LOCUS       sequence-381012-        8134 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 PhiC31 cassette with LR dvp promoter : 
            pLBD16-PhiC31-DST-tUBQ1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8134)
  AUTHORS   Guiziou S, Maranas CJ, Chu JC, Nemhauser JL
  TITLE     An integrase toolbox to record gene-expression during plant 
            development.
  JOURNAL   Nat Commun. 2023 Apr 3;14(1):1844. doi: 10.1038/s41467-023-37607-5.
  PUBMED    37012288
REFERENCE   2  (bases 1 to 8134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-3"; volume: "14(1):1844. doi"; pages: " 
            10.1038/s41467-023-37607-5"
COMMENT     Sequence Label: L1P96
FEATURES             Location/Qualifiers
     source          1..8134
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    44..68
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(159..747)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(239..258)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(918..1733)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1641..1660
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1725..1744
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      2024..2459
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    2594..2616
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     polyA_signal    2624..2800
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(3010..4035)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(4052..4397)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(4089..4109)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     4604..4626
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4618..4635
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4619..4635
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4642..4661
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4642..4660
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5669..7486
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
ORIGIN
        1 accacagaat caggggataa cgcaggaaag aacatgaagg ccttgacagg atatattggc
       61 gggtaaacta agtcgctgta tgtgtttgtt tgagatctca tgtgagcaaa aggccagcaa
      121 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
      181 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
      241 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
      301 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
      361 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
      421 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      481 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
      541 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
      601 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaagaag agttggtagc
      661 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
      721 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
      781 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
      841 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgtg
      901 taacattggt ctagtgatta gaaaaactca tcgagcatca aatgaaactg caatttattc
      961 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac
     1021 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt
     1081 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa
     1141 tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag
     1201 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg
     1261 ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa
     1321 ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt
     1381 tcacctgaat caggatattc ttctaatacc tggaatgctg ttttccctgg gatcgcagtg
     1441 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata
     1501 aattccgtca gccagtttag tctgaccatc tcatctgtaa caacattggc aacgctacct
     1561 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg gtagattgtc
     1621 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg
     1681 ttggaattta atcgcggcct tgagcaagac gtttcccgtt gaatatggct cataacaccc
     1741 cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct
     1801 tgtgcaatgt aacatcagag attttgagac acaacgtggc tttgttgaat aaatcgaact
     1861 tttgctgagt tgaaggatca gatcacgcat cttcccgaca acgcagaccg ttccgtggca
     1921 aagcaaaagt tcaaaatcac caactggtcc acctacaaca aagctctcat caaccgtggc
     1981 tccctcactt tctggctgga tgatggggcg attcaggcga tccccatcca acagcccgcc
     2041 gtcgagcggg cttttttatc cccggaagcc tgtggataga gggtagttat ccacgtgaaa
     2101 ccgctaatgc cccgcaaagc cttgattcac ggggctttcc ggcccgctcc aaaaactatc
     2161 cacgtgaaat cgctaatcag ggtacgtgaa atcgctaatc ggagtacgtg aaatcgctaa
     2221 taaggtcacg tgaaatcgct aatcaaaaag gcacgtgaga acgctaatag ccctttcaga
     2281 tcaacagctt gcaaacaccc ctcgctccgg caagtagtta cagcaagtag tatgttcaat
     2341 tagcttttca attatgaata tatatatcaa ttattggtcg cccttggctt gtggacaatg
     2401 cgctacgcgc accggctccg cccgtggaca accgcaagcg gttgcccacc gtcgagcgcc
     2461 agcgcctttg cccacaaccc ggcggccggc cgcaacagat cgttttataa attttttttt
     2521 ttgaaaaaga aaaagcccga aaggcggcaa cctctcgggc ttctggattt ccgatccccg
     2581 gaattagaga tcttggcagg atatattgtg gtgtaacgtt atcgatctgg attttagtac
     2641 tggattttgg ttttaggaat tagaaatttt attgatagaa gtattttaca aatacaaata
     2701 catactaagg gtttcttata tgctcaacac atgagcgaaa ccctataaga accctaattt
     2761 cccttatcgg gaaactactc acacattatt tatggagaaa atagagagag atagatttgt
     2821 agagagagac tggtgatttc agcgtaccga attaattctc ccgcgaccag ccgagcgagc
     2881 ttagcgaact gtggacgaga actgtgccac caagcgtaag gccgttctct cgcatttgcc
     2941 ttgctaggct cgcgcgagtt gctggctgag gcgttctcga aatcagctct tgttcggtcg
     3001 gcatctactc tattcctttg ccctcggacg agtgctgggg cgtcggtttc cactatcggc
     3061 gagtacttct acacagccat cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg
     3121 cccgacagtc ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc
     3181 atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa tgcggagcat
     3241 atacgcccgg aggcgtggcg atcctgcaag ctccggatgc ctccgctcga agtagcgcgt
     3301 ctgctgctcc atacaagcca accacggcct ccagaagaag atgttggcga cctcgtattg
     3361 ggaatccccg aacatcgcct cgctccagtc aatgaccgct gttatgcggc cattgtccgt
     3421 caggacattg ttggagccga aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc
     3481 ggcccaaagc atcagctcat cgagagcctg cgcgacggac gcactgacgg tgtcgtccat
     3541 cacagtttgc cagtgataca catggggatc agcaatcgcg catatgaaat cacgccatgt
     3601 agtgtattga ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc
     3661 ggccgcagcg atcgcatcca tagcctccgc gaccggctga agaacagcgg gcagttcggt
     3721 ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg gagatgcaat aggtcaggct
     3781 ctcgctgaac tccccaatgt caagcacttc cggaatcggg agcgcggccg atgcaaagtg
     3841 ccgataaaca taacgatctt tgtagaaacc atcggcgcag ctatttaccc gcaggacata
     3901 tccacgccct cctacatcga agctgaaagc acgagattct tcgccctccg agagctgcat
     3961 caggtcggag acgctgtcga acttttcgat cagaaacttc tcgacagacg tcgcggtgag
     4021 ttcaggcttt ttcataggga tcagcttggg ctgtcctctc caaatgaaat gaacttcctt
     4081 atatagagga agggtcttgc gaaggatagt gggattgtgc gtcatccctt acgtcagtgg
     4141 agatgtcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga
     4201 tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttgaatga
     4261 tagcctttcc tttatcgcaa tgatggcatt tgtaggagcc accttccttt tctactgtcc
     4321 tttcgatgaa gtgacagata gctgggcaat ggaatccgag gaggtttccc gaaattaccc
     4381 tttgttgaaa agtctcaata gccctttggt cttctgagac tgtatctttg acatttttgg
     4441 agtaggggta cgataacatt aacgtttaca atttcgcgcc attcgccatt caggctgcgc
     4501 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     4561 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     4621 aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcttggagcc attattgatt
     4681 gtctccttag aatccaacat gtgaccctcc attgtctacc attcacaccc tttggctcac
     4741 tcgataaatt tggacattaa ttgcattcat tttatttgta agaaccagaa tctcagtaga
     4801 tgttatttgt gttgagtaaa ctgttgttat ttatagacta ggctattctt cgtatacact
     4861 ttgatactga tttttagttc atcatctatc taccgactaa atatagtgtt tctatgtggc
     4921 ttctcctcaa atgtcaaatt tatttcttta atgttgtttg agtttcaaac ttgcaaagtg
