LOCUS       sequence-381061-        9712 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses NLS-pMag-CreC60 with tagBFP marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9712)
  AUTHORS   Allen ME, Zhou W, Thangaraj J, Kyriakakis P, Wu Y, Huang Z, Ho P, 
            Pan Y, Limsakul P, Xu X, Wang Y
  TITLE     An AND-Gated Drug and Photoactivatable Cre-loxP System for 
            Spatiotemporal Control in Cell-Based Therapeutics.
  JOURNAL   ACS Synth Biol. 2019 Oct 18;8(10):2359-2371. doi: 
            10.1021/acssynbio.9b00175. Epub 2019 Oct 8.
  PUBMED    31592660
REFERENCE   2  (bases 1 to 9712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00175"; journalName: "ACS Synth Biol"; date: 
            "2019-10-18- 18"; volume: "8"; issue: "10"; pages: "2359-2371"
COMMENT     Sequence Label: pSin_EF1a_tagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR
FEATURES             Location/Qualifiers
     source          1..9712
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             140..773
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    820..945
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1438..1671
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1856..1900
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2187..2304
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2433..3611
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2664..3602
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3559..3579
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3634..4332
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             4348..4404
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4414..4434
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    join(5851..6425,6426..6428,6429..6437)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6018..6035)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6245..6264
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6457..7056
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6457..6476)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6953..6973
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7259..7492
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7643..7660)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7649..7873
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      7916..7925
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        7969..8073
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8074..8142,8143..8934)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8292..8311)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9105..9693
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9594..9613
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttttacggtt ctggcctttt gcacggaccg ccactgccaa ttacctgtgg tttcatttac
       61 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat
      121 gtactacaaa cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt
      181 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg
      241 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca
      301 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg
      361 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca
      421 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct
      481 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg
      541 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct
      601 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      661 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      721 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      781 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      841 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      901 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      961 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     1021 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     1081 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     1141 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     1201 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     1261 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     1321 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1381 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1441 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1501 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1561 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1621 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1681 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1741 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1801 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1861 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1921 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1981 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     2041 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     2101 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     2161 ccacaaatgg cagtattcat ccacaatttt aaaagaaagg gggggattgg ggggtacagt
     2221 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     2281 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg
     2341 atcgataagc tttgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat
     2401 ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc
     2461 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct
     2521 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
     2581 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
     2641 acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct
     2701 ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc
     2761 ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag
     2821 ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa
     2881 tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt
     2941 tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag
     3001 atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc
     3061 agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt
     3121 agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc
     3181 cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc
     3241 ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg
     3301 agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca
     3361 cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt
     3421 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga
     3481 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt
     3541 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc
     3601 aggtgtcgtg aggaattgat ccttcgaact agtatgagcg agctgattaa ggagaacatg
     3661 cacatgaagc tgtacatgga gggcaccgtg gacaaccatc acttcaagtg cacatccgag
     3721 ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggtggt cgagggcggc
