LOCUS       sequence-380720-        7859 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses CD38 protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7859)
  TITLE     Jun Liu Lab Plasmids 2022
REFERENCE   2  (bases 1 to 7859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti6M CD38
FEATURES             Location/Qualifiers
     source          1..7859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        42..345
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        346..549
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     500..520
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     546..570
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      658..667
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1595..1636
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(1673..1693)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1678..2007
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1858..1993
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        2055..2102
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2121..2519
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             2606..2839
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2911..3032
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2948..2967)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3002..3021
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3072..3207
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3227..3246)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3228..3246)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3256..3273)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3256..3272)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3265..3287)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3414..3869
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3501..3520)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3711..3732
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3895..3999
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4000..4068,4069..4860)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4218..4237)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5031..5619
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5520..5539
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5773..5790
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5907..5928
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5943..5948,5949..5966,5967..5973)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5981..5997
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5986..6008
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6005..6021
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6005..6021
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6040..6060
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6042..6060
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6088..6314
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6315..6495
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6542..6667
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7160..7393
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7578..7622
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gtgaacggat ctcgacggta tcgataagct tgggagttcc gcgttacata acttacggta
       61 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      121 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      181 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      241 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      301 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      361 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      421 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      481 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      541 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
      601 ctccatagaa gacaccgact ctagaggatc cactagttaa ctgcagtcga cgatatcgcc
      661 accatggcca actgcgagtt cagcccggtg tccggggaca aaccctgctg ccggctctct
      721 aggagagccc aactctgtct tggcgtcagt atcctggtcc tgatcctcgt cgtggtgctc
      781 gcggtggtcg tcccgaggtg gcgccagcag tggagcggtc cgggcaccac caagcgcttt
      841 cccgagaccg tcctggcgcg atgcgtcaag tacactgaaa ttcatcctga gatgagacat
      901 gtagactgcc aaagtgtatg ggatgctttc aagggtgcat ttatttcaaa acatccttgc
      961 aacattactg aagaagacta tcagccacta atgaagttgg gaactcagac cgtaccttgc
     1021 aacaagattc ttctttggag cagaataaaa gatctggccc atcagttcac acaggtccag
     1081 cgggacatgt tcaccctgga ggacacgctg ctaggctacc ttgctgatga cctcacatgg
     1141 tgtggtgaat tcaacacttc caaaataaac tatcaatctt gcccagactg gagaaaggac
     1201 tgcagcaaca accctgtttc agtattctgg aaaacggttt cccgcaggtt tgcagaagct
     1261 gcctgtgatg tggtccatgt gatgctcaat ggatcccgca gtaaaatctt tgacaaaaac
     1321 agcacttttg ggagtgtgga agtccataat ttgcaaccag agaaggttca gacactagag
     1381 gcctgggtga tacatggtgg aagagaagat tccagagact tatgccagga tcccaccata
     1441 aaagagctgg aatcgattat aagcaaaagg aatattcaat tttcctgcaa gaatatctac
     1501 agacctgaca agtttcttca gtgtgtgaaa aatcctgagg attcatcttg cacatctgag
     1561 atctaactcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct
     1621 cggtctcgat tctacgcgta ccggttagta atgagtttgg aattaattct gtggaatgtg
     1681 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     1741 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     1801 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     1861 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     1921 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     1981 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     2041 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
     2101 acaaggtgag gaactaaacc atggccaagc ctttgtctca agaagaatcc accctcattg
     2161 aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg
     2221 cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg
     2281 gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga
     2341 cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc
     2401 gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac
     2461 agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag
     2521 cacaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     2581 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     2641 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     2701 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     2761 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     2821 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     2881 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     2941 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     3001 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     3061 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     3121 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     3181 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     3241 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     3301 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     3361 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     3421 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     3481 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     3541 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     3601 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     3661 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     3721 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     3781 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     3841 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     3901 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     3961 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     4021 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     4081 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     4141 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     4201 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     4261 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     4321 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     4381 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     4441 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     4501 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     4561 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     4621 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     4681 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     4741 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     4801 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     4861 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     4921 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     4981 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     5041 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     5101 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     5161 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     5221 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     5281 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5341 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     5401 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5461 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     5521 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     5581 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     5641 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     5701 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     5761 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     5821 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     5881 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     5941 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     6001 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     6061 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
     6121 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     6181 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     6241 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
     6301 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6361 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6421 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6481 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     6541 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     6601 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     6661 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     6721 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     6781 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     6841 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     6901 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     6961 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     7021 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     7081 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     7141 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     7201 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     7261 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     7321 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     7381 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     7441 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     7501 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     7561 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     7621 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     7681 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     7741 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
     7801 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgatta
//
