LOCUS       sequence-380738-        9078 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SGLT2 protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9078)
  TITLE     Jun Liu Lab Plasmids 2022
REFERENCE   2  (bases 1 to 9078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti6MQBI163 SGLT2
FEATURES             Location/Qualifiers
     source          1..9078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        42..345
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        346..549
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     500..520
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     546..570
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    626..788
                     /label=QBI SP163
                     /note="strong translational enhancer derived from the 5' 
                     UTR of the mouse VEGF gene"
     CDS             792..815
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(2892..2912)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2897..3226
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3077..3212
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        3274..3321
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3340..3738
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             3825..4058
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4130..4251
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4167..4186)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4221..4240
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4291..4426
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4446..4465)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4447..4465)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4475..4492)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4475..4491)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4484..4506)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4633..5088
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4720..4739)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4930..4951
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5114..5218
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5219..5287,5288..6079)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5437..5456)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6250..6838
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6739..6758
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6992..7009
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7126..7147
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7162..7167,7168..7185,7186..7192)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7200..7216
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7205..7227
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7224..7240
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7224..7240
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7259..7279
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7261..7279
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7307..7533
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7534..7714
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7761..7886
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8379..8612
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8797..8841
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gtgaacggat ctcgacggta tcgataagct tgggagttcc gcgttacata acttacggta
       61 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      121 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      181 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      241 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      301 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      361 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      421 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      481 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      541 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
      601 ctccatagaa gacaccgact ctagtagcgc agaggcttgg ggcagccgag cggcagccag
      661 gccccggccc gggcctcggt tccagaaggg agaggagccc gccaaggcgc gcaagagagc
      721 gggctgcctc gcagtccgag ccggagaggg agcgcgagcc gcgccggccc cggacggcct
      781 ccgaaaccat ggattacaaa gacgatgacg acaaggaatt catggaggag cacacagagg
      841 caggctcggc accagagatg ggggcccaga aggccctgat tgacaatcct gctgacatcc
      901 tagtcattgc tgcatatttc ctgctggtca ttggcgttgg cttgtggtcc atgtgcagaa
      961 ccaacagagg cactgtgggc ggctacttcc tggcaggacg cagcatggtg tggtggccgg
     1021 ttggggcctc tctcttcgcc agcaacatcg gcagtggcca ctttgtgggc ctggcaggga
     1081 ctggcgctgc aagtggcttg gctgttgctg gattcgagtg gaatgcgctc ttcgtggtgc
     1141 tgctactggg ctggctgttt gcacccgtgt acctgacagc gggggtcatc acgatgccac
     1201 agtacctgcg caagcgcttc ggcggccgcc gcatccgcct ctacctgtct gtgctctccc
     1261 ttttcctgta catcttcacc aagatctcag tggacatgtt ctccggagct gtattcatcc
     1321 agcaggctct gggctggaac atctatgcct ccgtcatcgc gcttctgggc atcaccatga
     1381 tttacacggt gacaggaggg ctggccgcgc tgatgtacac ggacacggta cagaccttcg
     1441 tcattctggg gggcgcctgc atcctcatgg gttacgcctt ccacgaggtg ggcgggtatt
     1501 cgggtctctt cgacaaatac ctgggagcag cgacttcgct gacggtgtcc gaggatccag
     1561 ccgtgggaaa catctccagc ttctgctatc gaccccggcc cgactcctac cacctgctcc
     1621 ggcaccccgt gaccggggat ctgccgtggc ccgcgctgct cctcggactc acaatcgtct
     1681 cgggctggta ctggtgcagc gaccaggtca tcgtgcagcg ctgcctggcc gggaagagcc
     1741 tgacccacat caaggcgggc tgcatcctgt gtgggtacct gaagctgacg cccatgtttc
     1801 tcatggtcat gccaggcatg atcagccgca ttctgtaccc agacgaggtg gcgtgcgtgg
     1861 tgcctgaggt gtgcaggcgc gtgtgcggca cggaggtggg ctgctccaac atcgcctacc
     1921 cgcggctcgt cgtgaagctc atgcccaacg gtctgcgcgg actcatgctg gcggtcatgc
     1981 tggccgcgct catgtcctcg ctggcctcca tcttcaacag cagcagcacg ctcttcacca
     2041 tggacatcta cacgcgcctg cggccacgcg ccggcgaccg cgagctgctg ctggtgggac
     2101 ggctctgggt ggtgttcatc gtggtagtgt cggtggcctg gcttcccgtg gtgcaggcgg
     2161 cacagggcgg gcagctcttc gattacatcc aggcagtctc tagctacctg gcaccgcccg
     2221 tgtccgccgt cttcgtgctg gcgctcttcg tgccgcgcgt taatgagcag ggcgccttct
     2281 ggggactcat cgggggcctg ctgatgggcc tggcacgcct gattcccgag ttctccttcg
     2341 gctcgggcag ctgtgtgcag ccctcggcgt gcccagcttt cctctgcggc gtgcactacc
     2401 tctacttcgc cattgtgctg ttcttctgct ctggcctcct caccctcacg gtctccctgt
     2461 gcaccgcgcc catccccaga aagcacctcc accgcctggt cttcagtctc cggcatagca
     2521 aggaggaacg ggaggacctg gatgctgatg agcagcaagg ctcctcactc cctgtacaga
     2581 atgggtgccc agagagtgcc atggagatga atgagcccca ggccccggca ccaagcctct
     2641 tccgccagtg cctgctctgg ttttgtggaa tgagcagagg tggggtgggc agtcctccgc
     2701 cccttaccca ggaggaggca gcggcagcag ccaggcggct ggaggacatc agcgaggacc
     2761 cgagctgggc ccgtgtggtc aacctcaatg ccctgctcat gatggcagtg gccgtgttcc
     2821 tctggggctt ctatgcctaa ctcgagtcta gagggcccac cggttagtaa tgagtttgga
     2881 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     2941 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     3001 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     3061 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     3121 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     3181 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     3241 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat
     3301 atcggcatag tataatacga caaggtgagg aactaaacca tggccaagcc tttgtctcaa
     3361 gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa
     3421 gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat
     3481 gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct
     3541 gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg
     3601 agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat caaagccata
     3661 gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt
     3721 tatgtgtggg agggctaagc acaattcgag ctcggtacct ttaagaccaa tgacttacaa
     3781 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     3841 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     3901 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     3961 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     4021 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     4081 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     4141 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     4201 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     4261 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     4321 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     4381 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     4441 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     4501 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     4561 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     4621 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     4681 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     4741 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     4801 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     4861 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     4921 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     4981 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     5041 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     5101 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5161 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     5221 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     5281 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     5341 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     5401 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     5461 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     5521 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     5581 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     5641 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     5701 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     5761 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     5821 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     5881 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     5941 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     6001 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     6061 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     6121 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6181 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6241 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6301 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6361 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     6421 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6481 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6541 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6601 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6661 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6721 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6781 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6841 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     6901 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     6961 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     7021 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     7081 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     7141 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     7201 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
     7261 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
     7321 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
     7381 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
     7441 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
     7501 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
     7561 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     7621 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     7681 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     7741 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     7801 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     7861 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     7921 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     7981 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     8041 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     8101 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     8161 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     8221 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     8281 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     8341 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     8401 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     8461 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     8521 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     8581 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     8641 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     8701 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     8761 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     8821 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     8881 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     8941 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     9001 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     9061 gacagatcca ttcgatta
//
