LOCUS       sequence-38083-e         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Nern A, Pfeiffer BD, Svoboda K, Rubin GM
  TITLE     Multiple new site-specific recombinases for use in manipulating 
            animal genomes.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Aug 9.
  PUBMED    21831835
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2011 Aug 9."
COMMENT     Sequence Label: pJFRC172-10XUAS-loxP>-dSTOP-loxP>-myr::GFP
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(549..582)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    600..734
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(624..643)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     678..697
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 atccctccgg catcgccgac ttgaaaaagt catgctgctt catatgatcg gggtaacggc
       61 tgaaacactg gacaccatac gtcagcgtcg tgaccaacgt cggccatggc acgggcagct
      121 tgcccgtggt gcatatgaac ttcaacgtca gcttaccgta tgtggcgtcg ccctcaccct
      181 cgccggacac actgaattta tgaccgttga catcgccgtc gagttcaacc aatatcggga
      241 ctactccggt aaacagttct tcacctttgg acatggatcc gcgtggtctg atgatgtcaa
      301 cgccgccctt tccgccgatt ttcaacggac cccgatgatt gggatcgggc gtgtagcgcg
      361 agtccagcga gccaccgttg ttatgcttct ggccaagaac gccgcccatg ttgccgccgc
      421 cgctgctgtt gatgtgcgtc tggccaaagg tgtagtcgga tttcttgtag cccccagtgg
      481 gataggccag tgccagttcc tgatcctgtc gcttgctgca gcatttgttg cccattttga
      541 ttctcgagat aacttcgtat agcatacatt atacgaagtt atccggccag atcactagtg
      601 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
      661 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
      721 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
      781 tgtggga
//
