LOCUS       sequence-381060-        9003 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses CreN59-nMag-NLS with mCherry marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9003)
  AUTHORS   Allen ME, Zhou W, Thangaraj J, Kyriakakis P, Wu Y, Huang Z, Ho P, 
            Pan Y, Limsakul P, Xu X, Wang Y
  TITLE     An AND-Gated Drug and Photoactivatable Cre-loxP System for 
            Spatiotemporal Control in Cell-Based Therapeutics.
  JOURNAL   ACS Synth Biol. 2019 Oct 18;8(10):2359-2371. doi: 
            10.1021/acssynbio.9b00175. Epub 2019 Oct 8.
  PUBMED    31592660
REFERENCE   2  (bases 1 to 9003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00175"; journalName: "ACS Synth Biol"; date: 
            "2019-10-18- 18"; volume: "8"; issue: "10"; pages: "2359-2371"
COMMENT     Sequence Label: pSin_EF1a_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR
FEATURES             Location/Qualifiers
     source          1..9003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             138..771
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    818..943
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1436..1669
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1854..1898
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2185..2302
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2431..3609
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2662..3600
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3557..3577
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4235..4255
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4271..4327
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4334..5041
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(4476..4494)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4744..4763
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5141..5713
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5307..5324)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5534..5553
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5746..6345
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5746..5765)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6242..6262
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6548..6781
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6932..6949)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6938..7162
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      7205..7214
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        7258..7362
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7363..7431,7432..8223)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7581..7600)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8394..8982
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8883..8902
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttacggttct ggccttttgc acggaccgcc actgccaatt acctgtggtt tcatttactc
       61 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
      121 actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga
      181 tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca caccagggcc
      241 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga
      301 taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca
      361 tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt
      421 tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg
      481 ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga
      541 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct
      601 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
      661 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
      721 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
      781 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
      841 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      901 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      961 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     1021 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     1081 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     1141 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     1201 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     1261 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     1321 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1381 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1441 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1501 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1561 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1621 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1681 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1741 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     1801 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     1861 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     1921 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     1981 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     2041 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     2101 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc
     2161 acaaatggca gtattcatcc acaattttaa aagaaagggg gggattgggg ggtacagtgc
     2221 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2281 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggat
     2341 cgataagctt tgcaaagatg gataaagttt taaacagaga ggaatctttg cagctaatgg
     2401 accttctagg tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc
     2461 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag
     2521 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     2581 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     2641 gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt
     2701 acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt
     2761 gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc
     2821 ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc
     2881 tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt
     2941 tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat
     3001 ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag
     3061 cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag
     3121 tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc
     3181 tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc
     3241 ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag
     3301 tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg
     3361 gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct
     3421 ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact
     3481 gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt
     3541 ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag
     3601 gtgtcgtgag gaattgatcc ttcgaactag tatggccacc tctgatgaag tcaggaagaa
     3661 cctgatggac atgttcaggg acaggcaggc cttctctgaa cacacctgga agatgctcct
     3721 gtctgtgtgc agatcctggg ctgcctggtg caagctgaac ggtacccata ctctttatgc
     3781 ccccggtgga tatgacatta tgggatatct ggaccagatc ggcaaccggc caaacccgca
     3841 ggtggaactg ggccccgtgg atacatcctg cgccttgatt ctttgtgacc tgaaacagaa
     3901 agacaccccg atagtttacg cgagtgaagc cttcctctac atgacaggtt acagcaacgc
     3961 agaggtgctg ggccggaatt gccggtttct gcaaagccct gacggcatgg tgaagcccaa
     4021 gagcacccgg aagtacgtgg atagtaacac aatcaatact atgcgcaagg caatcgacag
     4081 gaatgccgag gtgcaggttg aagtagtcaa ttttaaaaag aatggacagc gatttgttaa
     4141 tttcctgact atgatacctg ttagggacga aacaggcgag tatcgatact ctatgggatt
     4201 ccagtgcgaa acagaaggcg gaagcggtgg cgtgcccaag aagaagagga aagtcggatc
     4261 cggcagcggc gccaccaact tcagcctgct gaagcaggcc ggcgacgtgg aggagaaccc
     4321 cggccccacg cgtgtgagca agggcgagga ggataacatg gccatcatca aggagttcat
     4381 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     4441 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     4501 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
     4561 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     4621 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     4681 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     4741 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     4801 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
     4861 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     4921 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     4981 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta
     5041 ataggaattc gaagcttgag ctcgagatct gagtccggta gcgctagttc gaaggatccg
     5101 catgcatcta gggcggccaa ttccgcccct ctcccccccc cccctctccc yccccccccc
     5161 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     5221 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     5281 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     5341 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     5401 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     5461 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     5521 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     5581 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
     5641 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     5701 aacacgatga taagcttgcc acaacccaca aggagacgac cttccatgac cgagtacaag
     5761 cccacggtgc gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc
     5821 gcgttcgccg actaccccgc cacgcgccac accgtcgacc cggaccgcca catcgagcgg
     5881 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     5941 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     6001 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     6061 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     6121 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     6181 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     6241 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     6301 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgataata ggcggccgct
     6361 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     6421 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
     6481 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     6541 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     6601 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6661 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6721 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     6781 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     6841 gagagtgaga ggccttgaca ttataataga tttagcagga attgaactag gagtggagca
     6901 cacaggcaaa gttctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca
     6961 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     7021 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     7081 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     7141 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg gcgcgcccct
     7201 cgaggccgcc atggtcatag ctgtttgacg tcaggtggca cttttcgggg aaatgtgcgc
     7261 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct cacgagacaa
     7321 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     7381 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     7441 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     7501 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     7561 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     7621 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     7681 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     7741 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     7801 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     7861 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     7921 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     7981 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     8041 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     8101 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     8161 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     8221 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     8281 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     8341 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     8401 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     8461 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     8521 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     8581 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     8641 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     8701 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     8761 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     8821 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     8881 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     8941 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     9001 ttt
//
