LOCUS       sequence-381070-       10633 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing nuclease-dead Cas9 from Neisseria 
            meningitis (dNme2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10633)
  AUTHORS   Edraki A, Mir A, Ibraheim R, Gainetdinov I, Yoon Y, Song CQ, Cao Y, 
            Gallant J, Xue W, Rivera-Perez JA, Sontheimer EJ
  TITLE     A Compact, High-Accuracy Cas9 with a Dinucleotide PAM for In Vivo 
            Genome Editing.
  JOURNAL   Mol Cell. 2018 Dec 18. pii: S1097-2765(18)31033-5. doi: 
            10.1016/j.molcel.2018.12.003.
  PUBMED    30581144
REFERENCE   2  (bases 1 to 10633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2018 Dec 18. pii: S1097-2765(18)31033-5. doi: 
            10.1016/j.molcel.2018.12.003."
COMMENT     Sequence Label: pSFFV-dNme2Cas9
FEATURES             Location/Qualifiers
     source          1..10633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        82..308
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             309..489
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    536..661
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1153..1386
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1571..1615
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1882..1999
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2741..3147
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      3202..3211
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3214..3234
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3241..3288
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6541..6588
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6589..6678
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             6685..6711
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     promoter        6741..6952
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             6968..7567
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6968..6987)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7464..7484
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7581..8169
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7634..7654)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8052..8063)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(8245..8278)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(8483..8500)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8489..8713
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8786..8803)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8957..9545)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9037..9056)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9716..10507,10508..10576))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10339..10358
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10577..10633,1..48))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
       61 aaaagtgcca cctgacgtca atgtagtctt atgcaatact cttgtagtct tgcaacatgg
      121 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg
      181 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
      241 ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat
      301 acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
      361 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
      421 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
      481 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
      541 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
      601 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
      661 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
      721 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
      781 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
      841 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
      901 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
      961 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccctgatct tcagacctgg
     1021 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     1081 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     1141 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     1201 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     1261 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     1321 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     1381 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     1441 tgctagttgg agtaatgaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     1501 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     1561 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     1621 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     1681 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     1741 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     1801 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     1861 atctcgacgg tatcggttaa cttttaaaag aaaagggggg attggggggt acagtgcagg
     1921 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1981 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggatcga
