LOCUS       sequence-38103-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent viral expression of oChIEF-tdTomato for optogenetic 
            excitation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Bell KA, Shim H, Chen CK, McQuiston AR
  TITLE     Nicotinic excitatory postsynaptic potentials in hippocampal CA1 
            interneurons are predominantly mediated by nicotinic receptors that 
            contain alpha4 and beta2 subunits.
  JOURNAL   Neuropharmacology. 2011 Aug 25.
  PUBMED    21878344
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuropharmacology. 2011 Aug 25."
COMMENT     Sequence Label: AAV-Flex-rev-oChIEF-tdTomato
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..101
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 tcgatggtga gatctggact agagggtcga tggtgatgct tggatcaaaa taggaagaat
       61 cccaatgata acttcgtata aagtatccta tacgaagtta tactagaatt cggtaccacc
      121 ggtgaccatg gtgagcagaa gaccctggct gctggccctg gccctggccg tggccctggc
      181 cgccggcagc gccggcgcca gcaccggcag cgacgccacc gtgcccgtgg ccacccagga
      241 cggccccgac tacgtgttcc acagagccca cgagagaatg ctgttccaga ccagctacac
      301 cctggagaac aacggcagcg tgatctgcat ccccaacaac ggccagtgct tctgcctggc
      361 ctggctgaag agtaacggca ccaacgccga gaagctggcc gccaacatcc tgcagtggat
      421 caccttcgcc ctgagcgccc tgtgcctgat gttctacggc taccagacct ggaagagtac
      481 ctgcggctgg gaggagatct acgtggccac catcgagatg atcaagttca tcatagagta
      541 cttccacgag ttcgacgagc ccgccgtgat ctacagcagc aacggcaaca agaccgtgtg
      601 gctgagatac gccgagtggc tgctgacctg ccccgtggtc ctgatccacc tgagcaacct
      661 gaccggcctg gccaacgact acaacaagag aaccatgggc ctgctggtga gcgacatcgg
      721 caccatcgtg tggggcacca ccgccgccct gagcaagggc tacgtgagag tgatcttctt
      781 cctgatgggc ctgtgctacg gcatctacac cttcttcaac gccgccaagg tgtacatcga
      841 ggcctacca
//
