LOCUS       sequence-38081-e         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Nern A, Pfeiffer BD, Svoboda K, Rubin GM
  TITLE     Multiple new site-specific recombinases for use in manipulating 
            animal genomes.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Aug 9.
  PUBMED    21831835
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2011 Aug 9."
COMMENT     Sequence Label: pJFRC177-10XUAS-FRT>-dSTOP-FRT>-myr::GFP
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    551..584
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    602..736
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(626..645)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     680..699
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gtatccctcc ggcatcgccg acttgaaaaa gtcatgctgc ttcatatgat cggggtaacg
       61 gctgaaacac tggacaccat acgtcagcgt cgtgaccaac gtcggccatg gcacgggcag
      121 cttgcccgtg gtgcatatga acttcaacgt cagcttaccg tatgtggcgt cgccctcacc
      181 ctcgccggac acactgaatt tatgaccgtt gacatcgccg tcgagttcaa ccaatatcgg
      241 gactactccg gtaaacagtt cttcaccttt ggacatggat ccgcgtggtc tgatgatgtc
      301 aacgccgccc tttccgccga ttttcaacgg accccgatga ttgggatcgg gcgtgtagcg
      361 cgagtccagc gagccaccgt tgttatgctt ctggccaaga acgccgccca tgttgccgcc
      421 gccgctgctg ttgatgtgcg tctggccaaa ggtgtagtcg gatttcttgt agcccccagt
      481 gggataggcc agtgccagtt cctgatcctg tcgcttgctg cagcatttgt tgcccatttt
      541 gattctcgag gaagttccta ttctctagaa agtataggaa cttcccggcc agatcactag
      601 tgatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
      661 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
      721 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcag
//
