LOCUS       sequence-381062-        9963 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ERT2-CreN59-nMag-NLS with mCherry marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9963)
  AUTHORS   Allen ME, Zhou W, Thangaraj J, Kyriakakis P, Wu Y, Huang Z, Ho P, 
            Pan Y, Limsakul P, Xu X, Wang Y
  TITLE     An AND-Gated Drug and Photoactivatable Cre-loxP System for 
            Spatiotemporal Control in Cell-Based Therapeutics.
  JOURNAL   ACS Synth Biol. 2019 Oct 18;8(10):2359-2371. doi: 
            10.1021/acssynbio.9b00175. Epub 2019 Oct 8.
  PUBMED    31592660
REFERENCE   2  (bases 1 to 9963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00175"; journalName: "ACS Synth Biol"; date: 
            "2019-10-18- 18"; volume: "8"; issue: "10"; pages: "2359-2371"
COMMENT     Sequence Label: pSin_EF1a_ERT2_CreN59_nMag_P2A_mCh_IRES_PuroR
FEATURES             Location/Qualifiers
     source          1..9963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             128..761
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    808..933
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1426..1659
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1844..1888
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2175..2292
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2421..3599
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2652..3590
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3547..3567
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3625..4560
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             5221..5277
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5284..5991
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(5426..5444)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5694..5713
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    join(6090..6664,6665..6667,6668..6676)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6257..6274)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6484..6503
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6696..7295
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6696..6715)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7192..7212
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7498..7731
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7882..7899)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7888..8112
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      8155..8164
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        8208..8312
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8313..8381,8382..9173)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8531..8550)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9344..9932
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9833..9852
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggccttttgc acggaccgcc actgccaatt acctgtggtt tcatttactc taaacctgtg
       61 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
      121 tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc
      181 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
      241 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
      301 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
      361 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
      421 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
      481 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
      541 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
      601 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
      661 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
      721 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
      781 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
      841 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
      901 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
      961 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     1021 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     1081 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     1141 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     1201 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     1261 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     1321 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     1381 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     1441 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     1501 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     1561 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     1621 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     1681 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     1741 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     1801 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     1861 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     1921 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     1981 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     2041 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     2101 agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc acaaatggca
     2161 gtattcatcc acaattttaa aagaaagggg gggattgggg ggtacagtgc aggggaaaga
     2221 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     2281 attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggat cgataagctt
     2341 tgcaaagatg gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg
     2401 tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
     2461 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc
     2521 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     2581 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     2641 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg
     2701 gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc
     2761 ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc
     2821 gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc
     2881 ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg
     2941 ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg
     3001 gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt
     3061 cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg
     3121 gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa
     3181 ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg
     3241 cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac
     3301 aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg
     3361 cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg
     3421 gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc
     3481 cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt
     3541 tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag
     3601 gaattgatcc ttcgaactag tatggctgga gacatgagag ctgccaacct ttggccaagc
     3661 ccgctcatga tcaaacgctc taagaagaac agcctggcct tgtccctgac ggccgaccag
     3721 atggtcagtg ccttgttgga tgctgagccc cccatactct attccgagta tgatcctacc