     3781 cctctcccct tcgccttcga catcctggct actagcttcc tctacggcag caagaccttc
     3841 atcaaccaca cccagggcat ccccgacttc ttcaagcagt ccttccctga gggcttcaca
     3901 tgggagagag tcaccacata cgaagacggg ggcgtgctga ccgctaccca ggacaccagc
     3961 ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt cacatccaac
     4021 ggccctgtga tgcagaagaa aacactcggc tgggaggcct tcaccgagac gctgtacccc
     4081 gctgacggcg gcctggaagg cagaaacgac atggccctga agctcgtggg cgggagccat
     4141 ctgatcgcaa acatcaagac cacatataga tccaagaaac ccgctaagaa cctcaagatg
     4201 cctggcgtct actatgtgga ctacagactg gaaagaatca aggaggccaa caacgagacc
     4261 tacgtcgagc agcacgaggt ggcagtggcc agatactgcg acctccctag caaactgggg
     4321 cacaagctta atacgcgtgg cagcggcgcc accaacttca gcctgctgaa gcaggccggc
     4381 gacgtggagg agaaccccgg ccccctcgag gtgcccaaga agaagaggaa agtcggcgga
     4441 catactcttt atgcccccgg tggatatgac attatgggat atctgaggca gatcaggaac
     4501 cggccaaacc cgcaggtgga actgggcccc gtggatacat cctgcgcctt gattctttgt
     4561 gacctgaaac agaaagacac cccgatagtt tacgcgagtg aagccttcct ctacatgaca
     4621 ggttacagca acgcagaggt gctgggccgg aattgccggt ttctgcaaag ccctgacggc
     4681 atggtgaagc ccaagagcac ccggaagtac gtggatagta acacaatcaa tactatgcgc
     4741 aaggcaatcg acaggaatgc cgaggtgcag gttgaagtag tcaattttaa aaagaatgga
     4801 cagcgatttg ttaatttcct gactatgata cctgttaggg acgaaacagg cgagtatcga
     4861 tactctatgg gattccagtg cgaaacagaa ggtaccaaca ggaaatggtt ccctgctgaa
     4921 cctgaggatg tgagggacta cctcctgtac ctgcaagcca gaggcctggc tgtgaagacc
     4981 atccaacagc acctgggcca gctcaacatg ctgcacagga gatctggcct gcctcgccct
     5041 tctgactcca atgctgtgtc cctggtgatg aggagaatca gaaaggagaa tgtggatgct
     5101 ggggagagag ccaagcaggc cctggccttt gaacgcactg actttgacca agtcagatcc
     5161 ctgatggaga actctgacag atgccaggac atcaggaacc tggccttcct gggcattgcc
     5221 tacaacaccc tgctgcgcat tgccgaaatt gccagaatca gagtgaagga catctcccgc
     5281 accgatggtg ggagaatgct gatccacatt ggcaggacca agaccctggt gtccacagct
     5341 ggtgtggaga aggccctgtc cctgggggtt accaagctgg tggagagatg gatctctgtg
     5401 tctggtgtgg ctgatgaccc caacaactac ctgttctgcc gggtcagaaa gaatggtgtg
     5461 gctgcccctt ctgccacctc ccaactgtcc acccgggccc tggaagggat ctttgaggcc
     5521 acccaccgcc tgatctatgg tgccaaggat gactctgggc agagatacct ggcctggtct
     5581 ggccactctg ccagagtggg tgctgccagg gacatggcca gggctggtgt gtccatccct
     5641 gaaatcatgc aggctggtgg ctggaccaat gtgaacattg tgatgaacta catcagaaac
     5701 ctggactctg agactggggc catggtgagg ctgctcgagg atggggacta ataggaattc
     5761 gaagcttgag ctcgagatct gagtccggta gcgctagttc gaaggatccg catgcatcta
     5821 gggcggccaa ttccgcccct ctcccccccc cccctctccc tccccccccc ctaacgttac
     5881 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
     5941 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
     6001 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
     6061 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
     6121 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
     6181 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
     6241 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
     6301 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
     6361 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
     6421 ataagcttgc cacaacccac aaggagacga ccttccatga ccgagtacaa gcccacggtg
     6481 cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc
     6541 gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag
     6601 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     6661 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     6721 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     6781 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     6841 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     6901 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     6961 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     7021 cgcacctggt gcatgacccg caagcccggt gcctgataat aggcggccgc tcgagaccta
     7081 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
     7141 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga
     7201 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     7261 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     7321 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     7381 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7441 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     7501 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     7561 aggccttgac attataatag atttagcagg aattgaacta ggagtggagc acacaggcaa
     7621 agttctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     7681 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     7741 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     7801 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     7861 gtgggctcta tggcttctga ggcggaaaga accagctggg ggcgcgcccc tcgaggccgc
     7921 catggtcata gctgtttgac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     7981 atttgtttat ttttctaaat acattcaaat atgtatccgc tcacgagaca ataaccctga
     8041 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     8101 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     8161 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     8221 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     8281 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     8341 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     8401 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     8461 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     8521 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     8581 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     8641 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     8701 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     8761 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     8821 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     8881 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     8941 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     9001 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     9061 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     9121 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     9181 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     9241 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     9301 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     9361 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     9421 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     9481 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     9541 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     9601 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     9661 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ct
//