     2041 taagcttgat atcgaattcg ggaggtggtc cctgcagtta cgccaatgat aacccccgcc
     2101 agaaaaatct tagtagcctt ccctttttgt tttccgtgcc ccaactcggc ggattgactc
     2161 ggccccttcc ggaaacaccc gaatcaactt ctagtcaaat tattgttcac gccgcaatga
     2221 cccacccctg gcccgcgtct gtggaactga cccctggtgt acaggagagt tcgctgctga
     2281 aagtggtccc aaaggggtac tagtttttaa gctcccaact ccccctcccc cagcgtctgg
     2341 aggattccac accctcgcac cgcaggggcg aggaagtggg cggagtccgg ttttggcgcc
     2401 agccgctgag gctgccaagc agaaaagcca ccgctgagga gactccggtc actgtcctcg
     2461 ccccgcctcc cccttccctc cccttgggga ccaccgggcg ccacgccgcg aacggtaagt
     2521 gccgcggtcg tcggcgcctc cgccctcccc ctagggcccc aattcccagc gggcgcggcg
     2581 cgcggccccg cgcgcagctc ccggctccct cccccttcgg atgtggcttg agctgtaggc
     2641 gcggagtcct gcagccccga taaaataaaa gattttattt agtctccaga aaaagggggg
     2701 aatgaaagac cccacctgta ggtttggcaa gctagctgca gtaacgccat tttgcaaggc
     2761 atggaaaaat accaaaccaa gaatagagaa gttcagatca agggcgggta catgaaaata
     2821 gctaacgttg ggccaaacag gatatctgcg gtgagcagtt tcggccccgg cccggggcca
     2881 agaacagatg gtcaccgcag tttcggcccc ggcccgaggc caagaacaga tggtccccag
     2941 atatggccca accctcagca gtttcttaag acccatcaga tgtttccagg ctcccccaag
     3001 gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc agcctgcttc tcgcttctgt
     3061 tcgcgcgctt ctgcttcccg agctctataa aagagctcac aacccctcac tcggcgcgcc
     3121 agtcctccga cagactgagt cgcccggtaa gcagacagtg gttcaaagtt tttttcttcc
     3181 atttcaggtg tcgtgaacac cgccaccatg gtgcctaaga agaagagaaa ggtggaagat
     3241 aaacgcccag cagctacaaa gaaggcaggt caagccaaga aaaagaaaat ggccgccttc
     3301 aagcctaacc caatcaatta catcctggga ctggccatcg gaatcgcatc cgtgggatgg
     3361 gctatggtgg agatcgacga ggaggagaat cctatccggc tgatcgatct gggcgtgaga
     3421 gtgtttgaga gggccgaggt gccaaagacc ggcgattctc tggctatggc ccggagactg
     3481 gcacggagcg tgaggcgcct gacacggaga agggcacaca ggctgctgag ggcacgccgg
     3541 ctgctgaaga gagagggcgt gctgcaggca gcagacttcg atgagaatgg cctgatcaag
     3601 agcctgccaa acaccccctg gcagctgaga gcagccgccc tggacaggaa gctgacacca
     3661 ctggagtggt ctgccgtgct gctgcacctg atcaagcacc gcggctacct gagccagcgg
     3721 aagaacgagg gagagacagc agacaaggag ctgggcgccc tgctgaaggg agtggccaac
     3781 aatgcccacg ccctgcagac cggcgatttc aggacacctg ccgagctggc cctgaataag
     3841 tttgagaagg agtccggcca catcagaaac cagaggggcg actatagcca caccttctcc
     3901 cgcaaggatc tgcaggccga gctgatcctg ctgttcgaga agcagaagga gtttggcaat
     3961 ccacacgtga gcggaggcct gaaggaggga atcgagaccc tgctgatgac acagaggcct
     4021 gccctgtccg gcgacgcagt gcagaagatg ctgggacact gcaccttcga gcctgcagag
     4081 ccaaaggccg ccaagaacac ctacacagcc gagcggttta tctggctgac aaagctgaac
     4141 aatctgagaa tcctggagca gggatccgag aggccactga ccgacacaga gagggccacc
     4201 ctgatggatg agccttaccg gaagtctaag ctgacatatg cccaggccag aaagctgctg
     4261 ggcctggagg acaccgcctt ctttaagggc ctgagatacg gcaaggataa tgccgaggcc
     4321 tccacactga tggagatgaa ggcctatcac gccatctctc gcgccctgga gaaggagggc
     4381 ctgaaggaca agaagtcccc cctgaacctg agctccgagc tgcaggatga gatcggcacc
     4441 gccttctctc tgtttaagac cgacgaggat atcacaggcc gcctgaagga cagggtgcag
     4501 cctgagatcc tggaggccct gctgaagcac atctctttcg ataagtttgt gcagatcagc
     4561 ctgaaggccc tgagaaggat cgtgccactg atggagcagg gcaagcggta cgacgaggcc
     4621 tgcgccgaga tctacggcga tcactatggc aagaagaaca cagaggagaa gatctatctg
     4681 ccccctatcc ctgccgacga gatcagaaat cctgtggtgc tgagggccct gtcccaggca
     4741 agaaaagtga tcaacggagt ggtgcgccgg tacggatctc cagcccggat ccacatcgag
     4801 accgccagag aagtgggcaa gagcttcaag gaccggaagg agatcgagaa gagacaggag
     4861 gagaatcgca aggatcggga gaaggccgcc gccaagttta gggagtactt ccctaacttt
     4921 gtgggcgagc caaagtctaa ggacatcctg aagctgcgcc tgtacgagca gcagcacggc
     4981 aagtgtctgt atagcggcaa ggagatcaat ctggtgcggc tgaacgagaa gggctatgtg
     5041 gagatcgatg ccgccctgcc tttctccaga acctgggacg attcttttaa caataaggtg
     5101 ctggtgctgg gcagcgagaa ccagaataag ggcaatcaga caccatacga gtatttcaat
     5161 ggcaaggaca actccaggga gtggcaggag ttcaaggccc gcgtggagac ctctagattt
     5221 cccaggagca agaagcagcg gatcctgctg cagaagttcg acgaggatgg ctttaaggag
     5281 tgcaacctga atgacaccag atacgtgaac cggttcctgt gccagtttgt ggccgatcac
     5341 atcctgctga ccggcaaggg caagagaagg gtgttcgcct ctaatggcca gatcacaaac
     5401 ctgctgaggg gattttgggg actgaggaag gtgcgggcag agaatgacag acaccacgca
     5461 ctggatgcag tggtggtggc atgcagcacc gtggcaatgc agcagaagat cacaagattc
     5521 gtgaggtata aggagatgaa cgcctttgac ggcaagacca tcgataagga gacaggcaag
     5581 gtgctgcacc agaagaccca cttcccccag ccttgggagt tctttgccca ggaagtgatg
     5641 atccgggtgt tcggcaagcc agacggcaag cctgagtttg aggaggccga taccccagag
     5701 aagctgagga cactgctggc agagaagctg tctagcaggc cagaggcagt gcacgagtac
     5761 gtgaccccac tgttcgtgtc cagggcaccc aatcggaaga tgtctggcgc ccacaaggac
     5821 acactgagaa gcgccaagag gtttgtgaag cacaacgaga agatctccgt gaagagagtg
     5881 tggctgaccg agatcaagct ggccgatctg gagaacatgg tgaattacaa gaacggcagg
     5941 gagatcgagc tgtatgaggc cctgaaggca aggctggagg cctacggagg aaatgccaag
     6001 caggccttcg acccaaagga taaccccttt tataagaagg gaggacagct ggtgaaggcc
