LOCUS       sequence-380718-        8353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  knockin donor vector of mAID-mClover-NeoR to C-terminus of 
            endogenous human eIF3b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8353)
  AUTHORS   Duan H, Zhang S, Zarai Y, Ollinger R, Wu Y, Sun L, Hu C, He Y, Tian 
            G, Rad R, Kong X, Cheng Y, Tuller T, Wolf DA
  TITLE     eIF3 mRNA selectivity profiling reveals eIF3k as a cancer-relevant 
            regulator of ribosome content.
  JOURNAL   EMBO J. 2023 Jun 15;42(12):e112362. doi: 10.15252/embj.2022112362. 
            Epub 2023 May 8.
  PUBMED    37155573
REFERENCE   2  (bases 1 to 8353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2023-06-15"; volume: "42(12):e112362. doi"; pages: " 
            10.15252/embj.2022112362. Epub 2023 May 8"
COMMENT     Sequence Label: eIF3b- pMK289 (mAID-mClover-NeoR) plasmid
FEATURES             Location/Qualifiers
     source          1..8353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    44..65
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(80..85,86..103,104..110)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    118..134
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     123..145
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     142..158
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     142..158
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        176..194
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     176..193
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1098..1117
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(1305..2099)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(1305..2099)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2134..2491)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2148..2283
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        2300..2491
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     2448..2468
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(2493..2597)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2624..3079
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2711..2730)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2921..2942
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    3086..3207
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3097..3116)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3151..3170
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(3325..4038,4039..4041,4042..4044))
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from mClover2, with improved photostability (Bajar et al., 
                     2003)"
                     /label=mClover3
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(3370..3391)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3978..3999
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(4051..4254)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     primer_bind     complement(5178..5197)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5179..5197)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5204..5221)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5204..5220)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5213..5235)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             5358..5660
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5539..5558
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             6009..6803
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6009..6803
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      7003..7012
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7009..7383
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      7521..8109
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8010..8029
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8263..8280
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
       61 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
      121 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagctattta
      181 ggtgacacta tagaatactc aagctatgca tcaagcttgg taccactagt aacggccgcc
      241 agtgtgatgg atatctgcag aattcgccct tgagtcctgt agctcagcgc gggaacagca
      301 ggcggagtcc gtcccctgga tagacaggca tggcggccct aaacggcacc acctcaaaag
      361 gctgagggac cccctgaaca tcgccttggg gctgacgttg agtcccgcaa gaggagccac
      421 cccggccaca gagagctgag cccgtgtgcc acccctactc gctcatggtc ccaccctggg
      481 gggacagcaa ccaggccacc ctcaggcacc ccattaggcc ttctgtccca catgtgccca
      541 tgctgtgagg cgcggaggcc cccaggactc aggagctccc gcactctgct ccccagcccc
      601 cgacttggaa ggctcttgtc tcagaactgc tgaaaccaca gtcacagccg tgcacgccag
      661 tgtttctttc cagtggacac accgcggccg tcactagcac aggtatcagg aaatggccag
      721 gcatggccac cctcctcttc ctggcacttc ccaacacctg gccacagctc ctccctcacc
      781 cagcactgcc tgaaaggcgc catctgcaat gtggtcagag ggcgcagtcg aggccacgtc
      841 ggcaccgact ctacatttca cacgaggctc agctaaccct gggcacatga cactagggct
      901 tcacccttca cccctgcctt aaaggaggtg cttcctcagt gacggggata actgaagcag
      961 catgagcagc caacaggacc cccatcgcgc cccctgcaga cgctcaccac agtgctccag
     1021 gtcaggatcc aacgacccaa caccgtgcgt tttattctgt ctttttattg ccgtcatagc
     1081 gcgggttcct tccggtattg tctccttccg tgtttcagtt agcctccccc tagggtgggc
     1141 gaagaactcc agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac
     1201 gattccgaag cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtgatggcag
     1261 gttgggcgtc gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca
     1321 agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg
     1381 aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg
     1441 tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca
     1501 ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg
     1561 tcgggcatgc tcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct
     1621 tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg
     1681 cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc
     1741 attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc
     1801 tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc
     1861 acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcttgc
     1921 agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct
     1981 gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg
     2041 aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg
     2101 cgaaacgatc ctcatcctgt ctcttgatcg atctttgcaa aagcctaggc ctccaaaaaa
     2161 gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat
     2221 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
     2281 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat
     2341 acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg
     2401 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca
     2461 cacattccac agctggttct ttccgcctca ggactcttcc tttttcaata ttattgaagc
     2521 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     2581 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc
     2641 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     2701 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     2761 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     2821 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     2881 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     2941 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     3001 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     3061 atattaacgc ttacaattta cgcgttaaga tacattgatg agtttggaca aaccacaact
     3121 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     3181 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     3241 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
     3301 gctgattatg atcagttatc tagattactt gtacagctcg tccatgccat gtgtaatccc
     3361 ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct
     3421 cagcttggac tgatggctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat
     3481 gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcaa cgttgtggcg
     3541 gatcttgaag ttagccttga tgccgttctt ctgcttgtcg gccgtgatat agacgttgtg
     3601 gctgttgaag ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat
     3661 gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt
     3721 cttgtaggta ccgtcgtcct tgaaagagat ggtgcgctcc tggacgtagc cttcgggcat
     3781 ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcggctga agcaggccac
     3841 gccgtagccg aaggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca
     3901 gatgaacttc agggtcagct tgccgttggt ggcatcgccc tcgccctcgc cgcggacgct
     3961 gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca ccccggtgaa
     4021 cagctcctcg cccttgctca ccatgctagc tttatacatc ctcaaatcga ttttcctcaa
     4081 gtacggtgct ccgtccattg ataccttcac gaacgccgcc gcctccgggc caccgcttga
     4141 tttttggcag gaaaccatca cgttcttccg gtatgatctc accggtggcc atcccacaac
     4201 ttgtgccttg gccggaggtt tggctggatc tttaggacaa gcactcttct ccttggcgcc
     4261 tgcaccggat cctcctcctc ctcctccctc ttggttgccg aggggaatga tttcttcagt
     4321 gacgaagaac tcaatggtct cctcttccca atcatcgaca ttgctgtcca gctcgtcagt
     4381 gtccacccct gaggggacaa agggcgcaga tcacccatgg gggcacatgc ctagtggcac
     4441 cgccgaggcc agtgggaagg aacgcaggag tccgcgggca ccagggccca ggggcagcgt
     4501 aagggccagc tcctggggac tgagggatct ctaagttacc tcctcgcaac tccaggcgct
     4561 cgtttttctg ctccatatag agctcctggg ccattttccg gtacttccgg aaatcttcca
     4621 tcatggtgcg ccttctctcc accaattcct atgcatgagg ttggggaaag cacagaactg
     4681 gtaagacaag tgcctcccca tcgcgaagag cccacaccca ggcggacggt gccagaagga
     4741 cagcatacag aggcactcaa gcaggaatgg ctagtccatt cattctcact tttcccccaa
     4801 cgctgccccc cacccacaag gcagcagtga ggtcaccaac tccaagtccc cgtgccaacc
     4861 ccagttcccc agtgcatccc tcctgggggg gaacagaacc agtgtcttcg gagcagaggc
     4921 aactctgagc acatacacag cagctccccg cagccccgcc tttgccatga tggcggatgt
     4981 cacagcacag ccctcatttt gggaatgagg ctcacctttg aggctttgga ctgactcaaa
     5041 cgatccttct gttcaaagat cttagagtat ttcttcagat cctttttaat ttgctgaaac
     5101 aaaagaccca tcactcagaa gggcgaattc cagcacactg gcggccgttc gagcatgcat
     5161 ctagagggcc caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac
     5221 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     5281 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     5341 gcagcctata cgtacggcag tttaaggttt acacctataa aagagagagc cgttatcgtc
     5401 tgtttgtgga tgtacagagt gatattattg acacgccggg gcgacggatg gtgatccccc
     5461 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata
     5521 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta
     5581 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc
     5641 tgatgttctg gggaatataa atgtcaggca tgagattatc aaaaaggatc ttcacctaga
     5701 tccttttcac gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct
     5761 actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg
     5821 ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc
     5881 cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct
     5941 tgccgccaag gatctgatgg cgcaggggat caagctctga tcaagagaca ggatgaggat
     6001 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     6061 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     6121 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     6181 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     6241 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     6301 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     6361 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     6421 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     6481 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     6541 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     6601 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     6661 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     6721 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     6781 gccttcttga cgagttcttc tgaattatta acgcttacaa tttcctgatg cggtattttc
     6841 tccttacgca tctgtgcggt atttcacacc gcatcaggtg gcacttttcg gggaaatgtg
     6901 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     6961 caataaccct gataaatgct tcaataatag cacgtgagga gggccaccat ggccaagttg
     7021 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
     7081 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
     7141 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
     7201 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
     7261 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
     7321 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
     7381 tgacacgtgc taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     7441 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     7501 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     7561 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     7621 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     7681 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     7741 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     7801 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     7861 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     7921 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     7981 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     8041 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     8101 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     8161 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     8221 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     8281 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     8341 tgcagctggc acg
//
