LOCUS       sequence-38098-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Turowec JP, Duncan JS, French AC, Gyenis L, St Denis NA, Vilk G, 
            Litchfield DW.
  TITLE     Protein kinase CK2 is a constitutively active enzyme that promotes 
            cell survival: strategies to identify CK2 substrates and manipulate 
            its activity in mammalian cells.
  JOURNAL   Methods Enzymol. 2010;484:471-93.
  PUBMED    21036246
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2010"; volume: "484"; pages: "471-93"
COMMENT     Sequence Label: pZW12 (CK2beta)
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     74..90
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     74..90
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             107..136
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(843..861)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(844..861)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(881..898)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 cgagctcgga tccactagta acggccgcca gtgtgctgga attctgcaga tatccatcac
       61 actgggcggc cgctctagaa ctagtggatc caccatggcg tctatggaac aaaagctgat
      121 tagcgaagag gacctgaaca acgggaattc tcgagcagcc gctgacgtga agatgagcag
      181 ctcagaggag gtgtcctgga tttcctggtt ctgtgggctc cgtggcaatg aattcttctg
      241 tgaagtggat gaagactaca tccaggacaa atttaatctt actggactca atgagcaggt
      301 ccctcactat cgacaagctc tagacatgat cttggacctg gagcctgatg aagaactgga
      361 agacaacccc aaccagagtg acctgattga gcaggcagcc gagatgcttt atggattgat
      421 ccacgcccgc tacatcctta ccaaccgtgg catcgcccag atgttggaaa agtaccagca
      481 aggagacttt ggttactgtc ctcgtgtgta ctgtgagaac cagccaatgc ttcccattgg
      541 cctttcagac atcccaggtg aagccatggt gaagctctac tgccccaagt gcatggatgt
      601 gtacacaccc aagtcatcaa gacaccatca cacggatggc gcctacttcg gcactggttt
      661 ccctcacatg ctcttcatgg tgcatcccga gtaccggccc aagagacctg ccaaccagtt
      721 tgtgcccagg ctctacggtt tcaagatcca tccgatggcc taccagctgc agctccaagc
      781 cgccagcaac ttcaagagcc cagtcaagac gattcgctga ttccctcgag ggggggccct
      841 attctatagt gtcacctaaa tgctagagct cgctgatcag cctcgactgt gccttctagt
//