     6061 gtgcgggtgg agaagaccca ggagagcggc gtgctgctga ataagaagaa cgcctacaca
     6121 atcgccgaca atggcgatat ggtgagagtg gacgtgttct gtaaggtgga taagaagggc
     6181 aagaatcagt actttatcgt gcctatctat gcctggcagg tggccgagaa catcctgcca
     6241 gacatcgatt gcaagggcta cagaatcgac gatagctata cattctgttt ttccctgcac
     6301 aagtatgacc tgatcgcctt ccagaaggat gagaagtcca aggtggagtt tgcctactat
     6361 atcaattgcg actcctctaa cggcaggttc tacctggcct ggcacgataa gggcagcaag
     6421 gagcagcagt ttcgcatctc cacccagaat ctggtgctga tccagaagta tcaggtgaac
     6481 gagctgggca aggagatcag gccatgtcgg ctgaagaagc gcccacccgt gcgggaggat
     6541 aagcggcccg cagcaaccaa gaaggcagga caggccaaga agaagaagta cccctatgac
     6601 gtgcctgact acgccgggta tccctacgac gtgcctgatt acgccgggtc ctatccctac
     6661 gacgtgccag attacgctgc agctccagca gcgaagaaaa agaagctgga ttaaatgcag
     6721 aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     6781 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
     6841 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     6901 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggatcctcg
     6961 agccaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg
     7021 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     7081 cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     7141 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     7201 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     7261 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     7321 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     7381 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     7441 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     7501 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     7561 tgcctgaatc tagtgtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     7621 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     7681 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     7741 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     7801 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     7861 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     7921 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     7981 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     8041 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     8101 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     8161 cctccccgcc tggaattcgg taccttttta aaagaaaagg ggggactgga agggctaatt
     8221 cactcccaac gaagacaaga tatcataact tcgtatagca tacattatac gaagttataa
     8281 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccatatgttt atttgtgaaa
     8341 tttgtgatgc tattgcttta tttgtaacca ttgctttttg cttgtactgg gtctctctgg
     8401 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     8461 caataaagct tgcctcgacc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     8521 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     8581 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     8641 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     8701 gtgggctcta tggcctgcag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     8761 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     8821 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     8881 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     8941 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     9001 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     9061 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     9121 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     9181 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     9241 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     9301 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     9361 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     9421 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     9481 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     9541 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     9601 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     9661 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     9721 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     9781 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     9841 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     9901 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     9961 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    10021 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    10081 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    10141 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    10201 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    10261 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    10321 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    10381 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    10441 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    10501 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    10561 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    10621 gtctcatgag cgg
//
