LOCUS       sequence-380717-        8613 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  knockin donor vector of mAID-mCherry2-Hygro to C-terminus of 
            endogenous human eIF3b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8613)
  AUTHORS   Duan H, Zhang S, Zarai Y, Ollinger R, Wu Y, Sun L, Hu C, He Y, Tian 
            G, Rad R, Kong X, Cheng Y, Tuller T, Wolf DA
  TITLE     eIF3 mRNA selectivity profiling reveals eIF3k as a cancer-relevant 
            regulator of ribosome content.
  JOURNAL   EMBO J. 2023 Jun 15;42(12):e112362. doi: 10.15252/embj.2022112362. 
            Epub 2023 May 8.
  PUBMED    37155573
REFERENCE   2  (bases 1 to 8613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2023-06-15"; volume: "42(12):e112362. doi"; pages: " 
            10.15252/embj.2022112362. Epub 2023 May 8"
COMMENT     Sequence Label: eIF3b- pMK293 (mAID-mCherry2-Hygro) plasmid
FEATURES             Location/Qualifiers
     source          1..8613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    68..89
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(104..109,110..127,128..134)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    142..158
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     147..169
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     166..182
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     166..182
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        200..218
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     200..217
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    complement(1079..1112)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(1675..2694)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             complement(1675..2694)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     promoter        complement(2755..3254)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2803..2822)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     3196..3219
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    complement(3315..3348)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    3379..3500
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3390..3409)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3444..3463
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(3618..4328)
                     /codon_start=1
                     /product="derivative of the monomeric red fluorescent 
                     protein mCherry"
                     /label=mCherry2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFNWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQCRTMGWEASTERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVDIKLDILSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     4165..4183
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(4335..4538)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     primer_bind     complement(5462..5481)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5463..5481)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5488..5505)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5488..5504)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5497..5519)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             5642..5944
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5823..5842
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             6293..7087
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6293..7087
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6347..6366)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6957..6976
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     regulatory      7287..7296
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7293..7667
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      7805..8393
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8294..8313
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8547..8564
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
       61 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
      121 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
      181 accatgatta cgccaagcta tttaggtgac actatagaat actcaagcta tgcatcaagc
      241 ttggtaccac tagtaacggc cgccagtgtg atggatatct gcagaattcg cccttgagtc
      301 ctgtagctca gcgcgggaac agcaggcgga gtccgtcccc tggatagaca ggcatggcgg
      361 ccctaaacgg caccacctca aaaggctgag ggaccccctg aacatcgcct tggggctgac
      421 gttgagtccc gcaagaggag ccaccccggc cacagagagc tgagcccgtg tgccacccct
      481 actcgctcat ggtcccaccc tggggggaca gcaaccaggc caccctcagg caccccatta
      541 ggccttctgt cccacatgtg cccatgctgt gaggcgcgga ggcccccagg actcaggagc
      601 tcccgcactc tgctccccag cccccgactt ggaaggctct tgtctcagaa ctgctgaaac
