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     8161 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     8221 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     8281 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     8341 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     8401 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     8461 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     8521 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     8581 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     8641 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     8701 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     8761 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     8821 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     8881 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     8941 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     9001 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     9061 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     9121 cattatcgtt tcagacccac ctcccaaccc cga
//