     3781 agacccttca gtgaagcttc gatgatgggc ttactgacca acctggcaga cagggagctg
     3841 gttcacatga tcaactgggc gaagagggtg ccaggctttg tggatttgac cctccatgat
     3901 caggtccacc ttctagaatg tgcctggcta gagatcctga tgattggtct cgtctggcgc
     3961 tccatggagc acccagtgaa gctactgttt gctcctaact tgctcttgga caggaaccag
     4021 ggaaaatgtg tagagggcat ggtggagatc ttcgacatgc tgctggctac atcatctcgg
     4081 ttccgcatga tgaatctgca gggagaggag tttgtgtgcc tcaaatctat tattttgctt
     4141 aattctggag tgtacacatt tctgtccagc accctgaagt ctctggaaga gaaggaccat
     4201 atccaccgag tcctggacaa gatcacagac actttgatcc acctgatggc caaggcaggc
     4261 ctgaccctgc agcagcagca ccagcggctg gcccagctcc tcctcatcct ctcccacatc
     4321 aggcacatga gtaacaaagg catggagcat ctgtacagca tgaagtgcaa gaacgtggtg
     4381 cccctctatg acctgctgct ggaggcggcg gacgcccacc gcctacatgc gcccactagc
     4441 cgtggagggg catccgtgga ggagacggac caaagccact tggccactgc gggctctact
     4501 tcatcgcatt ccttgcaaaa gtattacatc acgggggagg cagagggttt ccctgccaca
     4561 gctgtcgaca atttactgac cgtacaccaa aatttgcctg cattaccggt cgatgcaacg
     4621 agtgatgagg ttcgcaagaa cctgatggac atgttcaggg atcgccaggc gttttctgag
     4681 catacctgga aaatgcttct gtccgtttgc cggtcgtggg cggcatggtg caagttgaat
     4741 ggtacccata ctctttatgc ccccggtgga tatgacatta tgggatatct ggaccagatc
     4801 ggcaaccggc caaacccgca ggtggaactg ggccccgtgg atacatcctg cgccttgatt
     4861 ctttgtgacc tgaaacagaa agacaccccg atagtttacg cgagtgaagc cttcctctac
     4921 atgacaggtt acagcaacgc agaggtgctg ggccggaatt gccggtttct gcaaagccct
     4981 gacggcatgg tgaagcccaa gagcacccgg aagtacgtgg atagtaacac aatcaatact
     5041 atgcgcaagg caatcgacag gaatgccgag gtgcaggttg aagtagtcaa ttttaaaaag
     5101 aatggacagc gatttgttaa tttcctgact atgatacctg ttagggacga aacaggcgag
     5161 tatcgatact ctatgggatt ccagtgcgaa acagaaggag ggtctggagg gggatccggc
     5221 gccaccaact tcagcctgct gaagcaggcc ggcgacgtgg aggagaaccc cggccccacg
     5281 cgtgtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
     5341 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
     5401 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
     5461 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
     5521 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
     5581 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
     5641 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
     5701 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
     5761 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
     5821 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
     5881 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
     5941 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta ataggaattc
     6001 gaagcttgag ctcgagatct gagtccggta gcgctagttc gaaggatccg catgcatcta
     6061 gggcggccaa ttccgcccct ctcccccccc ccctctccct cccccccccc taacgttact
     6121 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     6181 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     6241 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     6301 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     6361 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     6421 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     6481 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     6541 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
     6601 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
     6661 taagcttgcc acaacccaca aggagacgac cttccatgac cgagtacaag cccacggtgc
     6721 gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg
     6781 actaccccgc cacgcgccac accgtcgacc cggaccgcca catcgagcgg gtcaccgagc
     6841 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     6901 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     6961 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     7021 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     7081 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     7141 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     7201 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     7261 gcacctggtg catgacccgc aagcccggtg cctgataata ggcggccgct cgagacctag
     7321 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
     7381 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
     7441 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     7501 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     7561 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7621 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     7681 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     7741 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     7801 ggccttgaca ttataataga tttagcagga attgaactag gagtggagca cacaggcaaa
     7861 gttctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     7921 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     7981 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     8041 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     8101 tgggctctat ggcttctgag gcggaaagaa ccagctgggg gcgcgcccct cgaggccgcc
     8161 atggtcatag ctgtttgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     8221 tttgtttatt tttctaaata cattcaaata tgtatccgct cacgagacaa taaccctgat
     8281 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     8341 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     8401 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     8461 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     8521 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     8581 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     8641 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     8701 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     8761 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     8821 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     8881 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     8941 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     9001 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     9061 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     9121 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     9181 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     9241 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     9301 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     9361 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     9421 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     9481 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     9541 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     9601 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     9661 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     9721 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     9781 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     9841 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     9901 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     9961 tct
//
