LOCUS       sequence-38104-e         666 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent viral expression of oChIEF-tdTomato for optogenetic 
            excitation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Bell KA, Shim H, Chen CK, McQuiston AR
  TITLE     Nicotinic excitatory postsynaptic potentials in hippocampal CA1 
            interneurons are predominantly mediated by nicotinic receptors that 
            contain alpha4 and beta2 subunits.
  JOURNAL   Neuropharmacology. 2011 Aug 25.
  PUBMED    21878344
REFERENCE   2  (bases 1 to 666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuropharmacology. 2011 Aug 25."
COMMENT     Sequence Label: AAV-Flex-rev-oChIEF-tdTomato
FEATURES             Location/Qualifiers
     source          1..666
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    65..98
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    169..202
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 aattttgtat ccagaggttg attatcgata agcttgatat cgaattcgaa cctcttcgag
       61 ggacataact tcgtataatg tatgctatac gaagttatgg gtcgatggtg agatctggac
      121 tagagggtcg atggtgatgc ttggatcaaa ataggaagaa tcccaatgat aacttcgtat
      181 aaagtatcct atacgaagtt atactagaat tcggtaccac cggtgaccat ggtgagcaga
      241 agaccctggc tgctggccct ggccctggcc gtggccctgg ccgccggcag cgccggcgcc
      301 agcaccggca gcgacgccac cgtgcccgtg gccacccagg acggccccga ctacgtgttc
      361 cacagagccc acgagagaat gctgttccag accagctaca ccctggagaa caacggcagc
      421 gtgatctgca tccccaacaa cggccagtgc ttctgcctgg cctggctgaa gagtaacggc
      481 accaacgccg agaagctggc cgccaacatc ctgcagtgga tcaccttcgc cctgagcgcc
      541 ctgtgcctga tgttctacgg ctaccagacc tggaagagta cctgcggctg ggaggagatc
      601 tacgtggcca ccatcgagat gatcaagttc atcatagagt acttccacga gttcgacgag
      661 cccgcc
//
