LOCUS       sequence-38094-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  TITLE     Linda Hicke lab unpublished Ubiquitin constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: LHP336 - HA-Ub-K48R
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..33
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             13..39
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             43..270
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGRQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     terminator      383..630
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(399..417)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        663..764
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
ORIGIN
        1 atgtgttacc catacgacgt tccagactac gctagcttgg gtatgcagat cttcgtcaag
       61 acgttaaccg gtaaaaccat aactctagaa gttgaatctt ccgataccat cgacaacgtt
      121 aagtcgaaaa ttcaagacaa ggaaggcatt ccacctgatc aacaaagatt gatctttgcc
      181 ggtagacagc tcgaggacgg tagaacgctg tctgattaca acattcagaa ggagtcgacc
      241 ttacatcttg tcttaagact aagaggtggt tgaggtacca agatggcctt tggtgggttg
      301 aagaaggaaa aagacagaaa cgacttaatt acctacttga aaaaagcctg tgagtaaaca
      361 ggcccctttt cctttgtcga tatcatgtaa ttagttatgt cacgcttaca ttcacgccct
      421 ccccccacat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct
      481 atttattttt ttatagttat gttagtatta agaacgttat ttatatttca aatttttctt
      541 ttttttctgt acagacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag
      601 gttttgggac gctcgaaggc tttaatttgc aagcttatcg atgataagct gtcaaacatg
      661 agaattcggt cgaaaaaaga aaaggagagg gccaagaggg agggcattgg tgactattga
      721 gcacgtgagt atacgtgatt aagcacacaa aggcagcttg gagtatgtct gttattaatt
      781 tcacaggtag ttctggtcca ttggtgaaag tttgcggctt gcagagcaca gaggccgcag
      841 aatgtgctct agattccgat gctgact
//
