LOCUS       sequence-380715-        8766 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FATP2 (SLC27A2) protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8766)
  TITLE     Jun Liu Lab Plasmids 2022
REFERENCE   2  (bases 1 to 8766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti6MFV SLC27A2
FEATURES             Location/Qualifiers
     source          1..8766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        42..345
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        346..549
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     500..520
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     546..570
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      632..641
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             641..664
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             683..703
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             707..748
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2502..2543
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(2580..2600)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2585..2914
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2765..2900
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        2962..3009
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3028..3426
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             3513..3746
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3818..3939
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3855..3874)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3909..3928
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3979..4114
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4134..4153)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4135..4153)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4163..4180)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4163..4179)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4172..4194)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4321..4776
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4408..4427)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4618..4639
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4802..4906
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4907..4975,4976..5767)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5125..5144)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5938..6526
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6427..6446
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6680..6697
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6814..6835
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6850..6855,6856..6873,6874..6880)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6888..6904
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6893..6915
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6912..6928
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6912..6928
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6947..6967
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6949..6967
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6995..7221
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7222..7402
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7449..7574
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8067..8300
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8485..8529
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gtgaacggat ctcgacggta tcgataagct tgggagttcc gcgttacata acttacggta
       61 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      121 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      181 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      241 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      301 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      361 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      421 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      481 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      541 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
      601 ctccatagaa gacaccgact ctagaggatc cgccaccatg gattacaagg atgacgacga
      661 taagagcccg ggcggatcgt tggagaatct ttattttcag ggtcaaggta agcctatccc
      721 taaccctctc ctcggtctcg attctacgtc tagactgcag gaattcgata tcatgctttc
      781 cgccatctac acagtcctgg cgggactgct gttcctgccg ctcctggtga acctctgctg
      841 cccatacttc ttccaggaca taggctactt cttgaaggtg gccgccgtgg gccggagggt
      901 gcgcagctac gggaagcggc ggccggcgcg caccatcctg cgggcgttcc tggagaaagc
      961 gcgccagacg ccacacaagc cttttctgct cttccgcgac gagactctca cctacgcgca
     1021 ggtggaccgg cgcagcaatc aagtggcccg ggcgctgcac gaccacctcg gcctgcgcca
     1081 gggagactgc gtggcgctcc ttatgggtaa cgagccggcc tacgtgtggc tgtggctggg
     1141 gctggtgaag ctgggctgtg ccatggcgtg cctcaattac aacatccgcg cgaagtccct
     1201 gctgcactgc ttccagtgct gcggggcgaa ggtgctgctg gtgtcgccag aactacaagc
     1261 agctgtcgaa gagatactgc caagccttaa aaaagatgat gtgtccatct attatgtgag
     1321 cagaacttct aacacagatg ggattgactc tttcctggac aaagtggatg aagtatcaac
     1381 tgaacctatc ccagagtcat ggaggtctga agtcactttt tccactcctg ccttatacat
     1441 ttatacttct ggaaccacag gtgctactct tgccttgcgg actaaatttt cagccagcca
     1501 gttttgggat gactgcagaa aatacaacgt cactgtcatt cagtatatcg gtgaactgct
     1561 tcggtattta tgcaactcac cacagaaacc aaatgaccgt gatcataaag tgagactggc
     1621 actgggaaat ggcttacgag gagatgtgtg gagacaattt gtcaagagat ttggggacat
     1681 atgcatctat gagttctatg ctgccactga aggcaatatt ggatttatga attatgcgag
     1741 aaaagttggt gctgttggaa gagtaaacta cctacagaaa aaaatcataa cttatgacct
     1801 gattaaatat gatgtggaga aagatgaacc tgtccgtgat gaaaatggat attgcgtcag
     1861 agttcccaaa ggtgaagttg gacttctggt ttgcaaaatc acacaactta caccatttaa
     1921 tggctatgct ggagcaaagg ctcagacaga gaagaaaaaa ctgagagatg tctttaagaa
     1981 aggagacctc tatttcaaca gtggagatct cttaatggtt gaccatgaaa atttcatcta
     2041 tttccacgac agagttggag atacattccg gtggaaaggg gaaaatgtgg ccaccactga
     2101 agttgctgat acagttggac tggttgattt tgtccaagaa gtaaatgttt atggagtgca
     2161 tgtgccagat catgagggtc gcattggcat ggcctccatc aaaatgaaag aaaaccatga
     2221 atttgatgga aagaaactct ttcagcacat tgctgattac ctacctagtt atgcaaggcc
     2281 ccggtttcta agaatacagg acaccattga gatcactgga acttttaaac accgcaaaat
     2341 gaccctggtg gaggagggct ttaaccctgc tgtcatcaaa gatgccttgt atttcttgga
     2401 tgacacagca aaaatgtatg tgcctatgac tgaggacatc tataatgcca taagtgctaa
     2461 aaccctgaaa ctctgactcg agggggggcc cgcggttcga aggtaagcct atccctaacc
     2521 ctctcctcgg tctcgattct acgcgtaccg gttagtaatg agtttggaat taattctgtg
     2581 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     2641 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     2701 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     2761 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     2821 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     2881 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     2941 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
     3001 taatacgaca aggtgaggaa ctaaaccatg gccaagcctt tgtctcaaga agaatccacc
     3061 ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc
     3121 gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt
     3181 actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc
     3241 aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga
     3301 cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt
     3361 gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag
     3421 ggctaagcac aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga
     3481 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     3541 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     3601 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     3661 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     3721 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     3781 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     3841 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     3901 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     3961 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     4021 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     4081 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     4141 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     4201 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     4261 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     4321 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     4381 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     4441 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     4501 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     4561 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     4621 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     4681 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     4741 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     4801 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     4861 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     4921 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     4981 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5041 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5101 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     5161 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5221 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5281 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5341 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5401 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     5461 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     5521 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     5581 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     5641 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     5701 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     5761 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     5821 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     5881 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     5941 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6001 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6061 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     6121 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6181 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6241 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6301 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6361 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6421 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6481 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6541 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     6601 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     6661 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     6721 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     6781 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     6841 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     6901 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     6961 ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag
     7021 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt
     7081 gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg
     7141 ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag
     7201 tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc
     7261 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7321 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     7381 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     7441 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     7501 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     7561 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     7621 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     7681 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     7741 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     7801 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     7861 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     7921 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     7981 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     8041 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     8101 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     8161 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     8221 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     8281 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     8341 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     8401 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     8461 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     8521 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     8581 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     8641 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     8701 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     8761 cgatta
//