     4981 caaaccaatt ttggttgtta tactaaaacc acacatataa ttaaagacac ccactatagc
     5041 ataaaattcg tcgtgtaagc aattttacta tagtctaaac tccataaaag gacgtcgaaa
     5101 aatattgtga tattggaatt atagtgtcag tttgtttttc tttgggcaaa tggattgtag
     5161 tttcattata tattacaatt tataaacgaa acgataagta tggctcatac tttagtgatg
     5221 gagaattaaa gtattttata ttcagagatg acacaatatg gtagaccaaa aataactaat
     5281 taaaatagta tcatattaga tatttatatg tgattgtaaa tttgatcatt gagtgaacat
     5341 tttaaattag tatgcccaag aaagaaaaga aaaaaaaaaa actctttctc tctcatgata
     5401 atgttccttt tataaaatcc acccaaagac accaaacact tctccatcat catttcattt
     5461 cttgttcttt ttccaccatc accatttcct agttcgctcg tgcacatctc cgacgatccc
     5521 aattctaaaa tcatcacttc ttccaatctt ttcaacatct cttcaaaata tccagtcaac
     5581 aaaaaaaaaa gtacttaaaa caaggaaacc ccttctttct ttttgtacat ataacataaa
     5641 aatcatcact tttttgttcg tttcgccgat ggacacgtat gccggcgctt atgatagaca
     5701 atcaagagaa agagagaact cttctgccgc ctctccagct acacagagat ctgccaacga
     5761 ggataaagct gctgatttac aaagagaggt agagagagat gggggtaggt ttagatttgt
     5821 gggtcatttc tctgaggctc ctggtacatc agcctttggt actgctgaac ggcctgaatt
     5881 tgaaagaata ttaaatgagt gtcgggccgg tcgactaaat atgataatag tttatgatgt
     5941 atctaggttc agtagactca aagttatgga tgcaattccg atcgtgagcg agctacttgc
     6001 actcggtgtt actatcgtaa gtacacaaga aggtgtattc aggcagggta atgtgatgga
     6061 ccttatacac cttattatgc gactcgacgc tagccataaa gagtcatcac tgaagagtgc
     6121 taaaatcttg gacaccaaga acctgcaaag ggagcttgga ggctatgtag gaggaaaagc
     6181 tccgtatggt ttcgaactcg tgagtgagac aaaagagatt accagaaatg gtaggatggt
     6241 gaacgtggtt attaataagc tagctcatag cacaacccct ctcacagggc cttttgagtt
     6301 cgaacctgac gttattagat ggtggtggag agagatcaaa actcacaaac atcttccatt
     6361 taagccaggt agccaggctg ctatccaccc tggatctatc actgggcttt gtaaaaggat
     6421 ggatgctgac gcagtgccaa ctcgagggga aacaatcggt aagaagactg catcttccgc
     6481 ttgggatccc gctacagtta tgagaatcct tcgggatccg cgtattgctg gattcgccgc
     6541 agaagtcatt tataagaaaa aacctgatgg tactcctact acaaaaatcg aaggttatag
     6601 gatccaaagg gatcctatta ctttgcgtcc agttgaatta gactgtggac cgatcatcga
     6661 acctgccgag tggtacgagt tgcaggcctg gcttgatggt agagggcgtg gaaagggttt
     6721 atctcgggga caggcaattt tgtcagcaat ggataagctt tattgtgagt gtggggctgt
     6781 tatgacatca aaacgtggag aagagtcaat caaggattct tacaggtgca gacgtcgtaa
     6841 agtggtggac ccttctgctc caggacagca tgagggtaca tgcaacgttt ctatggctgc
     6901 tcttgataaa tttgtggccg aacgtatttt caacaagatt agacacgctg aaggggatga
     6961 ggaaacttta gctcttcttt gggaggctgc ccggcgtttt ggtaagctaa cagaggcacc
     7021 agaaaaaagt ggcgaaagag ctaatttggt agccgaaaga gcagacgcct tgaatgcatt
     7081 ggaagaactt tacgaagata gggctgccgg agcatatgat ggaccagttg gtcggaagca
     7141 tttcaggaag caacaagctg cactaacttt gagacagcaa ggcgcagagg agcgactcgc
     7201 agagcttgag gccgccgagg ccccaaagct tccactcgat caatggtttc ctgaggatgc
     7261 cgacgctgat cctacaggtc caaagagctg gtggggaaga gctagcgtag acgataagag
     7321 agtgtttgtg ggtctatttg ttgacaagat tgtagttact aagtccacta cgggtcgagg
     7381 acagggcact cccatcgaaa agagagccag tattacctgg gcaaagcctc caaccgatga
     7441 tgatgaagac gatgcacaag atggtaccga ggatgtggca gcataataag cttagtacta
     7501 tactacaaca tttccatatt ttttttagat tgttagctaa tttcccctgg agataattgt
     7561 aaattgtttc aatgagagga atatacaata catagatcgt aattgatcaa tgcgtatttg
     7621 catgttggta agagactctt atcaagaatc ccatctcttg cttgcttttt tttgttgtct
     7681 tccctttgat agggtttgtt tttcttgttt cagtgacttt ctatgttaaa agataatgtc
     7741 agtaaaagga tttggttttc tattattctg aatcgattac ggaagattct tgcttaattc
     7801 caatctatac aagtatcgtg aaataatgac cgtttatgtg attaggagac gtgtttcatt
     7861 aataaaatat aagatcaata cattgttagt agtgataaac tatgtacaaa ttgtattgat
     7921 tgtaaaagaa acacaatagg ttcctttttt ctacaatata ttgtgacaga ctctctgttt
     7981 taacgaatga attaaatttg tcaacgaaaa aattaaagaa tgatgtgatc actttgcacc
     8041 ttggttcatc ttcagaggct tctgcatgtt ccatgcattg aggctctcta cggtcacagg
     8101 ttgtgagccg ttgttaaaga caaacaaatg cgct
//
