LOCUS       sequence-38099-e        1631 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Turowec JP, Duncan JS, French AC, Gyenis L, St Denis NA, Vilk G, 
            Litchfield DW.
  TITLE     Protein kinase CK2 is a constitutively active enzyme that promotes 
            cell survival: strategies to identify CK2 substrates and manipulate 
            its activity in mammalian cells.
  JOURNAL   Methods Enzymol. 2010;484:471-93.
  PUBMED    21036246
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2010"; volume: "484"; pages: "471-93"
COMMENT     Sequence Label: pGV15 (CK2alpha K68M)
FEATURES             Location/Qualifiers
     source          1..1631
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..26
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10..26
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             1295..1384
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     promoter        complement(1453..1471)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1454..1471)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1491..1508)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1497..1608
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ggcggccgct ctagaactag tggatcctct gaaacaaggt cttaccccca gctgcttctg
       61 aacacagtga ctgccagatc tccaaacatc aagtccagct ttgtccgcca acctgtctga
      121 catgtcggga cccgtgccaa gcagggccag agtttacaca gatgttaata cacacagacc
      181 tcgagaatac tgggattacg agtcacatgt ggtggaatgg ggaaatcaag atgactacca
      241 gctggttcga aaattaggcc gaggtaaata cagtgaagta tttgaagcca tcaacatcac
      301 aaataatgaa aaagttgttg ttatgattct caagccagta aaaaagaaga aaattaagcg
      361 tgaaataaag attttggaga atttgagagg aggtcccaac atcatcacac tggcagacat
      421 tgtaaaagac cctgtgtcac gaacccccgc cttggttttt gaacacgtaa acaacacaga
      481 cttcaagcaa ttgtaccaga cgttaacaga ctatgatatt cgattttaca tgtatgagat
      541 tctgaaggcc ctggattatt gtcacagcat gggaattatg cacagagatg tcaagcccca
      601 taatgtcatg attgatcatg agcacagaaa gctacgacta atagactggg gtttggctga
      661 gttttatcat cctggccaag aatataatgt ccgagttgct tcccgatact tcaaaggtcc
      721 tgagctactt gtagactatc agatgtacga ttatagtttg gatatgtgga gtttgggttg
      781 tatgctggca agtatgatct ttcggaagga gccatttttc catggacatg acaattatga
      841 tcagttggtg aggatagcca aggttctggg gacagaagat ttatatgact atattgacaa
      901 atacaacatt gaattagatc cacgtttcaa tgatatcttg ggcagacact ctcgaaagcg
      961 atgggaacgc tttgtccaca gtgaaaatca gcaccttgtc agccctgagg ccttggattt
     1021 cctggacaaa ctgctgcgat atgaccacca gtcacggctt actgcaagag aggcaatgga
     1081 gcacccctat ttctacactg ttgtgaagga ccaggctcga atgggttcat ctagcatgcc
     1141 agggggcagt acgcccgtca gcagcgccaa tatgatgtca gggatttctt cagtgccaac
     1201 cccttcaccc cttggacctc tggcaggctc accagtgatt gctgctgcca acccccttgg
     1261 gatgcctgtt ccagctgccg ctggcgctca gcagtaccca tacgatgttc ctgactatgc
     1321 gggctatccc tatgacgtcc cggactatgc aggatcctat ccatatgacg ttccagatta
     1381 cgcttaaaag ccgaattctg cagatatcca tcacactggc ggccgctcga gcatgcatct
     1441 agagggccct attctatagt gtcacctaaa tgctagagct cgctgatcag cctcgactgt
     1501 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     1561 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     1621 taggtgtcat t
//
