LOCUS       sequence-380756-       12023 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12023)
  AUTHORS   Wiznerowicz M, Trono D
  TITLE     Conditional suppression of cellular genes: lentivirus 
            vector-mediated drug-inducible RNA interference.
  JOURNAL   J Virol. 2003 Aug . 77(16):8957-61.
  PUBMED    12885912
REFERENCE   2  (bases 1 to 12023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2003 Aug . 77(16):8957-61."
COMMENT     Sequence Label: pLV-tTRKRAB-red
FEATURES             Location/Qualifiers
     source          1..12023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2145..3323
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2376..3314
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3271..3291
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3371..3488
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             3551..4171
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MARLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(3624..3643)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             4172..4192
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4295..4489
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     misc_feature    4570..5137
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4588..5139
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4732..4749)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4959..4978
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5142..5819
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(5322..5342)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     5734..5757
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    5854..6442
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5907..5927)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6325..6336)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6586..6619)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6639..6819
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6844..6862)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6845..6862)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7152..7171)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7385..7404)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7504..7526
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7564..7582)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7650..7754
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7755..7823,7824..8615)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7973..7992)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8786..9374
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9275..9294
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9528..9545
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9615..9635)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9620..9949
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9800..9935
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11083..11148
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11278..11298
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11723..11857
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11760..11779)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11814..11833
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg
     2041 accatcgatg tcgacgataa gctttgcaaa gatggataaa gttttaaaca gagaggaatc
     2101 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
     2161 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     2221 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     2281 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     2341 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg
     2401 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc
     2461 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt
     2521 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
     2581 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
     2641 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
     2701 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
     2761 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
     2821 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt
     2881 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
     2941 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag
     3001 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt
     3061 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt
     3121 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
     3181 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
     3241 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
     3301 ttcttccatt tcaggtgtcg tgaggaattt cgacatttaa atttaattaa tctcgacggt
     3361 atcggttaac ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     3421 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     3481 aaaattttat cgatcacgag actagcctcg aggtttaaac tacgggatcc aggcctaagc
     3541 ttacgcgtcc atggctagat tagataaaag taaagtgatt aacagcgcat tagagctgct
     3601 taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga
     3661 gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat
     3721 tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga
     3781 ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc
     3841 aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt
     3901 agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt
     3961 ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga
     4021 aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt
     4081 tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt
     4141 agaaaaacaa cttaaatgtg aaagtgggtc gccaaaaaag aagagaaagg tcgacggcgg
     4201 tggtgctttg tctcctcagc actctgctgt cactcaagga agtatcatca agaacaagga
     4261 gggcatggat gctaagtcac taactgcctg gtcccggaca ctggtgacct tcaaggatgt
     4321 atttgtggac ttcaccaggg aggagtggaa gctgctggac actgctcagc agatcgtgta
     4381 cagaaatgtg atgctggaga actataagaa cctggtttcc ttgggttatc agcttactaa
     4441 gccagatgtg atcctccggt tggagaaggg agaagagccc tggctggtgg agagagaaat
     4501 tcaccaagag acccatcctg attcagagac tgcatttgaa atcaaatcat cagtttaacc
     4561 cgggtaaatt ccgccccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     4621 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     4681 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     4741 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     4801 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     4861 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     4921 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     4981 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     5041 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa cgtctaggcc ccccgaacca
     5101 cggggacgtg gttttccttt gaaaaacacg atgataatac catggcctcc tccgagaacg
     5161 tcatcaccga gttcatgcgc ttcaaggtgc gcatggaggg caccgtgaac ggccacgagt
     5221 tcgagatcga gggcgagggc gagggccgcc cctacgaggg ccacaacacc gtgaagctga
     5281 aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttccagt
     5341 acggctccaa ggtgtacgtg aagcaccccg ccgacatccc cgactacaag aagctgtcct
     5401 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggcgaccg
     5461 tgacccagga ctcctccctg caggacggct gcttcatcta caaggtgaag ttcatcggcg
     5521 tgaacttccc ctccgacggc cccgtgatgc agaagaagac catgggctgg gaggcctcca
     5581 ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gacccacaag gccctgaagc
     5641 tgaaggacgg cggccactac ctggtggagt tcaagtccat ctacatggcc aagaagcccg
     5701 tgcagctgcc cggctactac tacgtggacg ccaagctgga catcacctcc cacaacgagg
     5761 actacaccat cgtggagcag tacgagcgca ccgagggccg ccaccacctg ttcctgtagc
     5821 ggccgcgact ctagaattta ctagtcatat gataatcaac ctctggatta caaaatttgt
     5881 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     5941 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     6001 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     6061 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     6121 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     6181 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     6241 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     6301 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     6361 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     6421 tccctttggg ccgcctcccc gcatcggtac gtatggccag gtacctttaa gaccaatgac
     6481 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     6541 aattcactcc caacgaagac aagatgggat caattcacca tgggaataac ttcgtatagc
     6601 atacattata cgaagttatg ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     6661 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     6721 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     6781 ccctcagacc cttttagtca gtgtggaaaa tctctagcag catctagaat taattccgtg
     6841 tattctatag tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta
     6901 gccgcgttct aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca
     6961 gaccctatca tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg
     7021 agtttctggt aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat
     7081 cgctagccag atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc
     7141 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg
     7201 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt
     7261 gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct
     7321 actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc
     7381 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     7441 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     7501 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     7561 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     7621 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     7681 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     7741 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     7801 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     7861 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     7921 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     7981 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     8041 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     8101 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     8161 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     8221 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     8281 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     8341 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     8401 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     8461 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     8521 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     8581 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     8641 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     8701 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     8761 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     8821 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     8881 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     8941 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     9001 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9061 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9121 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9181 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9241 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9301 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9361 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     9421 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     9481 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     9541 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     9601 attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     9661 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     9721 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     9781 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     9841 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     9901 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca
     9961 caagacaggc ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca
    10021 gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata
    10081 atctcgcgca acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga
    10141 cacttcacat gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta
    10201 aactcgcaag ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg
    10261 gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt
    10321 ttgtatttcc agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga
    10381 aggcgatggc gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt
    10441 tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg
    10501 tcgtccgctg gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc
    10561 gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct
    10621 ggcactgccg ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta
    10681 ttctggaggc tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct
    10741 ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg
    10801 tgcagtcggc ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga
    10861 tgtggatctg gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga
    10921 tgagcgatgc cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg
    10981 gcaactggat ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg
    11041 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
    11101 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
    11161 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
    11221 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
    11281 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
    11341 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
    11401 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
    11461 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
    11521 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
    11581 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
    11641 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
    11701 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
    11761 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    11821 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
    11881 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
    11941 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
    12001 aatatgtact acaaacttag tag
//