      661 cacagtcaca gccgtgcacg ccagtgtttc tttccagtgg acacaccgcg gccgtcacta
      721 gcacaggtat caggaaatgg ccaggcatgg ccaccctcct cttcctggca cttcccaaca
      781 cctggccaca gctcctccct cacccagcac tgcctgaaag gcgccatctg caatgtggtc
      841 agagggcgca gtcgaggcca cgtcggcacc gactctacat ttcacacgag gctcagctaa
      901 ccctgggcac atgacactag ggcttcaccc ttcacccctg ccttaaagga ggtgcttcct
      961 cagtgacggg gataactgaa gcagcatgag cagccaacag gacccccatc gcgccccctg
     1021 cagacgctca ccacagtgct ccaggtcagg atcctagtga acctcttcga gggacctaat
     1081 aacttcgtat agcatacatt atacgaagtt atattaaggg ttccggatct cgaccagctt
     1141 ctgatggaat tagaacttgg caaaacaata ctgagaatga agtgtatgtg gaacagaggc
     1201 tgctgatctc gttcttcagg ctatgaaact gacacatttg gaaaccacag tacttagaac
     1261 cacaaagtgg gaatcaagag aaaaacaatg atcccacgag agatctatag atctatagat
     1321 catgagtggg aggaatgagc tggcccttaa tttggttttg cttgtttaaa ttatgatatc
     1381 caactatgaa acattatcat aaagcaatag taaagagcct tcagtaaaga gcaggcattt
     1441 atctaatccc accccacccc cacccccgta gctccaatcc ttccattcaa aatgtaggta
     1501 ctctgttctc acccttctta acaaagtatg acaggaaaaa cttccatttt agtggacatc
     1561 tttattgttt aatagatcat caatttctgc atcccgggga tctgatatca tcgatgcatg
     1621 gggtcgtgcg ctcctttcgg tcgggcgctg cgggtcgtgg ggcgggcggc tagcctattc
     1681 ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta cttctacaca
     1741 gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc
     1801 tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc
     1861 gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg cccggagccg
     1921 cggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct gctccataca
     1981 agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat ccccgaacat
     2041 cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga cattgttgga
     2101 gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc aaagcatcag
     2161 ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag tttgccagtg
     2221 atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt attgaccgat
     2281 tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg cagcgatcgc
     2341 atccatggcc tccgcgaccg gctgcagaac agcgggcagt tcggtttcag gcaggtcttg
     2401 caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc tgaattcccc
     2461 aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat aaacataacg
     2521 atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac gccctcctac
     2581 atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt cggagacgct
     2641 gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag gctttccgga
     2701 tctatccatg ctagcggtaa gcttcagctg ctcgagatct agatggatgc aggtcgaaag
     2761 gcccggagat gaggaagagg agaacagcgc ggcagacgtg cgcttttgaa gcgtgcagaa
     2821 tgccgggcct ccggaggacc ttcgggcgcc cgccccgccc ctgagcccnc ccctgagccc
     2881 gcccccggac ccaccccttc ccagcctctg agcccagaaa gcgaaggagc aaagctgcta
     2941 ttggccgctg ccccaaaggc ctacccgctt ccattgctca gcggtgctgt ccatctgcac
     3001 gagactagtg agacgtgcta cttccatttg tcacgtcctg cacgacgcga gctgcggggc
     3061 gggggggaac ttcctgacta ggggaggagt agaaggtggc gcgaaggggc caccaaagaa
     3121 cggagtcggt tggcgcctac cggtggatgt ggaatgtgtg cgaggccaga ggccacttgt
     3181 gtagcgccaa gtgcccagcg gggctgctaa agcgcatgct ccagactgcc ttgggaaaag
     3241 cgcctcccct acccggtaga atttcgaggt cgagatccta agcttggctg gacgtaaact
     3301 cctcttcaga cctaataact tcgtatagca tacattatac gaagttatat taagggttat
     3361 tgaatatgat cgacgcgtta agatacattg atgagtttgg acaaaccaca actagaatgc
     3421 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     3481 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     3541 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
     3601 atgatcagtt atctagatta cttgtacagc tcgtccatgc cgccggtgga gtggcggccc
     3661 tcggcgcgtt cgtactgttc cacgatggtg tagtcctcgt tgtgggaaag gatgtccaac
     3721 ttgatgtcga cgttgtaggc gccgggcagc tgcacgggct tcttggcctt gtaggtggtc
     3781 ttgacctcag cgtcgtagtg gccgccgtcc ttcagcttca gcctctgctt gatctcgccc
     3841 ttcagggcgc cgtcctcggg gtacatccgc tcagtggagg cctcccagcc catggtacga
     3901 cactgcatta cggggccgtc ggaggggaag ttggtgccgc gcagcttcac cttgtagatg
     3961 aactcgccgt cctgcaggga ggagtcctgg gtcacggtca ccacgccgcc gtcctcgaag
     4021 ttcatcacgc gctcccaatt gaagccctcg gggaaggaca gcttcaagta gtcggggatg
     4081 tcggcggggt gcttcacgta ggccttggag ccgtacatga actgagggga caggatgtcc
     4141 caggcgaagg gcagggggcc acccttggtc accttcagct tggcggtctg ggtgccctcg
     4201 taggggcggc cctcgccctc gccctcgatc tcgaactcgt ggccgttcac ggagccctcc
     4261 atgtgcacct tgaagcgcat gaactccttg atgatggcca tgttatcctc ctcgcccttg
     4321 ctcaccatgc tagctttata catcctcaaa tcgattttcc tcaagtacgg tgctccgtcc
     4381 attgatacct tcacgaacgc cgccgcctcc gggccaccgc ttgatttttg gcaggaaacc
