LOCUS       sequence-38105-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  TITLE     Linda Hicke lab unpublished Ubiquitin constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: LHP938 - Gal1-HA-Ub
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             101..127
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             107..133
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             137..364
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             137..364
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     terminator      477..724
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
ORIGIN
        1 tctagcagct gtatacgact cactataggg aatattaagc ttggtaccga gctcggatcc
       61 actagtaacg gccgccagtg tgctggaatt cattatgtgt tacccatacg acgttccaga
      121 ctacgctagc ttgggtatgc agatcttcgt caagacgtta accggtaaaa ccataactct
      181 agaagttgaa tcttccgata ccatcgacaa cgttaagtcg aaaattcaag acaaggaagg
      241 cattccacct gatcaacaaa gattgatctt tgccggtaag cagctcgagg acggtagaac
      301 gctgtctgat tacaacattc agaaggagtc gaccttacat cttgtcttaa gactaagagg
      361 tggttgaggt accaagatgg cctttggtgg gttgaagaag gaaaaagaca gaaacgactt
      421 aattacctac ttgaaaaaag cctgtgagta aacaggcccc ttttcctttg tcgatatcat
      481 gtaattagtt atgtcacgct tacattcacg ccctcccccc acatccgctc taaccgaaaa
      541 ggaaggagtt agacaacctg aagtctaggt ccctatttat ttttttatag ttatgttagt
      601 attaagaacg ttatttatat ttcaaatttt tctttttttt ctgtacagac gcgtgtacgc
      661 atgtaacatt atactgaaaa ccttgcttga gaaggttttg ggacgctcga aggctttaat
      721 ttgcaagctt atcgatgata agctgtcaaa catgagaatt ctgcagatat ccatcacact
      781 ggcggccgct cgagcatgca tctagagggc cgcatcatgt aattagttat gtcacgctta
      841 cattcac
//