     4441 atcacgttct tccggtatga tctcaccggt ggccatccca caacttgtgc cttggccgga
     4501 ggtttggctg gatctttagg acaagcactc ttctccttgg cgcctgcacc ggatcctcct
     4561 cctcctcctc cctcttggtt gccgagggga atgatttctt cagtgacgaa gaactcaatg
     4621 gtctcctctt cccaatcatc gacattgctg tccagctcgt cagtgtccac ccctgagggg
     4681 acaaagggcg cagatcaccc atgggggcac atgcctagtg gcaccgccga ggccagtggg
     4741 aaggaacgca ggagtccgcg ggcaccaggg cccaggggca gcgtaagggc cagctcctgg
     4801 ggactgaggg atctctaagt tacctcctcg caactccagg cgctcgtttt tctgctccat
     4861 atagagctcc tgggccattt tccggtactt ccggaaatct tccatcatgg tgcgccttct
     4921 ctccaccaat tcctatgcat gaggttgggg aaagcacaga actggtaaga caagtgcctc
     4981 cccatcgcga agagcccaca cccaggcgga cggtgccaga aggacagcat acagaggcac
     5041 tcaagcagga atggctagtc cattcattct cacttttccc ccaacgctgc cccccaccca
     5101 caaggcagca gtgaggtcac caactccaag tccccgtgcc aaccccagtt ccccagtgca
     5161 tccctcctgg gggggaacag aaccagtgtc ttcggagcag aggcaactct gagcacatac
     5221 acagcagctc cccgcagccc cgcctttgcc atgatggcgg atgtcacagc acagccctca
     5281 ttttgggaat gaggctcacc tttgaggctt tggactgact caaacgatcc ttctgttcaa
     5341 agatcttaga gtatttcttc agatcctttt taatttgctg aaacaaaaga cccatcactc
     5401 agaagggcga attccagcac actggcggcc gttcgagcat gcatctagag ggcccaattc
     5461 gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga
     5521 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     5581 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tatacgtacg
     5641 gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca
     5701 gagtgatatt attgacacgc cggggcgacg gatggtgatc cccctggcca gtgcacgtct
     5761 gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg
     5821 gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc
     5881 tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat
     5941 ataaatgtca ggcatgagat tatcaaaaag gatcttcacc tagatccttt tcacgtagaa
     6001 agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac
     6061 aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata
     6121 gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc
     6181 tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg
     6241 atggcgcagg ggatcaagct ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     6301 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     6361 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     6421 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
     6481 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     6541 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     6601 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     6661 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     6721 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     6781 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
     6841 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     6901 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     6961 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     7021 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     7081 cttctgaatt attaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg
     7141 cggtatttca caccgcatca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     7201 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     7261 tgcttcaata atagcacgtg aggagggcca ccatggccaa gttgaccagt gccgttccgg
     7321 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     7381 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     7441 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     7501 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     7561 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     7621 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctaaaac
     7681 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7741 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7801 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     7861 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     7921 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     7981 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8041 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8101 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8161 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8221 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8281 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8341 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8401 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8461 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     8521 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     8581 caatacgcaa accgcctctc cccgcgcgtt ggc
//
